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Method for preparing active peptide of laver

A technology of active peptides and seaweed, applied in the direction of fermentation, can solve the problems of complicated operation and difficult to achieve, and achieve the effects of improving the extraction rate, prolonging the extraction time, and good blood pressure lowering activity.

Inactive Publication Date: 2011-08-17
HUAIHAI INST OF TECH
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AI Technical Summary

Problems solved by technology

The core technology of this invention is the enzyme-membrane coupling technology, and the product yield is improved, but there are also complex operations and the defects that are difficult to realize in actual production, which is different from the papain used in the present invention.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1. A preparation method of laver active peptide, the steps are as follows:

[0017] (1) Take Porphyra zebra as raw material, crush it after drying, sieve, take 150-200 mesh part, add water to the raw material according to the weight volume ratio of raw material and water 1:100 g / mL and mix evenly; then mix the raw material liquid Put it into an ultrasonic cell pulverizer for crushing treatment, the ultrasonic power is 650W, the cavitation ratio is 30%, the temperature of the material liquid is kept at 50°C, and the crushing treatment time is 15 minutes; the material after ultrasonic treatment is centrifuged at 4000r / min for 10 minutes in a centrifuge to obtain supernatant;

[0018] (2) Take the supernatant and adjust the pH to 7.0 with 1mol / L NaOH, and add the enzyme activity to 3×10 5 U / g of papain, mix well and place it in a water bath at 55°C for hydrolysis, stir and oscillate once every 20 minutes, monitor the change of pH during the reaction process, add ...

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PUM

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Abstract

The invention discloses a method for preparing active peptide of laver. The method is characterized by comprising the following steps of: drying and crushing porphyra yezoensis serving as a raw material, taking the part with particle size of between 150 and 200 meshes, adding water into the raw material according to a weight volume ratio of the raw material to water as 1 to 100g / mL and uniformly mixing; filling the material liquid into an ultrasonic cell disruptor for disruption and centrifuging to obtain supernatant; adjusting the pH value of the supernatant to be 7.0, adding papain for enzymolysis, inactivating, cooling and centrifuging to obtain enzymatic hydrolyzate; and performing ultrafiltration on the enzymatic hydrolyzate, concentrating filtrate and performing vacuum freeze dryingto obtain a freeze-dried product of the active peptide of the laver. The method has the advantages of more reasonable preparation process, simple process, high operability and high extraction rate; the prepared active peptide of the laver contains fewer impurities; and the biological activity is also kept to the greatest extent.

Description

technical field [0001] The invention relates to a preparation method of an active peptide, in particular to a preparation method of a laver active peptide. Background technique [0002] The Chinese published patent document with the publication number CN1687444 and the application number CN200510038532.5 discloses a "method for preparing laver hypotensive peptide using enzyme-membrane coupling technology and its application", which is the preparation of laver antihypertensive peptide by enzyme-membrane coupling technology. In the method of the blood pressure peptide, the proteases selected are trypsin, compound protease, pepsin, alkaline protease, neutral protease and the compound enzymes composed of them. The core technology of this invention is the enzyme-membrane coupling technology, and the product yield is improved, but it also has the disadvantages of complicated operation and difficult realization in actual production, which is different from the papain used in the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 姚兴存舒留泉盘赛昆
Owner HUAIHAI INST OF TECH
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