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Method for preparing composite amino acid liquid using waste protein

A technology for compound amino acid and waste protein, applied in the field of preparing compound amino acid liquid, can solve the problems of damage, increase production cost, long hydrolysis time, etc., and achieve the effects of high hydrolysis efficiency, low cost and simple operation.

Inactive Publication Date: 2006-02-15
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amino acids mentioned above are all prepared by a single acid or enzyme; when the acid is used for hydrolysis, the carcinogen-chloropropanol will be produced during the hydrolysis process, and some amino acid components, such as tryptophan, will be destroyed at the same time; However, when only microbial protease is used for hydrolysis, the production cost will be increased, and the hydrolysis time will be longer

Method used

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  • Method for preparing composite amino acid liquid using waste protein

Examples

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Effect test

Embodiment 1

[0013] a. Raw material pretreatment: First, after removing impurities, crushing soybean meal, rapeseed cake and cottonseed cake, sieving (20-100 mesh), soaking with 200% (weight percent) dilute sulfuric acid for 12 hours, and filtering off the supernatant liquid, steamed for 1 hour. The waste yeast is washed and centrifuged to collect the yeast sludge and refrigerate for future use.

[0014] b. Mixing: Mix the above-mentioned processed vegetable protein materials uniformly in a ratio of 1:1:0.3 (weight ratio), and control the moisture content of the mixture within 50%. Then the mixture is mixed with the treated yeast mud at a ratio of 1:0.5 (weight ratio).

[0015] c. Enzyme hydrolysis: add 5-8 times of water to it, add neutral protease according to the addition amount of 2000u / g raw materials, and perform constant temperature enzymolysis under the condition of 45-52°C for 4-6 hours.

[0016] d. Acid hydrolysis: Add food-grade pure hydrochloric acid to the above mixed soluti...

Embodiment 2

[0020] Embodiment 2: repeat embodiment 1, have following difference: in the raw material pretreatment step, soak 20 hours with the dilute sulfuric acid of 200% (percentage by weight), boil 30 minutes. The results showed that the yield of amino acid was 11.2%, and the taste was slightly worse than that of Example 1.

Embodiment 3

[0021] Embodiment 3: repeat embodiment 1, have following difference: in the raw material pretreatment step, soak 20 hours with the dilute sulfuric acid of 200% (percentage by weight), cook for 2 hours. The results showed that the yield of amino acid was 11.7%, and the taste was slightly worse than that of Example 1.

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Abstract

The invention discloses a method to prepare composite amino acid liquid by abandoned protein, which comprises steps: a. pretreating the material, smashing and removing impurity to soya bean waste, rape seed cake and cotton cake; sieving, dipping with dilute sulphuric acid or muriatic acid; filtering supernatant and cooking; b. mixing material; c. hydrolyzing with neutral proteased at thermostatic condition; d. hydrolyzing with edible pure muriatic acid on condition of thermostatic and pressure; e. neutralizing with edible caustic soda, filtering, and adjusting with caustic soda solution pH value to 4.5~5.0; f. matching and mixing. This method is simple and high efficient.

Description

Technical field: [0001] The invention relates to a method for preparing compound amino acid liquid by using waste protein mixture as raw material and utilizing an acid-enzyme hydrolysis method. Background technique: [0002] As a protein supplement, compound amino acid liquid contains various amino acids and polypeptides needed by the human body, can be absorbed by the human body quickly and in multiple ways, and has special physiological functions such as detoxification and blood pressure reduction, so it has received great attention from people. It is widely used in food, feed, pharmaceutical fermentation and cosmetics industries. [0003] At present, the commonly used methods for hydrolyzing vegetable protein include acid method, alkali method and enzymatic method. Among them, acid method and enzymatic method have been studied more. Enzymatic hydrolysis has mild conditions, less side reactions, no destruction of amino acids, and easy control of hydrolysis. For example, C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04
Inventor 周剑平魏甲乾孙智敏王治业杨晖
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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