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Method for preparing rapeseed dreg protein antioxidative peptide solution by gastrointestinal simulated digestion

A technology for simulating digestion and anti-oxidative peptides, applied in the field of food processing technology and protein utilization, can solve the problems of unclear correlation between the activity of the enzymatic hydrolyzate product and the molecular weight of the polypeptide, and the low efficiency of single-enzyme enzymatic hydrolysis, so as to make full use of raw materials and improve The effect of high hydrolysis efficiency and enzymatic hydrolysis efficiency

Active Publication Date: 2015-01-07
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the problems in the prior art such as the low enzymatic hydrolysis efficiency of single enzyme, the continuous adjustment of pH in the enzymatic hydrolysis reaction and the use of a large amount of NaOH for adjustment, and the lack of clear correlation between the activity of the enzymatic hydrolyzed product and the molecular weight of the polypeptide. Simulating the human gastrointestinal digestive system to construct a very safe preparation method of rapeseed meal polypeptide

Method used

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  • Method for preparing rapeseed dreg protein antioxidative peptide solution by gastrointestinal simulated digestion
  • Method for preparing rapeseed dreg protein antioxidative peptide solution by gastrointestinal simulated digestion
  • Method for preparing rapeseed dreg protein antioxidative peptide solution by gastrointestinal simulated digestion

Examples

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Effect test

Embodiment 1

[0051] Add 25 mL of simulated gastric digestion solution into a 100 mL Erlenmeyer flask, and place in a constant temperature water bath at 37°C for 5 minutes. Add rapeseed meal accounting for 4% of the mass ratio of gastric digestive juice, quickly vortex and oscillate, quickly place in a 37°C water bath, and stir for 4 hours for enzymatic hydrolysis. Then 35 mL of simulated intestinal digestion solution was added, the pH was adjusted to 6.4, and the enzyme was hydrolyzed for 1 h at a constant temperature of 37°C. Boiling water bath for 10 min to terminate the reaction; centrifuge at 4500r / min for 10min, take the supernatant to adjust the pH to 4.2, centrifuge at 4500r / min for 30min to remove macromolecular protein, and obtain the antioxidant rapeseed meal peptide solution. The scavenging rate of DPPH was 72.99±0.32%, and the scavenging rate of OH (hydroxyl radical) was 93.98±0.19%. Peptides with a molecular weight distribution of 211-5000Da in the peptide solution accounted ...

Embodiment 2

[0055] Add 25 mL of simulated gastric digestion solution into a 100 mL Erlenmeyer flask, and place in a constant temperature water bath at 37°C for 5 minutes. Add 2% rapeseed meal in the mass ratio of gastric digestive juice, quickly vortex and oscillate, quickly place in a 37°C water bath, and stir for 4 hours for enzymatic hydrolysis. Then 35 mL of simulated intestinal digestion solution was added, the pH was adjusted to 6.4, and the enzyme was hydrolyzed for 1 h at a constant temperature of 37°C. Boiling water bath for 10 min to terminate the reaction; centrifuge at 4500r / min for 10min, take the supernatant to adjust the pH to 4.2, centrifuge at 4500r / min for 30min to remove macromolecular protein, and obtain the antioxidant rapeseed meal peptide solution. The scavenging rate of DPPH was 71.65±0.78%, and the scavenging rate of OH (hydroxyl radical) was 93.21±0.65%.

Embodiment 3

[0057] Add 25 mL of simulated gastric digestion solution into a 100 mL Erlenmeyer flask, and place in a constant temperature water bath at 37°C for 5 minutes. Add 1% rapeseed meal in the mass ratio of gastric digestive juice, vortex and shake rapidly, place in a 37°C water bath, and stir for 4 hours for enzymatic hydrolysis. Then 35 mL of simulated intestinal digestion solution was added, the pH was adjusted to 6.4, and the enzyme was hydrolyzed for 1 h at a constant temperature of 37°C. Boiling water bath for 10 min to terminate the reaction; centrifuge at 4500r / min for 10min, take the supernatant to adjust the pH to 4.2, centrifuge at 4500r / min for 30min to remove macromolecular protein, and obtain the antioxidant rapeseed meal peptide liquid. The scavenging rate of DPPH was 71.12±0.28%, and the scavenging rate of OH (hydroxyl radical) was 90.23±0.73%.

[0058] Table 1 Composition and content of free amino acids in rapeseed meal raw materials and rapeseed meal antioxidant p...

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Abstract

The invention relates to a method for preparing a rapeseed dreg protein antioxidative peptide solution by gastrointestinal simulated digestion, belonging to the technical fields of food processing technique and protein utilization. The preparation technique comprises the following steps: by using the rapeseed oil manufacturing byproduct high-denatured-protein rapeseed dreg as the raw material, carrying out pulverization, screening and other conventional pretreatment, establishing a double-enzyme simulated gastrointestinal digestion system from pepsin and trypsinase by using a gastrointestinal digestion simulation technique, treating in a boiling water bath, regulating the pH value, and centrifugating twice to prepare the rapeseed dreg protein antioxidative peptide solution. By adopting the double-enzyme enzymolysis reaction system, the preparation method greatly enhances the rapeseed dreg protein hydrolysis efficiency, and fully utilizes the raw material; in the gastrointestinal digestion simulation process, NaOH is continuously added under the condition of no conventional enzymolysis to keep the reaction at the optimal pH value, so that the reaction process is green; and meanwhile, by simulating the perfect human body gastrointestinal digestion means, the method is better than microbial fermentation in the aspect of potential food safety.

Description

technical field [0001] The invention belongs to the technical fields of food processing technology and protein utilization, and in particular relates to a method for preparing rapeseed meal protein anti-oxidation peptide liquid by using gastrointestinal simulated digestion. Background technique [0002] Rapeseed meal is a by-product of rapeseed oil extraction. The crude protein content in rapeseed meal is generally above 32%, and the crude fiber content is below 12%. Its amino acid composition is rich in nutrition and balanced. Compared with soybean meal, it has an obvious price Advantages: Due to the highly denatured protein caused by high-temperature pressing and oil production, it is currently mainly developed as livestock and poultry protein feed, and it is a kind of plant protein resource with great development potential. [0003] Polypeptide is a compound made of amino acids connected by peptide bonds, usually with a molecular weight between 200 Da and 10,000 Da, and i...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 周存山马海乐余筱洁杨华杨欢秦晓佩胡佳丽
Owner JIANGSU UNIV
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