Freeze dried powder injection of hirudin and its preparation method

Abstract

The invention discloses a freeze dry injection of hirudin and its preparation process. It is characterized in that hirudin is extracted from fresh leech, pharmaceutic adjuvant can be added or not added after purification to prepare freeze dry injection formulation of hirudin, the character also lies in not adopting any organic solvent in the preparation method, but adopting purification methods of ultra filtration, ion exchange column chromatography and reverse osmosis condensation to get hirudin with comparative highly purity. The pharmacody experiment result indicates that the freeze dry injection has a favorable pharmacological action.

Description

Technical field

[0001] The invention belongs to the technical field of traditional Chinese medicine pharmacy, and in particular relates to a hirudin freeze-dried powder injection and a preparation method thereof. Background technique

[0002] Leech is a traditional Chinese medicine in my country, which was recorded in "Shen Nong's Materia Medica" 1800 years ago. Traditional Chinese medicine believes that it has the effects of breaking blood, expelling blood stasis, and dredging menstrual flow. It is mainly used to treat lumps, lumps, blood stasis, amenorrhea, and bruises. In the West, leeches are also commonly used to suck blood to treat certain diseases. A variety of active ingredients have been extracted from leeches and their salivary glands, among which hirudin is the most active and most researched ingredient. It is a small molecular protein (polypeptide) composed of 65-66 amino acids. Hirudin has a strong inhibitory effect on thrombin, and is the strongest natural sp...

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