Oil-control moisturizing composition containing pleione bulbocodioides extract and application of oil-control moisturizing composition

Abstract

The invention relates to an oil-control moisturizing composition containing pleione bulbocodioides extract and application of the oil-control moisturizing composition. The composition is prepared from, by mass, 3-8 parts of pleione bulbocodioides extract, 1-5 parts of panax japonicus extract, 1-3 parts of sculellaria barbata extract and 1-2 parts of jasminum grandiflorum extract. The composition prepared by the invention is simple in components, all the components are natural plant sources, the safety is high, all the components have a mutual synergistic effect, and the oil control and moisturizing effects are remarkable.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine and relates to an oil-controlling and moisturizing composition containing a Herba Allii Alba extract and an application thereof. Background Art

[0002] Sebaceous glands, a key component of the skin, are responsible for the production of sebum. Excessive sebum production can lead to enlarged pores, oily and shiny skin, and even cause a range of issues such as breakouts and acne. Therefore, regulating sebaceous gland secretion and maintaining a balanced oil production are crucial for maintaining healthy skin.

[0003] The oil-control active ingredients in many commercially available products are mostly chemical ingredients or single plant-derived ingredients. These active ingredients either have unsafe safety or poor efficacy and cannot achieve a good oil-control effect. The prior art has developed compositions with oil-control and moisturizing effects. For example, CN 118490595 B provides a composition with oil-control and moisturizing effects, which includes capryloylglycine and Centella asiatica extract, a fermentation product of Candida albicans / glucose / rapeseed oleic acid methyl ester, hydroxy-madecassoside and glucose. The composition contains chemical ingredients and its safety cannot be fully guaranteed; while CN118045026A provides an anti-dandruff, oil-control and moisturizing composition, its preparation method and application. Although the composition is entirely composed of plant-derived active ingredients, there are 8 Chinese herbal medicines in the composition. Too many components may cause mutual interference between the components, increase the complexity of the formula and production costs, and increase the risk of side effects or allergic reactions.

[0004] Therefore, it is necessary to provide a composition with simple ingredients, strong safety, and both oil-controlling and moisturizing effects. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the present invention aims to provide an oil-control and moisturizing composition containing an extract of Orchidaceae and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides an oil-control and moisturizing composition containing a Herba Lycoris Radiatae extract, wherein the composition comprises, by mass, 3-8 parts of a Herba Lycoris Radiatae extract, 1-5 parts of a Panax Notoginseng extract, 1-3 parts of a Scutellaria barbata extract, and 1-2 parts of a Jasmine flower extract.

[0008] The mass proportions of the Orchidaceae extract can be selected as 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.; the mass proportions of the Panax notoginseng extract can be selected as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.; the mass proportions of the Scutellaria barbata extract can be selected as 1 part, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, 2.2 parts, 2.5 parts, 2.8 parts, 3 parts, etc.; the mass proportions of the Jasmine flower can be selected as 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc.; other specific values ​​within the above numerical range can be selected, so they will not be described one by one here.

[0009] Pleione bulbocodioides (Franch.) Rolfe is a dried pseudobulb from the Orchidaceae family. Panax japonicus is a dried rhizome from the Araliaceae family. Scutellaria barbata is the dried herb from the Lamiaceae family. Jasminum grandiflorum is the dried flower from the Oleaceae family.

[0010] The composition prepared by the present invention has simple ingredients, all of which are derived from natural plants, and is highly safe. The ingredients work synergistically with each other, resulting in significant oil-control and moisturizing effects. Furthermore, the extract prepared by the specific method exhibits even better oil-control and moisturizing effects.

[0011] Preferably, the Herba Lycoris radiata extract is prepared by a method comprising the following steps:

[0012] (1) mixing the orchid with water and performing ultrahigh pressure pretreatment to obtain a first mixture;

[0013] (2) extracting the first mixture using subcritical water, and filtering to obtain an extract;

[0014] (3) filtering the extract through a nanofiltration membrane to obtain a filtrate of the orchid;

[0015] (4) Mix the filtrate of the Herba Lycoris Radiatae with a decolorizing agent, filter the filtrate, and dry the filtrate to obtain the product.

[0016] Preferably, the ratio of the garlic orchid to water in (1) is 1 g: (10-20) mL, wherein the specific point values ​​in (10-20) can be selected from 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0017] Preferably, the ultrahigh pressure pretreatment is carried out at a temperature of 20-40° C., for 10-15 minutes, and at a pressure of 300-400 MPa.

[0018] The temperature can be selected as 20℃, 22℃, 25℃, 28℃, 30℃, 32℃, 35℃, 38℃, 40℃, etc. The time can be selected as 10min, 11min, 12min, 13min, 14min, 15min, etc. The pressure can be selected as 300MPa, 320MPa, 340MPa, 360MPa, 380MPa, 400MPa, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be listed here.

[0019] Preferably, the subcritical extraction pressure is 1-2 MPa, the temperature is 110-160° C., and the time is 20-40 min.

