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Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings

A technology for culture medium and virus-free seedlings, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of light leaf color, shrinkage, pollution, etc., achieve good genetic stability, reduce the proportion of browning, The effect of high reproduction coefficient

Inactive Publication Date: 2011-01-19
北京金六环农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fruit is conical, the seed is yellow and slightly green, slightly concave on the surface of the fruit, the flesh is orange-red, dense and juicy, the fragrance is strong, the sugar content is high, the flavor is excellent, the peel is red, shiny, strong toughness, the fruit is hard, and it is resistant to storage and transportation However, it is not easy to raise seedlings of red strawberry, the detoxification effect of the first generation is poor, pollution and vitrification problems, the germination rate and reproduction coefficient of the first generation of stem tips are low, and the survival rate of transplanting is not high. Abnormal growth of test-tube plantlets or glass plantlets
It shows that the plants are short, the leaves are light and shrunk, and they are crystal-like transparent or translucent water-stained. The stems and leaves become brittle, hard and fragile, which seriously affects the reproduction rate and causes great losses to production.

Method used

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  • Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings
  • Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings
  • Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The experiment of cultivating the shoot tips of strawberry in the primary medium containing different hormones

[0028] The disease-free and robust Japanese rhododendron strawberry seedlings were placed in an artificial climate box, treated at 40 °C under light conditions for 16 hours a day, and the remaining 18 hours at 35 °C under dark conditions, and continued to be treated with variable temperature for 4 weeks; then Obtain terminal buds 2-3cm long, rinse with clean water for 2-3 hours, then disinfect with 75% alcohol for 30s, 5% sodium hypochlorite solution for 10min, and then rinse with sterile water for 5-8 times; 0.5mm-sized shoot apex growth points were inoculated into the above-mentioned primary culture medium, and after 5 days of dark culture, light culture was carried out. The number of inoculated shoot tips for each treatment is 50. The statistics of shoot tip germination rate are based on the cluster buds differentiated 40 days after shoot tip gro...

Embodiment 2

[0032] Example 2 The experiment of cultivating strawberry cluster buds with the subculture medium containing different hormones

[0033] The clustered buds in Example 1 were cut and transferred to the subculture medium for cultivation, the light intensity was 2000lx, the light time was 14h / d, the temperature was 25±2°C, and the subculture generation was controlled at 5-8 generations; At passages 5-6, the macroelement NH in the subculture medium 4 NO 3 The amount of NH is reduced to 1 / 2 of the original; 4 NO 3 The dosage is reduced to 1 / 4 of the original, and other ingredients remain unchanged.

[0034] Subculture sets 3 groups altogether, and each processing inoculates bud number is 50, and subculture medium is formulated as: take MS as basic medium, add hormone (seeing table 2) of different concentrations therein, medium pH5. 7-5.8. 8 generations were subcultured under the above-mentioned medium conditions, and the reproduction coefficients are shown in Table 2.

[0035...

Embodiment 3

[0038] Example 3 Experiment of strawberry seedlings after culturing subculture in rooting medium containing different hormones

[0039] Transfer the subcultured strawberry seedlings to rooting medium to promote rooting, and the number of inoculated seedlings in each treatment is 50, wherein rooting rate %=(rooted seedlings / inoculated seedlings) × 100%, rooting medium The preparation is as follows: take 1 / 2MS (major elements halved, other components unchanged) as the basic medium, add hormones of different concentrations (see Table 3) to it, and the medium pH is 5.7-5.8. The rooting rate was calculated under this medium condition for 30 days, and the results are shown in Table 3.

[0040] Table 3 Rooting media containing different hormones and their rooting rates

[0041]

[0042] The experimental results show that adding auxin IAA, IBA or NAA to the rooting medium can greatly improve the rooting speed and rooting rate, and the effect is NAA>IBA>IAA.

[0043] Put the bottl...

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Abstract

The invention discloses a special medium for tissue culture of strawberry stem tips, belonging to the technical field of tissue culture seedling, comprising a first generation medium, a successive medium and a rooting medium, wherein the first generation medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L and naphthylacetic acid with the concentration of 0.1-0.2mg / L to an MS medium; the successive medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L to an MS medium; and the rooting medium is a 1 / 2 MS medium. The invention also discloses a method for producing detoxification seedlings by using strawberry stem tip tissues cultured by the mediums, comprising the steps of heat treatment of strawberry seedlings, material drawing and sterilization, first generation culture, successive culture, rooting culture, transplanting and the like. The strawberry detoxification fast propagation system of the invention is convenient and practical, and has good detoxification effect, high propagation factor, good genetic stability and high regeneration rate, thus being massively used on commercial purpose production.

Description

technical field [0001] The invention belongs to the technical field of tissue culture seedlings, and in particular relates to a special medium for tissue culture of strawberry shoot tips and a method for producing virus-free seedlings. Background technique [0002] Strawberry (Fragaria ananassa Ducde) is a perennial evergreen herb of the Rosaceae Strawberry genus. It belongs to polyploid and is a kind of crops that rely on stolons for vegetative propagation. The traditional method of seedling cultivation is to adopt stolon propagation. The efficiency is low, which is not conducive to the promotion of excellent varieties, and is highly susceptible to virus infection. There are four kinds of strawberry viruses identified in my country, namely strawberry mottle virus (SMOV), strawberry mild yellow edge virus (SMYEV), strawberry shrunken virus (SCRV) and strawberry veined virus (SVBV). The general infection of seeds and seedlings is a bottleneck problem in strawberry production...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈怀勐郭仰东王立府路河高丽
Owner 北京金六环农业科技有限公司
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