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Method for extracting hirudin by stimulating living leech with electricity

A hirudin and electrical stimulation technology is applied in the preparation method of peptides, from leech inhibitors, chemical instruments and methods, etc., and can solve the problems of unsuccessful realization, affecting the purity of hirudin, and excessive dependence on the supply of leech resources.

Inactive Publication Date: 2011-08-10
南宁乙翔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the methods for industrial production of hirudin are to obtain hirudin by destroying the living leech, which affects the purity of the obtained hirudin. At the same time, it is too dependent on the supply of leech resources, and it is especially difficult to avoid the seasonal problem of hirudin production.
Utilize the stimulating effect of electric current on the glands of organisms, directly use DC weak current to stimulate living leeches to extract high-purity hirudin in batches and maintain the living traits of leeches in order to simplify the production process of hirudin, improve product purity, and maintain wild leeches The method of reusing resources, reducing production costs, and overcoming seasonal obstacles in production has not yet been successfully realized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] At room temperature of 20-30°C, the live leech is first taken from the breeding tank to the storage tank, rinsed with deionized water for 2 minutes to remove impurities on the surface of the live leech, filtered out the water, and then immersed in normal saline for 20 seconds to make the leech The surface is covered with electrolyte, the salt water is filtered out, and placed into a 200-mesh rectangular stainless steel filter tank. The filter tank is insulated from the ground. Under the filter screen is a sterilized and clean vessel of the same area. The two ends of the filter tank are respectively connected to the two poles of direct current. to the minimum output current, then turn on the power switch, and adjust the current to 1-6mA. After 40 minutes, disconnect the power supply, collect the liquid dripping from the filter tank in the vessel, and then put the leeches from the filter tank back into the storage tank, and then put them back into the storage tank. Return ...

Embodiment 2

[0022] At room temperature of 20-30°C, first take the living leech from the breeding tank to the storage tank, rinse with deionized water for 2 minutes to remove impurities on the surface of the living leech, filter out the water, and then immerse in normal saline and rinse for 20 seconds to make the surface of the leech Cover the electrolyte, filter out the brine, and put it into a rectangular stainless steel filter tank with a 200-mesh filter at the bottom. The filter tank is insulated from the ground. Under the filter screen is a sterilized and clean vessel of the same area. The two ends of the filter tank are respectively connected to the two poles of direct current. , first adjust to the minimum output current, turn on the power switch, and adjust the current to 5-12mA. After 20 minutes of electrical stimulation, disconnect the power supply, collect the liquid dripping from the filter tank in the vessel, and then put the leech back from the filter tank. storage tank, and t...

Embodiment 3

[0025] At a room temperature of 20-30°C, first take the live leech from the breeding tank to the storage tank, rinse with deionized water for 2 minutes to remove impurities on the surface of the live leech, filter out the water, and then immerse in normal saline and rinse for 20 seconds to cover the surface of the leech. Electrolyte, filter out salt water, put it flat into a rectangular stainless steel filter tank with a 200-mesh filter at the bottom. The filter tank is insulated from the ground. Under the filter screen is a sterilized and clean vessel of the same area. The two ends of the filter tank are respectively connected to the two poles of direct current. First adjust to the minimum output current, turn on the power switch, and adjust the current to 12-20mA. After 10 minutes of electrical stimulation, disconnect the power supply, collect the liquid dripping from the filter tank in the vessel, and then put the leech back from the filter tank for storage. trough, and put ...

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Abstract

The invention relates to a method for extracting hirudin by stimulating a living leech with electricity. By utilizing the simulating effect of current on organism glands, a way for simulating a living leech to extract high-purity hirudin at mass by direct weak current is directly used, and the living character of the leech is maintained, therefore, the production process of hirudin is simplified,the product purity is improved, the wild leech resources are recycled, the production cost is reduced and the problem of seasonal disorder of production is solved. The hirudin extracted by using the method is subjected to nanofiltration water removal and decompression spray drying to be made into powdery hirudin with the antithrombin activity of 800AT-U / g and liquid hirudin with the antithrombin activity of 1,000AT-U / g when protective agents are added in the liquid hirudin.

Description

technical field [0001] The invention relates to a method for extracting hirudin by electric stimulation of living leeches. Stimulating living leeches with weak electricity can cause the glands of the leeches to release glandular fluid, and at the same time ensure that the leeches remain in a living state, thereby achieving high yield from the glandular fluid of living leeches for many times. Methods of Purity Hirudin. Background technique [0002] Hirudin got its name because it was first isolated from leeches. Natural hirudin consists of 65 amino acid residues with a molecular weight of 7000 Daltons. Hirudin can irreversibly combine with the catalytic center of thrombin and the binding center of fibrinogen to inactivate thrombin. It is the most powerful thrombin inhibitor found so far. In addition, it has a strong antithrombotic effect, and has inhibitory effects on various experimental thrombus models. Hirudin has preventive and therapeutic effects on various thrombotic...

Claims

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Application Information

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IPC IPC(8): C07K14/815C07K1/14
Inventor 苏国强刁建中
Owner 南宁乙翔生物科技有限公司
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