[0020] The pressure can be selected as 1MPa, 1.2MPa, 1.5MPa, 1.8MPa, 2MPa, etc. The temperature can be selected as 110℃, 115℃, 120℃, 125℃, 130℃, 135℃, 140℃, 145℃, 150℃, 155℃, 160℃, etc. The time can be selected as 20min, 22min, 25min, 28min, 30min, 32min, 35min, 38min, 40min, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be listed here.

[0021] Preferably, the subcritical extraction is performed 2-3 times.

[0022] Preferably, the pore size of the nanofiltration membrane is 1-10kDa, for example, 1kDa, 2kDa, 3kDa, 4kDa, 5kDa, 6kDa, 7kDa, 8kDa, 9kDa, 10kDa, etc. Other specific point values ​​within the above numerical range can be selected and will not be described here one by one.

[0023] Preferably, the mass ratio of the filtrate of the orchid to the decolorizer is 100: (0.1-1), and the specific point values ​​in (0.1-1) can be selected from 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0024] Preferably, the decolorizing agent is activated carbon and zeolite molecular sieve in a mass ratio of (1-3): 1. The specific values ​​of (1-3) can be selected from 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0025] Preferably, the pore size of the zeolite molecular sieve is For example Other specific point values ​​within the above numerical range can be selected and will not be described here one by one.

[0026] Preferably, the mixing temperature with the decolorizing agent is 40-70° C. and the mixing time is 30-50 min.

[0027] The temperature can be selected as 40℃, 45℃, 50℃, 55℃, 60℃, 65℃, 70℃, etc., and the time can be selected as 30min, 35min, 40min, 45min, 50min, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be listed here.

[0028] Preferably, the Scutellaria barbata extract is prepared by a method comprising the following steps:

[0029] (1) mixing Scutellaria barbata with an organic solvent, performing reflux extraction, and collecting the filter residue;

[0030] (2) mixing the filter residue with an ethanol aqueous solution, heating under reflux for extraction, and filtering to obtain an extract;

[0031] (3) The extract is enriched using a fine separation resin, and eluted with water and ethanol aqueous solution in sequence, and the ethanol aqueous solution eluate is collected, filtered, and dried to obtain the product.

[0032] Preferably, the organic solvent is petroleum ether and dichloromethane in a mass ratio of (2-4):1.

[0033] The specific point values ​​in (2-4) can all be selected from 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, and 4, so I will not go into details here.

[0034] Preferably, the ratio of the Scutellaria barbata to the organic solvent is 1 g: (10-20) mL.

[0035] The specific point values ​​in (10-20) can be selected as 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be listed here one by one.

[0036] Preferably, the reflux extraction time in step (1) is 2-3h, for example, 2h, 2.1h, 2.2h, 2.3h, 2.4h, 2.5h, 2.6h, 2.7h, 2.8h, 2.9h, 3h, etc. Other specific point values ​​within the above numerical range can be selected and will not be repeated here.

[0037] Preferably, the mass percentage of ethanol in the ethanol aqueous solution in step (2) is 20-40%, for example, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0038] Preferably, the material-liquid ratio of the filter residue to the ethanol aqueous solution in step (2) is 1 g:(10-15) mL, and the specific point values ​​in (10-15) can be selected from 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0039] Preferably, the time for the heating reflux extraction in step (2) is 2-3h, for example, 2h, 2.1h, 2.2h, 2.3h, 2.4h, 2.5h, 2.6h, 2.7h, 2.8h, 2.9h, 3h, etc. Other specific point values ​​within the above numerical range can be selected and will not be repeated here.

[0040] Preferably, the fine separation resin in step (3) comprises SP20SS or HP20SS.

[0041] Preferably, the mass percentage of ethanol in the ethanol aqueous solution in step (3) is 20-30%, for example, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0042] Preferably, the elution volume of water in step (3) is 4-6 bv, such as 4 bv, 5 bv, 6 bv, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0043] Preferably, the elution volume of the ethanol aqueous solution in step (3) is 4-6 bv, such as 4 bv, 5 bv, 6 bv, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0044] Preferably, the Panax japonicus extract is prepared by a method comprising the following steps:

[0045] (1) mixing bamboo nodes with ethanol and aqueous solution, performing reflux extraction, and filtering to obtain an extract;

[0046] (2) Filtering the extract and concentrating it to obtain a crude extract, mixing the crude extract with water, and applying it to a macroporous resin;

[0047] (3) using water and ethanol aqueous solution to elute in sequence, collecting the ethanol aqueous solution eluate, filtering and drying to obtain the product.

[0048] Preferably, the solid-liquid ratio of the bamboo joint to the ethanol aqueous solution is 1g:(8-15)mL, and the specific point values ​​in (8-15) can be selected from 8, 9, 10, 11, 12, 13, 14, 15, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0049] Preferably, the mass percentage of ethanol in the ethanol aqueous solution in step (1) is 30-50%, for example, 30%, 32%, 34%, 36%, 38%, 40%, 42%, 44%, 46%, 48%, 50%, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0050] Preferably, the reflux extraction time is 1.5-3h, for example, 1.5h, 1.8h, 2h, 2.2h, 2.5h, 2.8h, 3h, etc. Other specific point values ​​within the above numerical range can be selected and will not be repeated here.

