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Silk fibroin anticoagulant material and preparation method thereof

A silk fibroin and anticoagulant technology, applied in anticoagulant treatment, packaging item types, special packaging items, etc., can solve problems such as reducing the activity of hirudin, and prevent neointimal hyperplasia and postoperative thrombosis. , the effect of inhibiting thrombin activity

Active Publication Date: 2017-05-31
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

We have researched and developed a hirudin / silk fibroin anticoagulant material (an anticoagulant silk fibroin material and its preparation method, the Chinese patent application number is ZL201310250951.X; an anticoagulant silk fibroin film and its preparation method , Chinese patent with application number ZL201310251819.0; Journal of Biomedical Materials Research Part B, 2015:103B:556–562), but in-depth research found that the research results were combined with the mechanism of hirudin’s inhibition of thrombin activity. The modification method of valence bond will reduce the activity of hirudin

Method used

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preparation example Construction

[0025] The present invention also provides a preparation method of the above-mentioned silk fibroin anticoagulant material, comprising the following steps: S1) mixing the silk fibroin solution, polyethylene glycol diamine and a cross-linking agent, dialyzing after the reaction, and drying to obtain Polyethylene glycol diamine cationized silk fibroin material; S2) The polyethylene glycol diamine cationized silk fibroin material was soaked in a hirudin solution to obtain a silk fibroin anticoagulant material.

[0026] The present invention has no special limitation on the sources of all raw materials, which can be commercially available or self-made.

[0027] Wherein, the mass concentration of silk fibroin in the silk fibroin solution is preferably 3% to 20%; the silk fibroin can be silk fibroin well-known to those skilled in the art, and there is no special limitation. It is preferably silkworm silk fibroin; the silk fibroin solution is preferably prepared according to the foll...

Embodiment 1

[0041] 1.1 Put silkworm silk or cocoon shells dried and stratified into 0.2% aqueous sodium carbonate solution at a bath ratio of 1:50 (g / mL), and treat them three times at 98°C to 100°C, each time for 30 minutes , and then fully wash the silk with deionized water, loosen it, and place it in an oven at 60° C. to dry to obtain degummed silkworm silk fiber.

[0042] 1.2 Weigh the silkworm silk fibroin after degumming and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 (the molar ratio of ethanol to water is 1:4) at a bath ratio of 1:10 (g / mL), 70 ℃ for 2 hours to obtain silkworm fibroin solution.

[0043] 1.3 Pour the silkworm fibroin solution into the dialysis bag. The wall of the dialysis bag is a semi-permeable membrane with a molecular weight cut-off of 12.0-16.0kDa. Place the dialysis bag filled with the silkworm silk solution into a container filled with deionized water Inside, the water in the container was replaced with new deion...

Embodiment 2

[0048] 2.1 Put silkworm silk or cocoon shells dried and stratified into 0.2% aqueous sodium carbonate solution at a bath ratio of 1:50 (g / mL), and treat them three times at 98°C to 100°C, each time for 30 minutes , and then fully wash the silk with deionized water, loosen it, and place it in an oven at 60° C. to dry to obtain degummed silkworm silk fiber.

[0049] 2.2 Weigh the degummed silkworm silk fibroin and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 (the molar ratio of ethanol to water is 1:4) at a bath ratio of 1:10 (g / mL), 70 ℃ for 2 hours to obtain silkworm fibroin solution.

[0050] 2.3 Pour the silkworm fibroin solution into the dialysis bag. The wall of the dialysis bag is a semi-permeable membrane with a molecular weight cut-off of 12.0-16.0kDa. Place the dialysis bag filled with the silkworm silk solution into a container filled with deionized water Inside, the water in the container was replaced with new deionized wa...

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Abstract

The invention provides a preparation method of a silk fibroin anticoagulant material. The preparation method comprises the following steps: (S1) mixing a silk fibroin solution and polyethylene glycol diamine with a cross-linking agent for carrying out a reaction, then carrying out dialysis, and drying to obtain a polyethylene glycol diamine cationized silk fibroin material; (S2) dipping the polyethylene glycol diamine cationized silk fibroin material into a hirudin solution to obtain the silk fibroin anticoagulant material. Compared with the prior art, the preparation method provided by the invention protects a functional group at a thrombin bonding region from being affected by the reaction so as to prevent the influence on a structural domain bonded with the thrombin, and also can enable hirudin to be bonded onto the polyethylene glycol diamine cationized silk fibroin by means of an ionic bond with a stronger bonding force, thus achieving the effect of stably giving play to anticoagulation; therefore, the obtained silk fibroin anticoagulant material has an obvious function of inhibiting the activity of the thrombin, and is especially applied to prevention of the neointimal hyperplasia and postoperative thrombus formation of artificial blood vessels.

Description

technical field [0001] The invention belongs to the technical field of anticoagulant materials, and in particular relates to a silk fibroin anticoagulant material and a preparation method thereof. Background technique [0002] There are millions of deaths from cardiovascular and cerebrovascular diseases in my country every year, and it is increasing year by year. Therefore, blood-contact materials are currently the most scarce biomaterials (medical devices) in clinical practice, especially artificial blood vessel grafts. , Large-diameter artificial blood vessels are also very rare in my country, and the annual use ratio of domestic products is only about 20%, while the transplantation of small-caliber artificial blood vessels is still a clinical blank. The biggest problem is that it is easy to form thrombus, and the long-term patency rate is poor . [0003] At present, artificial blood vessels used in medicine are mainly made of synthetic materials such as polyester and expan...

Claims

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Application Information

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IPC IPC(8): A61L33/12A61L33/06A61L33/14
CPCA61L33/0023A61L33/0076A61L33/068A61L33/128C08L89/00C08L71/02
Inventor 王建南石盼格李荷蕾裔洪根
Owner SUZHOU UNIV
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