Cationic silk fibroin material, its preparation method and application

A technology of silk fibroin and cationization, applied in the field of cationized silk fibroin material, can solve the problem of reducing the activity of hirudin, and achieve the effect of inhibiting the activity of thrombin

Active Publication Date: 2020-05-22
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

We have researched and developed a hirudin / silk fibroin anticoagulant material (an anticoagulant silk fibroin material and its preparation method, the Chinese patent application number is ZL201310250951.X; an anticoagulant silk fibroin film and its preparation method , Chinese patent with application number ZL201310251819.0; Journal of Biomedical Materials Research Part B, 2015:103B:556–562), but in-depth research found that the research results were combined with the mechanism of hirudin’s inhibition of thrombin activity. The modification method of valence bond will reduce the activity of hirudin

Method used

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preparation example Construction

[0027] The present invention also provides a method for preparing the above-mentioned cationized silk fibroin material, comprising the following steps: mixing the silk fibroin solution, polyethylene glycol diamine and a cross-linking agent, and dialyzing after the reaction to obtain the cationized silk fibroin Material.

[0028] The present invention has no special limitation on the sources of all raw materials, which can be commercially available or self-made.

[0029] Wherein, the mass concentration of silk fibroin in the silk fibroin solution is preferably 3% to 20%; the silk fibroin can be silk fibroin well-known to those skilled in the art, and there is no special limitation. It is preferably silkworm silk fibroin; the silk fibroin solution is preferably prepared according to the following method: degumming and dissolving silk or silkworm shells, pouring them into a dialysis bag, and dialysis with deionized water to obtain a silk fibroin solution.

[0030] Wherein, the d...

Embodiment 1

[0046] 1.1 Put silkworm silk or cocoon shells dried and stratified into 0.2% aqueous sodium carbonate solution at a bath ratio of 1:50 (g / mL), and treat them three times at 98°C to 100°C, each time for 30 minutes , and then fully wash the silk with deionized water, loosen it, and place it in an oven at 60° C. to dry to obtain degummed silkworm silk fiber.

[0047] 1.2 Weigh the silkworm silk fibroin after degumming and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 (the molar ratio of ethanol to water is 1:4) at a bath ratio of 1:10 (g / mL), 70 ℃ for 2 hours to obtain silkworm fibroin solution.

[0048] 1.3 Pour the silkworm fibroin solution into the dialysis bag. The wall of the dialysis bag is a semi-permeable membrane with a molecular weight cut-off of 12.0-16.0kDa. Place the dialysis bag filled with the silkworm silk solution into a container filled with deionized water Inside, the water in the container was replaced with new deion...

Embodiment 2

[0052] 2.1 Put silkworm silk or cocoon shells dried and stratified into 0.2% aqueous sodium carbonate solution at a bath ratio of 1:50 (g / mL), and treat them three times at 98°C to 100°C, each time for 30 minutes , and then fully wash the silk with deionized water, loosen it, and place it in an oven at 60° C. to dry to obtain degummed silkworm silk fiber.

[0053] 2.2 Weigh the degummed silkworm silk fibroin and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 (the molar ratio of ethanol to water is 1:4) at a bath ratio of 1:10 (g / mL), 70 ℃ for 2 hours to obtain silkworm fibroin solution.

[0054] 2.3 Pour the silkworm fibroin solution into the dialysis bag. The wall of the dialysis bag is a semi-permeable membrane with a molecular weight cut-off of 12.0-16.0kDa. Place the dialysis bag filled with the silkworm silk solution into a container filled with deionized water Inside, the water in the container was replaced with new deionized wa...

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Abstract

The invention provides a preparation method of a cationic silk fibroin material. The method comprises the steps as follows: a silk fibroin solution, polyethylene bis(amine) and a crosslinking agent are mixed, dialysis is performed after the reaction, and the cationic silk fibroin material is obtained. Compared with the prior art, the prepared cationic silk fibroin material can be mixed with hirudin, particularly mixed with hirudin after dialysis, for preparation of an anticoagulation material, the structural domain bond with thrombin is protected against influences of reacted functional groups (-COOH, -NH2 and -OH) in a thrombin binding area, hirudin can be bond to polyethylene bis(amine) cationic silk fibroin through ionic bonds with strong binding force, and part of hirudin is coated with silk fibroin and is gradually released along with degradation of the silk fibroin, so that the anticoagulation effect is played stably and lastingly while the obtained anticoagulation material has a significant thrombin activity inhibition function.

Description

technical field [0001] The invention belongs to the technical field of anticoagulation materials, and in particular relates to a cationic silk fibroin material, its preparation method and application. Background technique [0002] There are millions of deaths from cardiovascular and cerebrovascular diseases in my country every year, and it is increasing year by year. Therefore, blood-contact materials are currently the most scarce biomaterials (medical devices) in clinical practice, especially artificial blood vessel grafts. , Large-diameter artificial blood vessels are also very rare in my country, and the annual use ratio of domestic products is only about 20%, while the transplantation of small-caliber artificial blood vessels is still a clinical blank. The biggest problem is that it is easy to form thrombus, and the long-term patency rate is poor . [0003] At present, artificial blood vessels used in medicine are mainly made of synthetic materials such as polyester and e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L33/12A61L33/06A61L33/14
CPCA61L33/0041A61L33/0076A61L33/062A61L2300/252A61L2300/42A61L2300/602C08L89/00C08L71/02
Inventor 王建南李荷蕾王琼玉裔洪根
Owner SUZHOU UNIV
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