[0051] Preferably, the mass ratio of the crude extract to water is 1:(8-10), and the specific point values ​​in (8-10) can be selected from 8, 8.2, 8.4, 8.6, 8.8, 9, 9.2, 9.4, 9.6, 9.8, 10, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0052] Preferably, the elution volume of water in step (3) is 4-7 bv, such as 4 bv, 5 bv, 6 bv, 7 bv, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0053] Preferably, the elution volume of the ethanol aqueous solution in step (3) is 4-6 bv, such as 4 bv, 5 bv, 6 bv, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0054] Preferably, the mass percentage of ethanol in the ethanol aqueous solution in step (3) is 30-40%, for example, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0055] Preferably, the frangipani flower extract is prepared by a method comprising the following steps:

[0056] (1) mixing frangipani flowers with ethanol aqueous solution, heating and refluxing for extraction, and filtering to obtain an extract;

[0057] (2) Decolorize the extract with activated carbon, filter, concentrate and dry to obtain the product.

[0058] Preferably, the mass percentage of ethanol in the ethanol aqueous solution in step (1) is 20-40%, for example, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, 40%, etc. Other specific values ​​within the above numerical range can be selected and will not be repeated here.

[0059] Preferably, the material-liquid ratio of frangipani flowers to ethanol aqueous solution is 1g:(10-15)mL, and the specific point values ​​in (10-15) can be selected from 10, 11, 12, 13, 14, 15, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0060] Preferably, the reflux extraction time is 2-3h, for example, 2h, 2.2h, 2.4h, 2.6h, 2.8h, 3h, etc. Other specific point values ​​within the above numerical range can be selected and will not be repeated here.

[0061] Preferably, the mass ratio of the extract to the activated carbon is (80-100):1, and the specific point values ​​in (80-100) can be selected from 80, 82, 85, 88, 90, 92, 95, 98, 100, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here.

[0062] In a second aspect, the present invention provides a use of the oil-control and moisturizing composition containing the Herba Allii subtilis extract according to the first aspect in the preparation of an AQP-3 gene activator or a sebaceous gland cell oil secretion inhibitor.

[0063] According to the research results of the present invention, the composition prepared by the present invention can activate the expression of the AQP-3 gene and inhibit the secretion of oil in sebaceous gland cells at the cellular level (in vitro level). It is used to explore the expression process of the AQP-3 gene and the secretion of oil in sebaceous gland cells, that is, it is an application in the preparation of an AQP-3 gene activator or a sebaceous gland cell oil secretion inhibitor.

[0064] In a third aspect, the present invention provides a use of the oil-control and moisturizing composition containing the Herba Lycoris Radiatae extract according to the first aspect in preparing a product with oil-control or moisturizing efficacy.

[0065] Preferably, the product comprises a cosmetic or skin care product.

[0066] Compared with the prior art, the present invention has the following beneficial effects:

[0067] 1. The present invention adopts specific extraction processes to effectively extract the active ingredients from Orchidaceae, Panax notoginseng, Scutellaria barbata and Jasmine flower, and successfully obtains a composition with oil-control and moisturizing effects.

[0068] 2. The composition obtained by the specific preparation method of the present invention can significantly inhibit the secretion of oil by sebaceous gland cells and promote the relative expression of aquaporin-3 (AQP-3) mRNA.

[0069] 3. The composition obtained by the preparation method of the present invention has simple ingredients and is all derived from natural plants, with high safety. The ingredients have a synergistic effect and have significant oil-control and moisturizing effects. The extract prepared by the specific method has better oil-control and moisturizing effects. DETAILED DESCRIPTION

[0070] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0071] The sources of the functional ingredients contained in the products involved in the following examples and comparative examples are as follows (only the functional ingredients are reflected, and the necessary auxiliary ingredients contained in other commercially available raw materials are not repeated):

[0072] The garlic orchid was purchased from Zhaotong Yunsheng Agricultural Science and Technology Development Co., Ltd.

[0073] Panax notoginseng, frangipani, and Scutellaria barbata were purchased from Yunnan Lvsheng Pharmaceutical Co., Ltd.

[0074] Petroleum ether was purchased from Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. and was of analytical grade with a boiling point range of 30-60°C.

[0075] Preparation Example 1-1

[0076] This preparation example provides a method for preparing a Herba Lycopodii extract, the preparation method comprising:

[0077] (1) The medicinal material of the Herba Lycoris radiata was crushed and mixed with water, and then placed in an ultrahigh pressure device for ultrahigh pressure pretreatment. The treatment pressure was 350 MPa, the pressure holding time was 12 min, the temperature was 25 ° C, and the material-liquid ratio of the Herba Lycoris radiata to water was 1 g: 15 mL to obtain a Herba Lycoris radiata mixed solution;

[0078] (2) The mixed solution of the orchid was transferred to a subcritical extraction kettle, the extraction temperature was 140 ° C, the extraction pressure was 1.5 MPa, the extraction time was 30 min, the extraction times were 3 times, and the extract was obtained after filtration;

[0079] (3) treating the extract obtained in step (2) with a 5 kDa nanofiltration membrane to obtain a membrane filtrate;

[0080] (4) 0.5 wt% of decolorizing agent is added to the membrane filtrate, wherein the decolorizing agent is activated carbon and zeolite molecular sieve (zeolite molecular sieve effective pore size is 2:1) in a mass ratio. ), stirred at 50°C for 40 min, filtered, concentrated and then dried to obtain the product.

