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Thrombin Chromogenic Substrate Solution, Thrombin Aqueous Solution, Method and Kit for Antithrombin III Activity Determination

A chromogenic substrate, antithrombin technology, applied in the direction of chemical reaction of materials, material analysis by observing the impact on chemical indicators, etc., can solve the problems to be improved, and achieve consistency between bottles Good performance, reduce production costs, and avoid the effect of result deviation

Active Publication Date: 2020-08-04
SHANGHAI LONG ISLAND BIOTEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current chromogenic substrate method for detecting antithrombin III activity still needs to be improved

Method used

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  • Thrombin Chromogenic Substrate Solution, Thrombin Aqueous Solution, Method and Kit for Antithrombin III Activity Determination
  • Thrombin Chromogenic Substrate Solution, Thrombin Aqueous Solution, Method and Kit for Antithrombin III Activity Determination
  • Thrombin Chromogenic Substrate Solution, Thrombin Aqueous Solution, Method and Kit for Antithrombin III Activity Determination

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Experimental program
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Effect test

preparation example Construction

[0032] The preparation method of the thrombin aqueous solution is not particularly limited as long as predetermined components exist in the finally obtained aqueous solution.

[0033] According to specific examples of the present invention, thrombin aqueous solution can be prepared through the following steps:

[0034] First, dissolve tris(hydroxymethyl)aminomethane (Tris), heparin sodium, gelatin, amino acids, bovine serum albumin (BSA), polyethylene glycol, sucrose, inorganic salts, and sodium azide in water to obtain thrombin protection solution, adjust the pH value to 7.0-9.0.

[0035] Next, the thrombin freeze-dried powder is dissolved in the obtained thrombin protection solution to obtain a thrombin aqueous solution, wherein the thrombin enzyme activity in the thrombin aqueous solution can be 10000-15000 IU / L.

[0036] In addition, thrombin aqueous solution can also be prepared by the following steps:

[0037] First, take the freeze-dried thrombin powder and dissolve i...

Embodiment 1

[0062] Embodiment 1 prepares AT-III activity assay reagent R1 (thrombin aqueous solution)

[0063] According to the steps of the general method, and according to the components and final concentrations listed in the table below, prepare AT-III activity assay reagent thrombin aqueous solution R1-1, R1-2, R1-3, R1-4, R1-5 , R1-6, R1-7 and R1-8, wherein R1-8 is used as a reference substance.

[0064] Table 1 Composition of Reagent R1

[0065]

[0066]

Embodiment 2

[0067] Example 2 Preparation of AT-III Determination Reagent R2 (Thrombin Chromogenic Substrate Solution)

[0068] Prepare AT-III activity assay reagent thrombin chromogenic substrate solution R2-1, R2-2, R2-3, R2-4 according to the steps of the general method, and according to the components and final concentrations listed in the table below , R2-5, R2-6, R2-7 and R2-8, wherein R2-8 is used as a reference substance.

[0069] The composition of table 2 reagent R2

[0070]

[0071]

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Abstract

The invention relates to a thrombin chromogenic substrate solution for determination of antithrombin III activity, a thrombin aqueous solution for determination of antithrombin III activity, a method for determination of antithrombin III activity in samples, a kit for determination of antithrombin III activity, application of sorbitol in preparation of the thrombin chromogenic substrate solution, and application of cane sugar in the chromogenic substrate method for determination of antithrombin III activity. The thrombin chromogenic substrate solution for determination of antithrombin III activity contains the thrombin chromogenic substrate, sorbitol and water. The thrombin chromogenic substrate solution can effectively be used for determination of antithrombin III activity.

Description

technical field [0001] The present invention relates to the field of biochemical detection. Specifically, the present invention relates to a thrombin chromogenic substrate liquid, an aqueous solution of thrombin, a method and a kit for the determination of antithrombin III activity. More specifically, the present invention relates to a A thrombin chromogenic substrate solution for the determination of antithrombin III activity, an aqueous solution of thrombin for the determination of antithrombin III activity, a method for determining the activity of antithrombin III in a sample, a A kit for the determination of antithrombin III activity, the use of sorbitol in the preparation of thrombin chromogenic substrate solution and the use of sucrose in the determination of antithrombin III activity by chromogenic substrate method. Background technique [0002] Antithrombin III (antithrombin III, AT-III) is the most important anticoagulant in plasma physiological inhibitors. The AT-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 艾峰朱斌斌
Owner SHANGHAI LONG ISLAND BIOTEC CO LTD
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