Breeding method for fast increasing activity of antithrombase of leeches
An antithrombin and breeding method technology, which is applied in the breeding field of rapidly increasing the antithrombin activity of leech, can solve the problems of low efficiency of antithrombin activity, large body weight loss, long breeding cycle and the like, and achieves the breeding cycle. The effect of shortening, reducing disease cross-infection, and reducing production management costs
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Embodiment 1
[0061] A culture method for rapidly increasing the antithrombin activity of leeches, comprising the following steps: (1) seedling collection: collect the leech seedlings after hatching, and carry out separate seedling treatment after 20 days of concentrated seedling cultivation; They were fed daily at 22°C for 2 months, and gained weight rapidly; the breeding density was 60 fish / L, and the bait (fresh bovine serum) was fed once every 4 days with the bionic membrane as the medium, and the feeding time was selected at 12 am. Before: 00, leech is cleaned up before throwing something to feed, and feeds after draining water, and eating time is controlled at 30min; During leech feeding process, pick out the oversized individual at any time, to control its single food intake, prevent swelling and death; ( 3) Temperature control and fasting: the breeding temperature was controlled at 30° C., and the constant temperature was fasted for 1 month; (4) Harvesting. In addition, in the breed...
Embodiment 2
[0063] A culture method for rapidly increasing the antithrombin activity of leeches, comprising the following steps: (1) seedling collection: collect the leech seedlings after hatching, and carry out separate seedling treatment after 20 days of concentrated seedling cultivation; They were fed daily at 34°C for 2 months, and gained weight rapidly; the breeding density was 80 pigs / L, and the bait (fresh pig serum) was fed once every 4 days with the bionic membrane as the medium, and the feeding time was selected at 12 am. Before: 00:00, the leeches were cleaned before feeding, and the leeches were fed after draining the water, and the feeding time was controlled at 90 minutes; (3) temperature control and fasting: the feeding temperature was controlled at 30 ° C, and the constant temperature was fasted for 1 month; (4) Harvest . In addition, in the breeding process of steps (2) and (3), it is also necessary to clean and change water, that is, to wash and change once a day with cl...
Embodiment 3
[0065] A culture method for rapidly increasing the antithrombin activity of leeches, comprising the following steps: (1) seedling collection: collect the leech seedlings after hatching, and carry out separate seedling treatment after 20 days of concentrated seedling cultivation; (2) weight gain culture: add about 1 g The leech seedlings were fed daily at 30°C for 2 months, and gained weight rapidly; the breeding density was 75 tails / L, and the bait (celery juice with a volume ratio of 2:8) was fed once every 4 days with the bionic film as the medium. and bovine serum), the feeding time is selected before 12:00 in the morning, the leeches are cleaned before feeding, the water is filtered and fed, and the feeding time is controlled at 60 minutes; the leeches are picked out at any time during the feeding process. In order to control its single food intake and prevent bloating; after feeding, separate the leech from the feeding container, clean the leech and put it back in the feed...
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