[0081] Preparation Example 1-2

[0082] This preparation example provides a method for preparing a Herba Lycopodii extract, the preparation method comprising:

[0083] (1) The medicinal material of the Herba Lycoris radiata was crushed and mixed with water, and then placed in an ultrahigh pressure device for ultrahigh pressure pretreatment at a pressure of 300 MPa, a holding time of 15 min, a temperature of 40° C., and a material-liquid ratio of the Herba Lycoris radiata to water of 1 g:20 mL to obtain a Herba Lycoris radiata mixed solution;

[0084] (2) The mixed solution of the Orchidaceae was transferred to a subcritical extraction kettle, the extraction temperature was 120 ° C, the extraction pressure was 1 MPa, the extraction time was 40 min, the number of extractions was 3, and the extract was obtained after merging and filtering.

[0085] (3) treating the extract obtained in step (2) with a 1 kDa nanofiltration membrane to obtain a membrane filtrate;

[0086] (4) 0.9 wt% of decolorizing agent is added to the membrane filtrate, wherein the decolorizing agent is activated carbon and zeolite molecular sieve (zeolite molecular sieve effective pore size is 1:1) in a mass ratio. ), stirred at 40°C for 50 min, filtered, concentrated and then dried to obtain the product.

[0087] Preparation Examples 1-3

[0088] This preparation example provides a method for preparing a Herba Lycopodii extract, the preparation method comprising:

[0089] (1) The medicinal material of the Herba Lycoris radiata was crushed and mixed with water, and then placed in an ultrahigh pressure device for ultrahigh pressure pretreatment, with a treatment pressure of 400 MPa, a holding time of 10 min, a temperature of 30° C., and a material-liquid ratio of the Herba Lycoris radiata to water of 1 g:10 mL to obtain a Herba Lycoris radiata mixed solution;

[0090] (2) The mixed solution of the orchid was transferred to a subcritical extraction kettle, the extraction temperature was 160 ° C, the extraction pressure was 2 MPa, the extraction time was 20 min, the extraction times were 3 times, and the extract was obtained after filtration;

[0091] (3) treating the extract obtained in step (2) with a 10 kDa nanofiltration membrane to obtain a membrane filtrate;

[0092] (4) 0.1 wt% of decolorant is added to the membrane filtrate, wherein the decolorant is activated carbon and zeolite molecular sieve (zeolite molecular sieve effective pore size is 3:1) in a mass ratio. ), stirred at 70°C for 30 min, filtered, concentrated and then dried to obtain the product.

[0093] Preparation Examples 1-4

[0094] This preparation example provides a preparation method of a Herba Lycoris radiata extract, which differs from Preparation Example 1-1 only in that step (1) is "crushing the Herba Lycoris radiata medicinal material and mixing it with water, with the material-liquid ratio of the Herba Lycoris radiata medicinal material to water being 1 g:15 mL to obtain a Herba Lycoris radiata mixed solution", and other operations remain unchanged.

[0095] Preparation Examples 1-5

[0096] This preparation example provides a preparation method of a Herba Lycoris Radiata extract, which differs from Preparation Example 1-1 only in that step (1) is "crushing the Herba Lycoris Radiata medicinal materials and mixing them with water, with a solid-liquid ratio of Herba Lycoris Radiata medicinal materials to water being 1 g:15 mL, ultrasonicating three times for 1 h each time at an ultrasonic power of 600 W to obtain a Herba Lycoris Radiata mixed solution", and other operations remain unchanged.

[0097] Preparation Examples 1-6

[0098] This preparation example provides a preparation method of a Herba Lycoris radiata extract, which differs from Preparation Example 1-1 only in that step (2) is "extracting the Herba Lycoris radiata mixed solution by heating and refluxing for 30 minutes, extracting three times, and combining the extracts", and other operations remain unchanged.

[0099] Preparation Examples 1-7

[0100] This preparation example provides a preparation method of a Herba Lycopersicum L. extract, which differs from Preparation Example 1-1 only in that step (4) is "adding 0.5 wt % of a decolorizing agent to the membrane filtrate, wherein the decolorizing agent is activated carbon, stirring at 50° C. for 40 min, filtering, concentrating, and then drying to obtain the extract", and other operations remain unchanged.

[0101] Preparation Examples 1-8

[0102] This preparation example provides a preparation method of a garlic orchid extract, which differs from Preparation Example 1-1 only in that step (4) is "adding 0.5 wt% of a decolorizing agent to the membrane filtrate, wherein the decolorizing agent is a zeolite molecular sieve (the effective pore size of the zeolite molecular sieve is ), stirred at 50°C for 40 min, filtered, concentrated and then dried to obtain ". Other operations remain unchanged.

