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Method for determining form of selenium in selenium-enriched edible fungi

A technology of selenium-enriched edible fungi and a determination method is applied in the field of determination of selenium form after pretreatment of selenium-enriched edible fungi, which can solve the problems of unclear organic selenium form, complicated operation steps, poor repeatability, etc. The effect of improving extraction efficiency and low cost

Inactive Publication Date: 2012-06-27
SUZHOU SETEK
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Problems solved by technology

[0004] This method has cumbersome operation steps and poor repeatability, and can only obtain the total amount of organic selenium. The specific form of organic selenium is not clear, and organic solvents such as cyclohexane and toluene used to extract organic selenium have certain toxicity.
[0005] Selenium speciation research belongs to cutting-edge research, Selenium species in leaves of chicory, dandelion, lamb's lettuce and parsley (Darja Mazej, Vekoslava Stibilj, et al.Food Chemistry, 2008, 107: 75-83.) discloses a method of using protease hydrolysis alone The sample is then carried out the method of selenium speciation test, and the ratio of this method protease consumption and sample is 1: 2~1: 5, and cost is very high

Method used

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  • Method for determining form of selenium in selenium-enriched edible fungi
  • Method for determining form of selenium in selenium-enriched edible fungi
  • Method for determining form of selenium in selenium-enriched edible fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Selenium form determination in embodiment 1 selenium-enriched Pleurotus eryngii

[0057] The steps of the pretreatment method for the determination of selenium speciation in selenium-enriched Pleurotus eryngii were optimized. The optimization process and results are as follows:

[0058] Weigh 0.2g selenium-enriched Pleurotus eryngii into a 15mL plastic centrifuge tube, add 10mL deionized water as the extraction solution, ultrasonically extract for 20min, add 0.5mg proteinase K, culture at 50°C for 8h at constant temperature, then add 0.5mg protease XIV, 37 Centrifuge at 10,000 rpm for 30 minutes at 4°C, and then pass through a 0.22 μm filter membrane to prepare the test solution for the machine.

[0059] Set the liquid chromatography conditions as mobile phase (40mmol / L(NH4)2HPO4, pH 6.0); flow rate 1mL / min; injection volume 100μL; peristaltic pump speed 65rpm; lamp current / auxiliary cathode: 100mA, 45mA; photomultiplier tube Negative high voltage: 300V; carrier gas fl...

Embodiment 2

[0076] Example 2 Determination of Selenium Species in Selenium-enriched Ganoderma lucidum

[0077] Weigh 0.2g selenium-enriched Ganoderma lucidum into a 15mL plastic centrifuge tube, add 5mL extraction buffer Tris-HCl, ultrasonically extract for 30min, add 1mg proteinase K, 50°C, shake at constant temperature for 12h, then add 1mg protease XIV, 37°C, constant temperature After 12 hours of shaking culture, at 4°C, at 10,000 rpm for 30 minutes, the supernatant was passed through a 0.22 μm filter membrane, and the content of organic selenium was measured on a machine. Other conditions are the same as in Example 1. The results of screening for optimal extraction conditions, determination of samples, and standard addition recovery are shown in the following table.

[0078] Table 5. Optimization condition screening and effect

[0079]

[0080] It can be seen from Table 5 that deionized water was selected as the extractant in the pretreatment method for selenium speciation determ...

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Abstract

The invention discloses a method for determining the form of selenium in selenium-enriched edible fungi. The method is characterized by comprising the following steps of: (1) pretreating selenium-enriched edible fungi: using deionized water as an extracting solution to extract a crushed selenium-enriched edible fungus sample, then adding effective amounts of protease K and protease XIV for hydrolysis so as to extract a sample solution to be detected; (2) conducting hydride generation: combining atomic fluorescence spectrometry with high performance liquid chromatography to determine a standard selenium form solution and the sample solution to be detected respectively. The method of the invention has the advantages of simplicity and easy operation, good repeatability, and employment of non-toxic and pollution-free reagents.

Description

technical field [0001] The invention relates to a method for measuring the form of selenium in selenium-enriched edible fungi, in particular to a method for measuring the form of selenium in selenium-enriched edible fungi after pretreatment. Background technique [0002] Selenium is one of the essential trace elements for the human body and a component of glutathione peroxidase (GSH-Px), which has the functions of anti-oxidation, anti-cancer, anti-aging, improving human immunity, and protecting cardiovascular and cerebrovascular ( Rotruck J T, Pope A L, Ganther H E, et al Selenium: biochemical role as a component of glutathione peroxidase. Science, 1973, 179, 588-590.). There are two-thirds of selenium-deficient areas in my country, and one-third of them are severely selenium-deficient areas. Selenium deficiency in humans and animals can cause Keshan disease, Kaschin-Beck disease, white muscle disease of animals, etc. In addition, some cancers, endemic thyroid disorders and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N30/06
Inventor 朱元元
Owner SUZHOU SETEK
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