Method for preparing bacterial cellulose by using wheat straw

A technology of bacterial cellulose and wheat straw, which is applied in the field of bacterial cellulose preparation, can solve the problems of straw dissolution that have not been reported in detail, and achieve the effect of improving efficiency

Active Publication Date: 2011-03-16
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(AMIM)Cl has not been reported in detail for its use in the dissolution of straw

Method used

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  • Method for preparing bacterial cellulose by using wheat straw
  • Method for preparing bacterial cellulose by using wheat straw
  • Method for preparing bacterial cellulose by using wheat straw

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Pretreatment of wheat straw

[0040] At 100°C and 500 rpm magnetic stirring speed, 150 mg of 40-60 mesh wheat straw was placed in 1.5 g, 3 g, and 5 g ionic liquid (AMIM) Cl, respectively, and pretreated for 2 h.

[0041] (2) Regeneration of wheat straw

[0042] Add 5 times the volume of deionized water to the ionic liquid mixture after the above treatment, stir for 30 min, centrifuge the mixture at 4000 rpm for 10 min, and wash the precipitate repeatedly until the supernatant is colorless and transparent.

[0043] (3) Enzymatic hydrolysis of regenerated wheat straw

[0044] Completely transfer 150mg of precipitate to a 50ml Erlenmeyer flask, add 30ml, 50mmol pH 4.8 acetate buffer, then add 90mg of cellulase (11000U / g), put it in a shaking water bath, 50℃, 100rpm for enzymatic hydrolysis For the reaction, suck the reaction solution at regular intervals (respectively 2, 6, 12, 24h), centrifuge at 4000-10000rpm for 5-20min to separate the undegraded wheat straw and the enzymati...

Embodiment 2

[0049] (1) Pretreatment of wheat straw

[0050] 100 ℃, 500 rpm magnetic stirring speed, put 150mg of wheat straw of different meshes (40-60 mesh, 60-80 mesh, 80 mesh and above, mixed mesh) in 50ml containing 5g of ionic liquid (AMIM) Cl Pretreatment for 2h in a triangular Erlenmeyer flask.

[0051] (2) Regeneration of wheat straw

[0052] Add 5 times the volume of deionized water to the ionic liquid mixture after the above treatment, stir for 30 min, pour the mixture into a Buchner funnel, and filter the filter residue repeatedly until the filtrate is colorless and transparent. Collect the residue And the filtrate for solution.

[0053] (3) Distillation recovery of ionic liquid

[0054] Place the collected filtrate in a distillation flask, and distill under reduced pressure at a temperature of 130°C and a vacuum of -0.09~-0.1Mpa to remove the water blended with the ionic liquid. Distill until no water can be evaporated, and the remaining in the distillation flask Is the recovered ion...

Embodiment 3

[0059] (1) Pretreatment of wheat straw

[0060] Put 150mg of 40-60 mesh wheat straw in 5g of ionic liquid (AMIM) Cl at four temperatures of 90℃, 100℃, 110℃, and 120℃, and treat them at 90℃ under magnetic stirring at 500rpm. 4, 6, 8 , 10, 12h; 100℃ treatment for 2, 2.5, 3, 3.5, 4h; 110℃ treatment for 1, 1.5, 1.75, 2, 2.5h; 120℃ treatment for 15, 30, 45, 60, 75min.

[0061] (2) Regeneration of wheat straw

[0062] Add 5 times the volume of deionized water to the ionic liquid mixture to the conical flask after the above treatment, stir for 30 min, centrifuge the mixture at 4000 rpm for 10 min, and rinse the precipitate repeatedly until the supernatant is colorless and transparent.

[0063] (3) Enzymatic hydrolysis of regenerated wheat straw

[0064] Completely transfer 150mg of precipitate to a 50ml Erlenmeyer flask, add 30ml, 50mmol pH 4.8 acetate buffer, then add 90mg of cellulase (11U / mg), put in a shaking water bath, 50℃, 100rpm for enzymatic hydrolysis After 6h, after the reaction, ...

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Abstract

The invention relates to a method for preparing bacterial cellulose by using wheat straw, which comprises: pretreating the wheat straw in ionic liquid at 90 to 120 DEG C for 15 minutes to 21 hours; adding deionized water; stirring for regeneration; adding cellulose for enzymolysis, and obtaining enzymolysis solution; detoxifying the enzymolysis solution to obtain the culture medium for culturing the bacterial cellulose; adding yeast extract and tryptone to prepare the culture medium; adding seed liquid of bacterial cellulose producing bacteria; and culturing the bacteria at 20 to 30 DEG C for 8 to 28 days and obtaining the bacterial cellulose. In the invention, usually waste or burnt crop straw byproducts are used as raw materials, a cheap carbon source is developed, environmental pollution is reduced, and the bacterial cellulose can be used for producing products with high added values and can create good economic benefit for the related industry.

Description

Technical field [0001] The invention belongs to the field of preparation of bacterial cellulose, and particularly relates to a method for preparing bacterial cellulose by using wheat straw. Background technique [0002] Bacterial cellulose (BC) is a kind of cellulose produced by microorganisms. Compared with plant fibers, bacterial cellulose has the characteristics of high crystallinity, high water holding capacity, high Young's modulus, high purity, and excellent biocompatibility. Therefore, bacterial cellulose is used in medical dressings and other biomedical materials, food, and papermaking. , Textile and other fields have broad applications. However, the high production cost of bacterial cellulose limits its industrial scale, so reducing the cost of bacterial cellulose is an urgent problem to be solved in current industrial production. One of the main factors for the high cost of bacterial cellulose is the high cost of carbon sources in the culture medium. Therefore, this e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12P19/14C12R1/02C12R1/01C12R1/41C12R1/38C12R1/025C12R1/05C12R1/065
Inventor 洪枫韩士芬杨雪霞杨光曹张军
Owner DONGHUA UNIV
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