[0103] Preparation Example 2-1

[0104] This preparation example provides a method for preparing a Panax japonicus extract, the preparation method comprising:

[0105] (1) Grinding the Panax japonicus and mixing it with a 40% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, heating and refluxing for 2 h to obtain an extract;

[0106] (2) Filter the extract and concentrate to obtain a crude extract, mix the crude extract with 8 times the mass of water, and apply it to AB-8 macroporous resin;

[0107] (3) Use 5 bv of water and 5 bv of 30% ethanol aqueous solution to elute in sequence, collect the 30% ethanol aqueous solution eluate, filter it, concentrate and dry it to obtain the product.

[0108] Preparation Example 2-2

[0109] This preparation example provides a method for preparing a Panax japonicus extract, the preparation method comprising:

[0110] (1) Grinding the Panax japonicus and mixing it with a 30% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:8 mL, heating and refluxing for 3 h to obtain an extract;

[0111] (2) Filter the extract and concentrate to obtain a crude extract, mix the crude extract with 10 times the mass of water, and apply it to AB-8 macroporous resin;

[0112] (3) eluting with 5 bv of water and 5 bv of 40% by mass ethanol aqueous solution in sequence, collecting the 40% ethanol aqueous solution eluate, filtering it, concentrating it and drying it to obtain the product.

[0113] Preparation Example 2-3

[0114] This preparation example provides a method for preparing a Panax japonicus extract, the preparation method comprising:

[0115] (1) Grinding the Panax japonicus and mixing it with a 50% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:15 mL, heating and refluxing for 1.5 h to obtain an extract;

[0116] (2) The extract was filtered and concentrated to obtain a crude extract, which was mixed with 9 times the mass of water and loaded onto D-101 macroporous resin;

[0117] (3) Use 5 bv of water and 5 bv of 35% by mass ethanol aqueous solution to elute in sequence, collect the 35% ethanol aqueous solution eluate, filter it, concentrate and dry it to obtain the product.

[0118] Preparation Example 3-1

[0119] This preparation example provides a preparation method of Scutellaria barbata extract, which comprises:

[0120] (1) Scutellaria barbata was crushed and mixed with an organic solvent, and then heated under reflux for extraction. The ratio of Scutellaria barbata to the organic solvent was 1 g:15 mL, and the organic solvent was petroleum ether and dichloromethane in a mass ratio of 3:1. The extraction time was 2.5 h. After the extraction was completed, the filter was filtered, the filter residue was collected, and dried;

[0121] (2) The dried filter residue was mixed with a 30% by weight ethanol aqueous solution at a solid-liquid ratio of 1 g:12 mL, and the mixture was heated under reflux for 2.5 h. After the extraction was completed, the extract was filtered and collected;

[0122] (3) The extract was concentrated and then enriched with a fine separation resin (Mitsubishi SP20SS), first eluted with water for 5 column volumes, and then eluted with 25% by weight ethanol aqueous solution for 5 column volumes, the ethanol eluate was collected, filtered and dried.

[0123] Preparation Example 3-2

[0124] This preparation example provides a preparation method of Scutellaria barbata extract, which comprises:

[0125] (1) Scutellaria barbata was crushed and mixed with an organic solvent, and then heated under reflux for extraction. The ratio of Scutellaria barbata to the organic solvent was 1 g:10 mL, and the organic solvent was petroleum ether and dichloromethane in a mass ratio of 2:1. The extraction time was 2 h. After the extraction was completed, the filter was filtered, the filter residue was collected, and dried;

[0126] (2) The dried filter residue was mixed with a 20% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:15 mL, and the mixture was heated under reflux for 2 h. After the extraction was completed, the extract was filtered and collected;

[0127] (3) The extract was concentrated and then enriched with a fine separation resin (Mitsubishi HP20SS), first eluted with water for 5 column volumes, and then eluted with a 20% by weight ethanol aqueous solution for 5 column volumes, the ethanol eluate was collected, filtered and dried.

[0128] Preparation Example 3-3

[0129] This preparation example provides a preparation method of Scutellaria barbata extract, which comprises:

[0130] (1) Scutellaria barbata was crushed and mixed with an organic solvent, and then heated under reflux for extraction. The ratio of Scutellaria barbata to the organic solvent was 1 g: 20 mL, and the organic solvent was petroleum ether and dichloromethane in a mass ratio of 4:1. The extraction time was 3 h. After the extraction was completed, the filter was filtered, the filter residue was collected, and dried;

[0131] (2) The dried filter residue was mixed with a 40% by weight ethanol aqueous solution at a solid-liquid ratio of 1 g:10 mL, and the mixture was heated under reflux for 3 h. After the extraction was completed, the extract was filtered and collected;

[0132] (3) The extract was concentrated and then enriched with a fine separation resin (Mitsubishi SP20SS), first eluted with water for 5 column volumes, and then eluted with 30% by weight ethanol aqueous solution for 5 column volumes, the ethanol eluate was collected, filtered and dried.

[0133] Preparation Example 3-4

[0134] This preparation example provides a preparation method of Scutellaria barbata extract, which differs from Preparation Example 3-1 only in that step (1) is "crushing the Scutellaria barbata and mixing it with an organic solvent, heating and refluxing for extraction, the ratio of Scutellaria barbata to the organic solvent is 1 g:15 mL, the organic solvent is petroleum ether, the extraction time is 2.5 h, filtering after the extraction is completed, collecting the filter residue, and drying", and other operations remain unchanged.

