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Large-scale production method for natural hirudin

A technology of natural hirudin and production method, which is applied in the preparation methods of peptides, chemical instruments and methods, and leech inhibitors, etc., to achieve the effects of promoting metabolism, improving blood circulation, and promoting growth

Active Publication Date: 2014-11-19
深圳金康道夫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In order to solve the problems in the above-mentioned prior art, the present invention will use an emetic to replace the artificial extrusion method to collect the natural hirudin crude product liquid, thereby overcoming the shortcomings existing in the multiple extractions of the blood-sucking leech living body, and then improving production efficiency, Reduce the mortality of blood-sucking leeches and reduce costs to realize the large-scale production of natural hirudin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A large-scale production method of natural hirudin, comprising the steps of:

[0050] 1. Preparation and feeding of inducing substances: Prepare and feed according to the method of Chinese invention patent: 03113566.8 invented by the inventor. Dissolve acetic acid, citric acid, sodium chloride, and sodium carbonate in distilled water to control the concentration at 0.1-0.5%, and the pH is about 4.5 to obtain the inducing substance. Then put it into the cleaned blood-sucking leech living body, let the blood-sucking leech fully absorb satiety, just can obtain the blood-sucking leech living body after absorbing the inducing substance, and set aside.

[0051] 2. Emetic induction: Take 52 kg of the blood-sucking leech living body after absorbing the satiety-inducing substance and put it in a container, then add 52 kg of 0.08% zinc sulfate solution, let it stand for 10 minutes, and wait for the blood-sucking leech living body to spit out the saliva in its body, that is, the n...

Embodiment 2

[0056] A large-scale production method of natural hirudin, comprising the steps of:

[0057] 1. Preparation and feeding of inducing substances: Prepare and feed according to the method of Chinese invention patent: 03113566.8 invented by the inventor. Use the aqueous solution of the mixture of arginine, cystine and sodium phosphate, control the concentration at 0.5-1%, and the pH at 4.5-6.8, and dissolve it in distilled water to obtain the inducing substance. Then put it into the cleaned blood-sucking leech living body, let the blood-sucking leech fully absorb satiety, just can obtain the blood-sucking leech living body after absorbing the inducing substance, and set aside.

[0058] 2. To induce vomiting:

[0059] (1) Prepare the emetic first: Mix 1.7 kg of 1.0% zinc sulfate solution with 1.7 kg of 10% ethanol to obtain the desired emetic and set aside.

[0060] (2) Emetic: Take 34 kg of the living blood-sucking leech after absorbing the satiety-inducing substance and put it ...

Embodiment 3

[0065] A large-scale production method of natural hirudin, comprising the steps of:

[0066] 1. Preparation and feeding of inducing substances: Prepare and feed according to the method of Chinese invention patent: 03113566.8 invented by the inventor. Use an aqueous solution of a mixture of hydrochloric acid, sodium carbonate, and potassium dihydrogen phosphate to control the concentration to 3-5%, and the pH to be about 6, and dissolve it in distilled water to obtain the inducer. Then put it into the cleaned blood-sucking leech living body, let the blood-sucking leech fully absorb satiety, just can obtain the blood-sucking leech living body after absorbing the inducing substance, and set aside.

[0067] 2. To induce vomiting:

[0068] (1) Prepare the emetic first: mix 2 kg of 0.5% zinc sulfate solution with 4 kg of 5% ethanol thoroughly to obtain the desired emetic and set aside.

[0069] (2) Emetic: Take 30 kg of the blood-sucking leech living body after absorbing the satie...

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Abstract

The invention discloses a large-scale production method for natural hirudin. The method comprises the following steps: placing a living hematophagous leech body which has thoroughly absorbed an induction liquid into a container and adding a certain amount of an emetic to allow the living hematophagous leech body to vomit saliva in its body, wherein the saliva is a crude natural hirudin product liquid, and the emetic is a zinc sulfate solution or a mixed liquor of zinc sulfate and ethanol; then taking out the living hematophagous leech body, flushing the living hematophagous leech body with clean water and continuing breeding the living hematophagous leech body for subsequent extraction; collecting the crude natural hirudin product liquid in the container; and adding a proper amount of a filler, carrying out mixing and then carrying out drying so as to obtain a finished powder product which is the natural hirudin product. According to the invention, a Markwardt thrombin direct titration method can be used to detect the content of active components in the natural hirudin product so as to guarantee stable quality and the curative effect of the product. The method has the characteristics of capacity of realizing large-scale production, simple process, a high recovery rate, low production cost and is capable of providing a safe and high-quality raw material for food, health food, medicines or cosmetics.

Description

technical field [0001] The invention relates to a method for extracting effective active ingredients, in particular to a production method for natural hirudin. Background technique [0002] Natural hirudin is a low-molecular-weight polypeptide composed of 65-66 amino acids. Its molecular weight is only 7000 Daltons. It is easily soluble in water and saline, and it is very stable at room temperature. Simply increasing the temperature (100°C water bath), Or pH change (1 ~ 13) did not affect its activity. Natural hirudin is the strongest natural antithrombin substance found in the world so far. It has special effects on human cardiovascular and cerebrovascular diseases, especially thrombotic diseases such as cerebral thrombosis. Its preventive effect is unmatched by any substance. At the same time, relevant research also shows that natural hirudin is a direct thrombin inhibitor, its anticoagulant effect is very specific, and its anticoagulant effect is stronger than that of he...

Claims

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Application Information

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IPC IPC(8): C07K14/815C07K1/14
CPCC07K14/815
Inventor 周维海
Owner 深圳金康道夫生物科技有限公司
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