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Anticoagulation fibroin material and preparation method

A technology for coagulation silk and silk fibroin, which is applied in the field of anticoagulant materials and its preparation, can solve the problems of unfavorable endothelialization, poor long-term patency rate, influence on tissue healing, etc., and achieve the effect of lasting anticoagulation effect.

Active Publication Date: 2013-09-11
常熟紫金知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, artificial blood vessels currently used in medicine are mainly made of synthetic materials such as polyester and expanded polytetrafluoroethylene, which have great applications in the development of large and medium-caliber artificial blood vessels, but these synthetic materials have poor cytocompatibility. It is not conducive to endothelialization, easy to form thrombus, poor long-term patency rate, and affects tissue healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: The preparation method of a kind of anticoagulant thrombin material is as follows, put the cocoon shell of silkworm raw silk or drying stratification into the sodium carbonate aqueous solution that concentration is 0.2% by the bath ratio of 1:50 (g / mL) , soaked at 98-100°C for three times, each time for 30 minutes, then thoroughly cleaned silkworm silk or cocoon shells with deionized water and loosened them, then dried them in an oven at 60°C to obtain degummed silkworm silk fibroin Fiber: Weigh the degummed silkworm silk fiber and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 at a bath ratio of 1:10 (g / mL), and dissolve it at 70°C for 2 hours to obtain silkworm silk fibroin dissolution solution; then the silkworm fibroin solution was poured into the dialysis bag, the wall of the dialysis bag was made of semi-permeable membrane, and the molecular weight cut-off was in the range of 12.0-16.0 kDa, and the dialysis ...

Embodiment 2

[0027] Embodiment 2: A preparation method of an anticoagulant silk fibroin material, adopting the method in Embodiment 1 to prepare a silk fibroin solution with a concentration of 8%, adding N-hydroxysuccinimide to the silk fibroin solution, The mass ratio of the N-hydroxysuccinimide and the silk fibroin in the silk fibroin solution is 1:10. After stirring evenly, place it for 10 minutes, then add hirudin, and the hirudin and the silk fibroin in the silk fibroin solution The mass ratio of the mixture is 0.5:10000, mix it evenly and pour it into a flat polystyrene plate, and place the polystyrene plate in a fume hood to dry and solidify, take it out and place it in a container filled with 80% ethanol for 2 hour, then take it out and put it into a container filled with deionized water and soak for 3 days, change the deionized water in the container every 2 hours, take it out and air-dry after 3 days, and obtain the anticoagulant fibroin material.

[0028] Cut the prepared an...

Embodiment 3

[0030] Embodiment 3: A preparation method of an anticoagulant silk fibroin material, using the method in Example 1 to prepare a silk fibroin solution with a concentration of 8%, adding N-hydroxysuccinimide to the above silk fibroin solution, The mass ratio of the N-hydroxysuccinimide and the silk fibroin in the silk fibroin solution is 1:10. After stirring evenly, place it for 10 minutes, then add hirudin, and the hirudin and the silk fibroin in the silk fibroin solution The mass ratio is 2:10000, mix it evenly and pour it into a flat polystyrene plate, freeze it at -80℃~-20℃ for 2~24 hours, then dry it, take it out and put it in a container filled with 80% ethanol Immerse in water for 2 hours, then take it out and put it in a container filled with deionized water to soak for 3 days, replace the deionized water in the container every 2 hours, take it out and air dry after 3 days, and obtain the anticoagulant fibroin material.

[0031] Cut the prepared anticoagulant fibroin...

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PUM

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Abstract

The invention discloses an anticoagulation fibroin material, which comprises components such as fibroin protein and hirudin. An anticoagulation fibroin material preparation method comprises the following steps: (1) preparing a fibroin protein solution with a concentration of 4-20%; (2) dissolving N-hydroxy succinimide in the fibroin protein solution in the step (1), uniformly stirring, standing, adding hirudin, and uniformly stirring to obtain a mixed solution; and (3) curing the mixed solution obtained from the step (2), adopting a methanol solution or an ethanol solution to carry out a dipping treatment, then soaking in deionized water, taking the obtained material, and carrying out air drying on the material to obtain the anticoagulation fibroin material. Compared with the fibroin material with no hirudin addition, the anticoagulation fibroin material of the present invention has the following advantages that: an anticoagulation performance is significantly increased, and hirudin in the anticoagulation fibroin material and activated fibroin protein can be directly subjected to stable covalent bonding through an amide bond so as to lastingly provide an anticoagulation effect.

Description

technical field [0001] The invention relates to an anticoagulant material and a preparation method thereof, in particular to an anticoagulant thrombin material and a preparation method thereof. Background technique [0002] Cardiovascular disease has become the number one killer of human beings, seriously threatening human health. Materials in contact with blood, such as artificial blood vessels, are currently the most urgently needed biomedical materials and clinical materials. For example, artificial blood vessels currently used in medicine are mainly made of synthetic materials such as polyester and expanded polytetrafluoroethylene, which have great applications in the development of large and medium-caliber artificial blood vessels, but these synthetic materials have poor cytocompatibility. It is not conducive to endothelialization, easy to form thrombus, and the long-term patency rate is poor, which affects tissue healing. Therefore, it is necessary to develop an arti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L33/12A61L27/22A61L31/04
Inventor 孙丹魏雅丽裔洪根王建南
Owner 常熟紫金知识产权服务有限公司
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