[0135] Preparation Example 3-5

[0136] This preparation example provides a preparation method of Scutellaria barbata extract, which differs from Preparation Example 3-1 only in that step (1) is "crushing the Scutellaria barbata and mixing it with an organic solvent, heating and refluxing for extraction, the ratio of Scutellaria barbata to the organic solvent is 1 g:15 mL, the organic solvent is dichloromethane, the extraction time is 2.5 h, filtering after the extraction is completed, collecting the filter residue, and drying", and other operations remain unchanged.

[0137] Preparation Examples 3-6

[0138] This preparation example provides a method for preparing a Scutellaria barbata extract, which differs from Preparation Example 3-1 only in that step (3) is "concentrating the extract and then enriching it with AB-8 macroporous resin, eluting it with water for 5 column volumes, then eluting it with 25% by weight ethanol aqueous solution for 5 column volumes, collecting the ethanol eluate, filtering it, and drying it to obtain the extract", and the other operations remain unchanged.

[0139] Preparation Example 3-7

[0140] This preparation example provides a method for preparing a Scutellaria barbata extract, which differs from Preparation Example 3-1 only in that step (3) is "concentrating the extract and enriching it with a fine separation resin (Mitsubishi SP20SS), eluting it with water for 5 column volumes, and then eluting it with a 10% by weight ethanol aqueous solution for 5 column volumes, collecting the ethanol eluate, filtering it, and drying it to obtain the extract." The other operations remain unchanged.

[0141] Preparation Example 3-8

[0142] This preparation example provides a method for preparing a Scutellaria barbata extract, which differs from Preparation Example 3-1 only in that step (3) is "concentrating the extract and enriching it with a fine separation resin (Mitsubishi SP20SS), eluting it with water for 5 column volumes, and then eluting it with a 40% by weight ethanol aqueous solution for 5 column volumes, collecting the ethanol eluate, filtering it, and drying it to obtain the extract." The other operations remain unchanged.

[0143] Preparation Example 3-9

[0144] This preparation example provides a preparation method of Scutellaria barbata extract, which comprises:

[0145] (1) Scutellaria barbata was crushed and mixed with a 30% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:12 mL, and the mixture was heated under reflux for 2.5 h. After the extraction was completed, the extract was filtered and collected;

[0146] (2) The extract was concentrated and then enriched with a fine separation resin (Mitsubishi SP20SS), first eluted with water for 5 column volumes, and then eluted with 25% by weight ethanol aqueous solution for 5 column volumes, the ethanol eluate was collected, filtered and dried to obtain the product.

[0147] Preparation Example 4-1

[0148] This preparation example provides a preparation method of a frangipani flower extract, which comprises:

[0149] (1) Mixing frangipani flowers with 30% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:12 mL, heating and refluxing for 2.5 h, and filtering to obtain an extract;

[0150] (2) The extract is decolorized with activated carbon, with the mass ratio of the extract to the activated carbon being 90:1, filtered, concentrated and dried to obtain the product.

[0151] Preparation Example 4-2

[0152] This preparation example provides a preparation method of a frangipani flower extract, which comprises:

[0153] (1) Mixing frangipani flowers with 20% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:15 mL, heating and refluxing for 2 h, and filtering to obtain an extract;

[0154] (2) The extract is decolorized with activated carbon, with the mass ratio of the extract to the activated carbon being 80:1, filtered, concentrated and dried to obtain the product.

[0155] Preparation Example 4-3

[0156] This preparation example provides a preparation method of a frangipani flower extract, which comprises:

[0157] (1) Mixing frangipani flowers with 40% by weight ethanol aqueous solution at a material-liquid ratio of 1 g:10 mL, heating and refluxing for 3 h, and filtering to obtain an extract;

[0158] (2) Decolorize the extract with activated carbon, with the mass ratio of the extract to the activated carbon being 100:1, filter, concentrate and dry to obtain the product.

[0159] Example 1

[0160] This embodiment provides an oil-controlling and moisturizing composition, which includes, by mass, 5 parts of the Orchidaceae extract prepared in Preparation Example 1-1, 3 parts of the Panax notoginseng extract prepared in Preparation Example 2-1, 2 parts of the Scutellaria barbata extract prepared in Preparation Example 3-1, and 1.5 parts of the Jasmine flower extract prepared in Preparation Example 4-1.

[0161] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0162] Example 2

[0163] This embodiment provides an oil-controlling and moisturizing composition, which includes, by mass, 3 parts of the Orchidaceae extract prepared in Preparation Example 1-2, 5 parts of the Panax notoginseng extract prepared in Preparation Example 2-2, 1 part of the Scutellaria barbata extract prepared in Preparation Example 3-2, and 2 parts of the Jasmine flower extract prepared in Preparation Example 4-2.

[0164] The preparation method is as in Example 1.

[0165] Example 3

[0166] This embodiment provides an oil-controlling and moisturizing composition, which includes, by mass, 8 parts of the Orchidaceae extract prepared in Preparation Example 1-3, 1 part of the Panax notoginseng extract prepared in Preparation Example 2-3, 3 parts of the Scutellaria barbata extract prepared in Preparation Example 3-3, and 1 part of the Jasmine flower extract prepared in Preparation Example 4-3.

