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Anticoagulation fibroin membrane and preparation method thereof

A technology for coagulation silk and silk fibroin, which is applied in the field of silk fibroin membrane and its preparation, and can solve the problems of easy thrombus formation, unfavorable endothelialization, and influence on tissue healing, etc.

Active Publication Date: 2015-03-04
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, artificial blood vessels currently used in medicine are mainly made of synthetic materials such as polyester and expanded polytetrafluoroethylene, which have great applications in the development of large and medium-caliber artificial blood vessels, but these synthetic materials have poor cytocompatibility. It is not conducive to endothelialization, easy to form thrombus, poor long-term patency rate, and affects tissue healing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one: the preparation method of a kind of anticoagulant thrombin film is as follows, put the cocoon shell of silkworm raw silk or drying stratification into the sodium carbonate aqueous solution that concentration is 0.2% by the bath ratio of 1:50 (g / mL) , soaked at 98-100°C for three times, each time for 30 minutes, then thoroughly cleaned silkworm silk or cocoon shells with deionized water and loosened them, then dried them in an oven at 60°C to obtain degummed silkworm silk fibroin Fiber: Weigh the degummed silkworm silk fiber and dissolve it in an aqueous solution of calcium chloride-ethanol with a molar ratio of 1:2 at a bath ratio of 1:10 (g / mL), and dissolve it at 70°C for 2 hours to obtain silkworm silk fibroin dissolution solution; then the silkworm fibroin solution was poured into the dialysis bag, the wall of the dialysis bag was made of semi-permeable membrane, and the molecular weight cut-off was in the range of 12.0-16.0 kDa, and the dialysis ba...

Embodiment 2

[0027] Example 2: A method for preparing an anticoagulant silk fibroin film, using the method in Example 1 to prepare a silk fibroin solution with a concentration of 8%, adding hirudin and adhesive polyethylene glycol to the above silk fibroin solution Alcohol diglycidyl ether, the mass ratio of hirudin, polyethylene glycol diglycidyl ether and silk fibroin in the silk fibroin solution is 0.5:10000 and 0.8:1 respectively, after stirring evenly, pour into a flat poly Place the polystyrene flat dish in a fume hood to air-dry and solidify, then take it out and put it in a container filled with deionized water to soak for 3 days, and replace the deionized water in the container every 2 hours, 3 Take it out and air-dry it 2 days later to obtain an anticoagulant fibroin film.

[0028] Cut the prepared anticoagulant fibroin film into small discs or small squares of appropriate size, measure the hemolytic performance of the anticoagulant fibroin film according to the hemolysis rat...

Embodiment 3

[0030] Example 3: A method for preparing an anticoagulant silk fibroin film, using the method in Example 1 to prepare a silk fibroin solution with a concentration of 8%, adding hirudin and an adhesive polyethylene glycol to the above silk fibroin solution Alcohol diglycidyl ether, the mass ratio of hirudin, polyethylene glycol diglycidyl ether to silk fibroin in the silk fibroin solution is 2:10000 and 0.8:1 respectively, after stirring evenly, pour into a flat poly In a styrene plate, freeze at -80°C~-20°C for 2~24 hours, take it out and thaw at room temperature, air dry, then put it in a container filled with deionized water for 3 days, every 2 hours The deionized water in the container was replaced once, and after 3 days, it was taken out and air-dried to obtain an anticoagulant fibroin film.

[0031] Cut the prepared anticoagulant fibroin film into small discs or small squares of appropriate size, measure the hemolytic performance of the anticoagulant fibroin film acco...

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PUM

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Abstract

The invention discloses an anticoagulation fibroin membrane. The anticoagulation fibroin membrane comprises silk fibroin, hirudin, and an adhesive. The preparation method of the anticoagulation fibroin membrane comprises the following steps of: (1) preparing a silk fibroin solution with the concentration of 4-20%; (2) adding the hirudin and the adhesive into the silk fibroin solution prepared in the step (1) and uniformly mixing to obtain a mixed solution; and (3) curing the mixed solution obtained in the step (2), soaking the cured product in deionized water, and at last taking the soaked product out and air-drying to obtain the anticoagulation fibroin membrane. Compared with the adhesive fibroin membrane without hirudin, the anticoagulation fibroin membrane comprising trace hirudin, disclosed by the invention, has the advantages that the anticoagulation performance is obviously improved, the hirudin and the silk fibroin can form a stable gel network through covalent bonding of amido bonds to exert a long-lasting anticoagulation effect.

Description

technical field [0001] The invention relates to a silk fibroin film and a preparation method thereof, in particular to an anticoagulant silk fibroin film and a preparation method thereof. Background technique [0002] Cardiovascular disease has become the number one killer of human beings, seriously threatening human health. Materials in contact with blood, such as artificial blood vessels, are currently the most urgently needed biomedical materials and clinical materials. For example, artificial blood vessels currently used in medicine are mainly made of synthetic materials such as polyester and expanded polytetrafluoroethylene, which have great applications in the development of large and medium-caliber artificial blood vessels, but these synthetic materials have poor cytocompatibility. It is not conducive to endothelialization, easy to form thrombus, and the long-term patency rate is poor, which affects tissue healing. Therefore, it is necessary to develop an artificial...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/54
Inventor 魏雅丽孙丹裔洪根王建南
Owner SUZHOU UNIV
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