Test box and detection method for rapidly detecting activity of antithrombin of leeches

An antithrombin and detection method technology, applied in the field of medical detection, can solve the problems of inconvenient portability and rapid detection, affecting procurement and collection cycles, and inability to perform rapid detection, and achieve the effects of light weight, low cost, and rapid detection

Inactive Publication Date: 2018-12-11
CHONGQING DUOPUTAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] According to the requirements of the 2015 Pharmacopoeia, the conditions for the determination of leech antithrombin activity are relatively harsh. It needs to be tested in a water bath at 36±0.5°C, and the test equipment is relatively large, which is inconvenient to carry and quickly detect. However, most leeches are wild at present. When measuring the antithrombin activity of wild leeches, it needs to be collected and mailed back to the laboratory for the determination of antithrombin activity, which cannot be quickly determined on site, which affects the procurement and collection cycle

Method used

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  • Test box and detection method for rapidly detecting activity of antithrombin of leeches
  • Test box and detection method for rapidly detecting activity of antithrombin of leeches
  • Test box and detection method for rapidly detecting activity of antithrombin of leeches

Examples

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Embodiment 1

[0029] In May 2018, in Jiulong Town, Guang'an City, Sichuan Province, some wild Japanese medical leeches were captured in the wild, and the test box for rapid detection of leech antithrombin activity of the present invention was used for detection in the field. In the test box, there are 5 10ml graduated centrifuge tubes, 5ml of 0.9% sodium chloride solution inside, 5 test tubes (9×110mm), 1 bottle of prepared thrombin solution; 1 bottle of prepared 0.5% fibrinogen Tris hydrochloric acid buffer; 1 scissors; 2 beakers; 1 percent electronic balance; 1 500ul sampler; 2 100ul samplers, 1 thermometer.

[0030] Randomly select six captured Japanese medical leeches, divide them into two groups, respectively, test group 1-1 and test group 1-2, place the two groups of leeches in two burnouts, cut them into pieces, and test the leech resistance Thrombin activity.

[0031] Wherein the test group 1-1 is treated as follows:

[0032] 1) Take the leech whose weight M is 1g and cut it into ...

Embodiment 2

[0045] In January 2018, in Luchuan County, Yulin City, Guangxi, a number of wild wide-bodied golden leeches were captured in the wild, and the test box for rapid detection of leech antithrombin activity of the present invention was used for detection in the field. In the test box, there are 5 10ml graduated centrifuge tubes, 5ml of 0.9% sodium chloride solution inside, 5 test tubes (9×110mm), 1 bottle of prepared thrombin solution; 1 bottle of prepared 0.5% fibrinogen Tris hydrochloric acid buffer; 1 scissors; 2 beakers; 1 percent electronic balance; 1 500ul sampler; 2 100ul samplers, 1 thermometer.

[0046] Randomly select six captured wide-bodied golden leeches, and divide them into two groups, respectively, test group 2-1 and test group 2-2, place the two groups of leeches in two burnouts, cut them into pieces, and test them separately Leech antithrombin activity.

[0047] Wherein the test group 2-1 is treated as follows:

[0048] 1) Take the leech whose weight M is 1g an...

Embodiment 3

[0061] In July 2018, in Changtan Town, Wanzhou District, Chongqing City, a number of wild wide-bodied golden leeches were captured in the wild, and the test box for rapid detection of leech antithrombin activity of the present invention was used for detection in the field. In the test box, there are 5 10ml graduated centrifuge tubes, 5ml of 0.9% sodium chloride solution inside, 5 test tubes (9×110mm), 1 bottle of prepared thrombin solution; 1 bottle of prepared 0.5% fibrinogen Tris hydrochloric acid buffer; 1 scissors; 2 beakers; 1 percent electronic balance; 1 500ul sampler; 2 100ul samplers, 1 thermometer.

[0062] Randomly select six captured wide-bodied golden leeches, and divide them into two groups, respectively, test group 3-1 and test group 3-2, place the two groups of leeches in two burnouts, cut them into pieces, and test them respectively Leech antithrombin activity.

[0063] Wherein test group 3-1 is processed as follows:

[0064] 1) Take the leech whose weight M...

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Abstract

The invention discloses a test box for rapidly detecting the activity of antithrombin of leeches. The test box comprises a 0.9 percent sodium chloride solution, a trihydroxymethyl aminomethane hydrochloric acid buffering solution containing 0.5 percent fibrinogen and a prepared thrombin solution. The invention further discloses a detection method for rapidly detecting the activity of the antithrombin of the leeches; the detection method comprises the following steps: 1) weighing and chopping the leaches and putting the leaches into the sodium chloride solution; 2) extracting and centrifuging;3) adding the trihydroxymethyl aminomethane hydrochloric acid buffering solution; 4) dropwise adding the prepared thrombin solution; 5) calculating; 6) calibrating according to environment temperature. The test box and the detection method can be used for rapidly detecting the activity of the antithrombin in the leeches, have the advantages of accurate detection result, low equipment requirementsand rapid detection, and can be suitable for outdoor on-site operation.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a test box and a detection method for rapidly detecting leech antithrombin activity. Background technique [0002] According to the requirements of the 2015 Pharmacopoeia, the conditions for the determination of leech antithrombin activity are relatively harsh. It needs to be tested in a water bath at 36±0.5°C, and the test equipment is relatively large, which is inconvenient to carry and quickly detect. However, most leeches are wild at present. When measuring the antithrombin activity of wild leeches, it needs to be collected and mailed back to the laboratory for the determination of antithrombin activity. It cannot be quickly determined on site, which affects the procurement and collection cycle. [0003] Therefore those skilled in the art are committed to developing a test box and a rapid detection method that can rapidly detect antithrombin activity in fresh and li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573
Inventor 万芳娟颜世平甘奇超郭平牯
Owner CHONGQING DUOPUTAI PHARMA
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