[0167] The preparation method is as in Example 1.

[0168] Examples 4-8

[0169] This example provides five oil-control and moisturizing compositions, which differ from Example 1 only in that the Orchidaceae extract prepared in Preparation Example 1-1 is replaced in equal amounts by the Orchidaceae extract prepared in Preparation Example 1-4, the Orchidaceae extract prepared in Preparation Example 1-5, the Orchidaceae extract prepared in Preparation Example 1-6, the Orchidaceae extract prepared in Preparation Example 1-7, and the Orchidaceae extract prepared in Preparation Example 1-8, and the other ingredients and contents remain unchanged.

[0170] The preparation method is as in Example 1.

[0171] Examples 9-14

[0172] This example provides 6 oil-controlling and moisturizing compositions, which differ from Example 1 only in that the Scutellaria baicalensis extract prepared in Preparation Example 3-1 is replaced in equal amounts by the Scutellaria baicalensis extract prepared in Preparation Example 3-4, the Scutellaria baicalensis extract prepared in Preparation Example 3-5, the Scutellaria baicalensis extract prepared in Preparation Example 3-6, the Scutellaria baicalensis extract prepared in Preparation Example 3-7, the Scutellaria baicalensis extract prepared in Preparation Example 3-8, and the Scutellaria baicalensis extract prepared in Preparation Example 3-9, and the other ingredients and contents remain unchanged.

[0173] The preparation method is as in Example 1.

[0174] Comparative Example 1

[0175] This comparative example provides an oil-control moisturizing composition, which differs from Example 1 only in that it does not contain the Orchidaceae extract prepared in Preparation Example 1-1, and its reduced mass is proportionally distributed to the mass of the Panax notoginseng extract prepared in Preparation Example 2-1, the Scutellaria barbata extract prepared in Preparation Example 3-1, and the Jasmine flower extract prepared in Preparation Example 4-1.

[0176] The preparation method is as in Example 1.

[0177] Comparative Example 2

[0178] This comparative example provides an oil-control moisturizing composition, which differs from Example 1 only in that it does not contain the Panax japonicus extract prepared in Preparation Example 2-1, and its reduced mass is proportionally distributed to the mass of the Orchidaceae extract prepared in Preparation Example 1-1, the Scutellaria barbata extract prepared in Preparation Example 3-1, and the Jasmine flower extract prepared in Preparation Example 4-1.

[0179] The preparation method is as in Example 1.

[0180] Comparative Example 3

[0181] This comparative example provides an oil-control moisturizing composition, which differs from Example 1 only in that it does not contain the Scutellaria barbata extract prepared in Preparation Example 3-1, and its reduced mass is proportionally distributed to the mass of the Orchidaceae extract prepared in Preparation Example 1-1, the Panax notoginseng extract prepared in Preparation Example 2-1, and the Jasmine flower extract prepared in Preparation Example 4-1.

[0182] The preparation method is as in Example 1.

[0183] Comparative Example 4

[0184] This comparative example provides an oil-control moisturizing composition, which differs from Example 1 only in that it does not contain the frangipani flower extract prepared in Preparation Example 4-1, and its reduced mass is proportionally distributed to the mass of the Orchidaceae extract prepared in Preparation Example 1-1, the Panax notoginseng extract prepared in Preparation Example 2-1, and the Scutellaria barbata extract prepared in Preparation Example 3-1.

[0185] The preparation method is as in Example 1.

[0186] Test Example 1

[0187] The SZ95 sebaceous gland cells used in this experiment were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences. 5α-dihydrotestosterone (DHT) was used as an induction condition for lipid droplets, and isotretinoin was used as a positive marker. The oil-control efficacy of the test samples was evaluated by measuring their inhibitory effect on sebum secretion.

[0188] Test method: press 1×10 5 Cells were seeded into 24-well plates at a seeding density of 100 cells / well and incubated overnight in an incubator (37°C, 5% CO2). When the cell plating rate in the 24-well plate reached 40% to 60%, the cells were divided into groups for drug administration: a blank group (cultured with culture medium only, without any treatment), a negative control group (treated with 10% DMSO solution), a positive control group (treated with 3 μg / mL isotretinoin), and a sample group (treated with 120 μg / mL dissolved in 10% DMSO). All groups, except the blank group, were induced with DHT. Three replicates were set up for each group and the cells were cultured in an incubator (37°C, 5% CO2) for 24 hours. The culture medium was discarded and the cells were rinsed with PBS. After fixation, cells were stained with Nile red for 15 minutes. After rinsing, fluorescence intensity was recorded using a fluorescence microscope, and Image Pro Plus software was used for quantitative analysis.

