Chromosome structure variation molecular marker related to tomato fruit color, specific primers and application thereof

A specific primer and chromosome technology, applied in the field of plant genetic engineering and molecular biology, can solve the problem of uneven accumulation of anthocyanins, and achieve the effect of accelerating the breeding process, reducing the investment of planting area and human and material resources, and improving the breeding efficiency.

Active Publication Date: 2019-09-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Two mutants, Aft (Anthocyanin fruit) and atv (Atroviolacium), can accumu...

Method used

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  • Chromosome structure variation molecular marker related to tomato fruit color, specific primers and application thereof
  • Chromosome structure variation molecular marker related to tomato fruit color, specific primers and application thereof
  • Chromosome structure variation molecular marker related to tomato fruit color, specific primers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Test material

[0035] The ginkgo tomato (hereinafter referred to as wf mutant) used in this experiment is a natural mutant, the fruit is yellow-white when immature, and red when ripe; Ailsa Craig (AC) is a normal tomato, green when immature, and red when ripe . The F1 generation was obtained by crossing AC as the female parent, the wf mutant was obtained by crossing the male parent, and the F2 was obtained by selfing F1.

[0036] Identification and marker development of chromosomal structural variation in wf mutants

[0037] According to the similar phenotype between the wf mutant and the luteescent2 mutant, we first performed PCR detection on the luteescent2 gene in the wf mutant, and found that no band could be amplified when the full length of the luteescent2 gene in the wf mutant was amplified by PCR , so we amplified the luteescent2 gene in segments, and found that there may be a mutation from the 5886th base to the 6156th base after the start codon of the lutee...

Embodiment 2

[0041] 5. On the basis of Example 1, we first designed primer SH1 with the sequence of about 1000 bp at both ends of the wf mutant inversion site 62527986 bases as a template, so that it can amplify to about 1000 bp band in the wf mutant ( For the specific sequence, refer to the sequence table (SEQ ID NO: 5), no band can be amplified in AC; the primer SH2 is designed with the sequence of about 1500 bp at both ends of the normal tomato 62527986 base as a template, so that it can amplify about 1500 bp in AC. A 1500bp band (see SEQ ID NO: 6 in the sequence listing for the specific sequence), but no band can be amplified in the wf mutant.

[0042] Using primers SH1 and SH2 to carry out molecular detection on wf mutant, Ailsa Craig (AC), F1 generation, F2 generation plants, the results showed that in AC, SH1 could not amplify any band, and SH2 could amplify a 1548bp band ; In the wf mutant, SH1 can be amplified to a 986bp band, SH2 can not be amplified to any band; in the F1 genera...

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Abstract

The invention belongs to the field of plant genetic engineering and molecular biology, particularly relates to an identification method for identifying whether the filial generation has white tomato fruits or not, specific primers used by the method and application of the specific primers and the method, and provides a chromosome structure variation (SV) molecular marker related to tomato fruit color. The molecular marker utilizes the 208kb inversion, at chromosome 10, of a white-fruit mutant, and the physical position of the inversion is located from the 62736140th base of chromosome 10 to the 62527986th base of chromosome 10. The two pairs specific primers can be used to identify whether the tomato filial generation has white fruits or not and whether the tomato filial generation is in a heterozygosis state or not. The method uses the molecular marker and the specific primers to identify whether the tomato filial generation has white fruits. By the molecular marker, the specific primers and the method, the white-fruit tomato materials can be fast selected, breeding cycle can be shortened, and breeding efficiency can be increased.

Description

technical field [0001] The invention belongs to the fields of plant genetic engineering and molecular biology, and specifically relates to a method for identifying whether a hybrid offspring is a white tomato fruit, a specific primer and an application thereof. Background technique [0002] Tomato is an important horticultural crop worldwide, and its fruit is bright and colorful. Different colors not only give tomato fruit a beautiful appearance, but also have high nutritional value. A variety of pigments in tomatoes such as carotenoids and anthocyanins have the functions of anti-oxidation, scavenging free radicals, delaying aging and preventing cancer. Therefore, tomato, as an important vegetable, has always been loved by people. With the improvement of our people's living standards, people's consumption demand for horticultural products has increased greatly, not only the demand for output, but also the demand for multiple samples. Therefore, cultivating tomatoes of dif...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张俊红李国斌叶志彪张余洋王家发艾国郑方燕
Owner HUAZHONG AGRI UNIV
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