Culture method for rapidly increasing antithrombin activity of hirudo nipponia
A Japanese leech and antithrombin technology is applied in the breeding field to rapidly increase the antithrombin activity of Japanese leech, can solve the problems of low efficiency of antithrombin activity and low survival rate, and achieves convenient breeding, The effect of low breeding cost and high antithrombin activity
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Embodiment 1
[0021] A culture method for rapidly increasing the antithrombin activity of Japanese medicine leech is carried out according to the following steps:
[0022] S1: The setting of the breeding water tank, the length of the breeding water tank is 100cm, the width of the breeding water tank is 40cm, the height of the breeding water tank is 30cm, the water temperature in the water tank is controlled at 17°C, and the water level in the water tank is controlled at 1cm ;
[0023] S2: For seedling cultivation of Japanese leech, select Japanese leech seedlings with a weight of 0.5g / piece, and put the selected Japanese leech seedlings into the breeding tank at a density of 1000-1200 / square meter;
[0024] S3: Feed preparation, take the bait without adding anticoagulant and preservative, filter the bait with a 120-150 mesh filter, take the filtered plasma, and then put the plasma into artificial casing or pig small intestine , use cotton thread to tie up each section according to the leng...
Embodiment 2
[0034] A culture method for rapidly increasing the antithrombin activity of Japanese medicine leech is carried out according to the following steps:
[0035] S1: The setting of the breeding water tank, the length of the breeding water tank is 200cm, the width of the breeding water tank is 60cm, the height of the breeding water tank is 40cm, the water temperature in the water tank is controlled at 18°C, and the water level in the water tank is controlled at 3cm ;
[0036] S2: For seedling cultivation of Japanese leech, select Japanese leech seedlings with a weight of 0.5g / piece, and put the selected Japanese leech seedlings into the breeding tank at a density of 1000-1200 / square meter;
[0037] S3: Feed preparation, take the bait without adding anticoagulant and preservative, filter the bait with a 120-150 mesh filter, take the filtered plasma, and then put the plasma into artificial casing or pig small intestine , use cotton thread to tie up each section according to the leng...
Embodiment 3
[0047] A culture method for rapidly increasing the antithrombin activity of Japanese medicine leech is carried out according to the following steps:
[0048] S1: The setting of the breeding water tank, the length of the breeding water tank is 150cm, the width of the breeding water tank is 50cm, the height of the breeding water tank is 35cm, the water temperature in the water tank is controlled at 19°C, and the water level in the water tank is controlled at 2cm ;
[0049] S2: For seedling cultivation of Japanese leech, select Japanese leech seedlings with a weight of 0.5g / piece, and put the selected Japanese leech seedlings into the breeding tank at a density of 1000-1200 / square meter;
[0050] S3: Feed preparation, take the bait without adding anticoagulant and preservative, filter the bait with a 120-150 mesh filter, take the filtered plasma, and then put the plasma into artificial casing or pig small intestine , use cotton thread to tie up each section according to the leng...
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