Method for repeatedly extracting natural hirudin from live vampire leech

Abstract

The invention provides a method for repeatedly extracting natural hirudin from live vampire leech. The method comprises the steps of: firstly extracting crude natural hirudin from the live vampire leech, then carrying out separation and purification to obtain natural hirudin, wherein the extraction of the crude natural hirudin comprises the steps of: firstly preparing specific induction liquid and then pouring into intestinal canal to obtain specific induction liquid-containing intestinal canal; then feeding the specific inducing liquid-containing intestinal canal to the vampire leech, and extracting the spit of the vampire leech or grinding the vampire leech into slurry, thereby obtaining the crude natural hirudin; and the crude natural hirudin is separated and purified to obtain natural hirudin powder according to a conventional biochemical separation method. The method is simple in process, and high in extraction rate, the death rate of the vampire leeches can be obviously reduced after the crude natural hirudin is extracted, and biological resource utilization rate can be effectively improved, so that the production cost can be obviously lowered; therefore, the method is a convenient approach for extracting the main effective active substance (such as natural hirudin) from the leech body.

Description

technical field [0001] The invention relates to a production method for extracting effective active ingredients from animal bodies, in particular to a method for producing natural hirudin by using blood-sucking leeches as raw materials. Background technique [0002] Leeches, commonly known as leeches, are the general term for leeches. It is a traditional precious Chinese medicinal material in my country. It has the functions of dispelling blood and promoting menstrual flow, eliminating accumulation and stasis, reducing swelling and detoxification. It is one of the 35 important traditional Chinese medicinal materials for promoting blood circulation and removing blood stasis in my country. [0003] According to their feeding habits, leeches can be divided into two categories: non-blood-sucking leeches and blood-sucking leeches: [0004] Non-blood-sucking leech, it mainly feeds on the juice of snails (such as snails, apple snails, etc.), mussels and other molluscs, and does no...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: after the full-fed Hirudo phenanthae is artificially squeezed to make it secrete saliva, the oral cavity of Hirudo phenanthae is seriously damaged, which makes it easy to be infected with diseases or cause a large number of deaths due to its weakness and difficulty in recovery. At the same time, the time interval between the two extractions is too short, and Hirudo phenanthae cannot get nutritional supplements, and the body will not be fully recovered before entering the next extraction. In this way, on the one hand, the amount of crude hirudin extracted is small The content of hirudin is low, and more importantly, on the other hand, it will cause the death of Hirudo phenanthae to increase significantly, which will directly lead to high production costs

The disadvantage of this method is that the stimulating liquid contains neutral protease and papain, and these enzymes can destroy the chemical structure of hirudin, thereby reducing its activity and affecting the curative effect of hirudin.

The disadvantage of this method is: the use of chemical substances as the induction solution

The disadvantage of this method is that when a live leech is directly stimulated by a weak direct current, the leech will immediately shrink into a hard mass (because the leech is an invertebrate), and it is difficult to extract the hirudin

[0016] It can be seen from the above patents that chemical substances are used as the induction liquid to induce living blood-sucking leeches, and their safety has been questioned

At the same time, if the leech salivary gland secretion is taken out by artificial extrusion, that is, the natural hirudin crude product, the disadvantage is that there is no anti-infection drug in the induction solution, which will damage the oral cavity of the leech after extrusion, and directly cause the leech to be easily infected. Or frailty, decline in physical fitness, resulting in increased mortality, significantly increased production costs, and insignificant economic benefits