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Cultivation method of non-nucleus one-shell multi-pearl seawater pearls

A pearl and seawater technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of pearl oyster injury, low yield, large wound, etc., and achieve the effect of crystal clear surface, high survival rate, and thick bead layer.

Inactive Publication Date: 2017-12-29
北海市旺海海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the culture of seawater pearls is mainly based on intercalation cultivation. The pearl nucleus is implanted in the pearl oyster through surgery and then placed in a suitable culture environment. Natural cultivation allows the pearl layer secreted by the pearl oyster to wrap the bead nucleus and form a pearl. The pearls formed by inserting the bead nucleus are easy to cause injury to the pearl oyster. The wound is too large, which affects the survival rate. The pearl layer is thinner, the market value is lower, and the output is also low.
And the pearl that forms by implanting the small piece of cell culture, also does not see the report that culture success is arranged in the pearl in the sea, therefore, seek a kind of seawater pearl culture method of seedless one shellfish multi-pearl, for cultivating seedless seawater pearl, reduce It is of great significance to reduce the cost of farming and improve market competitiveness

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) First prepare the dilution of adenosine triphosphate disodium, take a 10 ml of adenosine triphosphate disodium injection with a content of 10 mg, add 50 ml of sterile water and stir evenly to obtain the dilution of adenosine triphosphate disodium; then take an adult pearl oyster as a supply body, washed with normal saline, and the upper and lower shells of the pearl oyster donor were separated by surgery in a clean environment. Rinse with disodium diluent;

[0014] (2) Cut the donated extrapermeable membrane tissue into outer permeable membrane tissue particles with a particle size of 0.1-0.6 mm, and the outer permeable membrane tissue particles and the outer permeable membrane mucus form the outer permeable membrane tissue fluid;

[0015] (3) Take adult healthy seawater pearl oysters as recipients, separate the upper and lower shells of the recipient shells by a gap in a clean environment, inhale the tissue fluid of the outer permeable membrane with a straw, and tr...

Embodiment 2

[0019] Except step (3), all the other steps are identical with embodiment 1;

[0020] (3) Take adult healthy seawater pearl oysters as recipients, separate the upper and lower shells of the recipient shells by a gap in a clean environment, inhale the tissue fluid of the outer permeable membrane with a straw, and transfer the tissue fluid of the outer permeable membrane from the outer permeable membrane of the recipient shellfish through the straw. The amount of outer permeable membrane tissue particles implanted in the second nuclear site of the recipient shellfish is 1-6 particles.

Embodiment 3

[0022] Except step (3), all the other steps are identical with embodiment 1;

[0023] (3) Take adult healthy seawater pearl oysters as recipients, separate the upper and lower shells of the recipient shells by a gap in a clean environment, inhale the tissue fluid of the outer permeable membrane with a straw, and transfer the tissue fluid of the outer permeable membrane from the outer permeable membrane of the recipient shellfish through the straw. Implanted into the third nuclear site of the recipient shellfish, the amount of implanted outer permeable membrane tissue microparticles is 1-6 grains.

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Abstract

The invention discloses a cultivation method of non-nucleus one-shell multi-pearl seawater pearls. The cultivation method mainly comprises the steps that adult pearl oysters are taken as donors and washed clean, and external permeable membrane tissue in pearl oyster meat is taken in a clean environment and washed clean with adenosine disodium triphosphate diluent; the external permeable membrane tissue is cut into particles, and the particles and external permeable membrane slime form external permeable tissue fluid; adult healthy seawater pearl oysters are taken as receptors, a slot is formed in the portion between the upper shell and the lower shell of each receptor oyster in a clean environment, and the external permeable tissue fluid is implanted into the receptor oysters through receptor oyster external permeable membranes; the overall steps are completed within three minutes. The receptor oysters implanted with the external permeable tissue particles are rapidly placed into clean seawater to be bred temporarily, and then are placed into a seawater breeding environment in three hours to be naturally bred. The adenosine disodium triphosphate diluent is prepared with 10 milliliters and 10 milligrams of commodity adenosine disodium triphosphate and 50 milliliters of sterile water. The pearl oysters cultivated by using the method have no surgical wound and are high in survival rate and yield and good in quality.

Description

technical field [0001] The invention relates to a method for cultivating seawater pearls with one shell and multiple pearls, and belongs to the technical field of seawater pearl cultivation. Background technique [0002] At present, the culture of seawater pearls is mainly based on inserting nuclei. The pearl nuclei are implanted in the pearl oyster through surgery and then put into a suitable culture environment. The pearls formed by inserting the bead nucleus are easy to cause injury to the pearl oyster. The wound is too large, which affects the survival rate. The pearl layer is thinner, the market value is lower, and the output is also low. And the pearl that forms by implanting the small piece of cell cultivation, also does not see the report that culture success is arranged in the pearl in the sea, therefore, seeks a kind of seawater pearl cultivation method of seedless one shellfish multi-pearl, for cultivating seedless seawater pearl, reduces It is of great significa...

Claims

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Application Information

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IPC IPC(8): A01K61/56
CPCA01K61/56Y02A40/81
Inventor 宁定凤梁秋萍梁萍萍俞艳阳陈鸿霆梁旺海
Owner 北海市旺海海洋科技有限公司
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