[0189]

[0190] The compositions obtained in Examples 1-14 and Comparative Examples 1-4 were evaluated for their cell oil control efficacy. The results are shown in Table 1:

[0191] Table 1

[0192]

[0193]

[0194] As can be seen from the data in Table 1, the composition prepared by the present invention has a strong oil-control effect, among which the Herba Lycoris Radiatae Extract, the Herba Panacis Japonicae Extract, the Herba Scutellariae Barbatae Extract, and the Herba Jasminoides Extract have a certain synergistic effect on the above-mentioned effects, and the preparation method of the extract will affect the efficacy of the composition. A specific extraction method can better promote the efficacy of the composition.

[0195] Test Example 2

[0196] Moisturizing activity test:

[0197] Test method:

[0198] Aquaporin-3 (AQP-3) plays an important role in maintaining skin moisture and skin function. The experiment used fluorescent quantitative PCR to determine the effect of samples on the expression of aquaporin-3. HaCaT cells were seeded in 24-well plates. The experiment set up a normal control group, a positive control group (CaCl2), and a sample administration group (120 μg / mL). The cells were incubated in an incubator (37°C, 5% CO2) for 12 hours. When the plating rate reached 40-60%, the cells were divided into groups and dosed. After the administration was completed, the cells were incubated in the incubator for 24 hours. After the incubation, RNA was determined using a total RNA extraction kit, and AQP3 gene expression was detected by fluorescent quantitative PCR.

[0199] The compositions obtained in Examples 1-14 and Comparative Examples 1-4 were evaluated for their cellular moisturizing efficacy. The results are shown in Table 2:

[0200] Table 2

[0201]

[0202]

[0203] It can be seen from the data in Table 2 that the composition prepared by the present invention has a strong moisturizing effect, among which the Herba Lycoris Radiatae extract, the Herba Panacis Scutellariae extract, the Herba Scutellariae Scutellariae extract, and the Herba Jasminoides extract have a certain synergistic effect on the above-mentioned effects, and the preparation method of the extract will affect the efficacy of the composition. A specific extraction method can better promote the efficacy of the composition.

[0204] The applicant declares that the present invention illustrates an oil-control and moisturizing composition containing a Herba Allii var. uniflora extract and its application through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.

[0205] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0206] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. An oil-controlling and moisturizing composition containing a Herba Lycoris Radiatae extract, characterized in that: The composition comprises, by mass, 3-8 parts of Orchidaceae extract, 1-5 parts of Panax notoginseng extract, 1-3 parts of Scutellaria barbata extract, and 1-2 parts of Jasmine frangipani extract.

2. The oil-controlling and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 1, wherein: The extract of Orchidaceae is prepared by a method comprising the following steps: (1) mixing the orchid with water and performing ultrahigh pressure pretreatment to obtain a first mixture; (2) extracting the first mixture using subcritical water, and filtering to obtain an extract; (3) filtering the extract through a nanofiltration membrane to obtain a filtrate of the orchid; (4) Mix the filtrate of the Herba Lycoris Radiatae with a decolorizing agent, filter the filtrate, and dry the filtrate to obtain the product.

3. The oil-controlling and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 2, wherein: The ultra-high pressure pretreatment is carried out at a temperature of 20-40° C., for a time of 10-15 minutes, and at a pressure of 300-400 MPa.

4. The oil-controlling and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 2, wherein: The decolorizing agent is activated carbon and zeolite molecular sieve in a mass ratio of (1-3):

1.

5. The oil-controlling and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 1, wherein: The Scutellaria barbata extract is prepared by a method comprising the following steps: (1) mixing Scutellaria barbata with an organic solvent, performing reflux extraction, and collecting the filter residue; (2) mixing the filter residue with an ethanol aqueous solution, heating under reflux for extraction, and filtering to obtain an extract; (3) The extract is enriched using a fine separation resin, and eluted with water and ethanol aqueous solution in sequence, and the ethanol aqueous solution eluate is collected, filtered, and dried to obtain the product.

6. The oil-controlling and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 5, characterized in that: The organic solvent is petroleum ether and dichloromethane in a mass ratio of (2-4):1; The mass percentage of ethanol in the ethanol aqueous solution in step (3) is 20-30%.

7. The oil-control and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 1, wherein: The Panax japonicus extract is prepared by a method comprising the following steps: (1) mixing bamboo nodes with ethanol and aqueous solution, performing reflux extraction, and filtering to obtain an extract; (2) Filtering the extract and concentrating it to obtain a crude extract, mixing the crude extract with water, and applying it to a macroporous resin; (3) using water and ethanol aqueous solution to elute in sequence, collecting the ethanol aqueous solution eluate, filtering and drying to obtain the product.

8. The oil-control and moisturizing composition containing the Herba Lycoris Radiatae extract according to claim 1, wherein: The frangipani flower extract is prepared by a method comprising the following steps: (1) mixing frangipani flowers with ethanol aqueous solution, performing reflux extraction, and filtering to obtain an extract; (2) Decolorize the extract with activated carbon, filter, concentrate and dry to obtain the product.

9. Use of the oil-control and moisturizing composition containing the Herba Lycoris Radiatae extract according to any one of claims 1 to 8 in the preparation of an AQP-3 gene activator or a sebaceous gland cell oil secretion inhibitor.

10. Use of the oil-control and moisturizing composition containing the Herba Lycoris Radiatae extract according to any one of claims 1 to 8 in preparing a product with oil-control or moisturizing efficacy.

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