Method for culturing 3D baroque pearls
A culture method and pearl technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of shape, poor aesthetic feeling, narrow application range, lack of added value, etc., and achieve the effect of good shape and aesthetic feeling
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example 1
[0030] Example 1: Cultivation of 3D Baroque pearls using the mantle of the triangular sail clam
[0031] First cut out the mantle tissue cell sheet of the triangular sail mussel, sterilize the cut tissue cell sheet, put it into a petri dish, add an equal amount of culture medium, and then put it into an incubator to cultivate the implanted cell sheet used for cultivating pearls. spare. The culture temperature of the incubator was 25°C and the humidity was 100%. The disinfectants for disinfection of tissue cell sheets were: 1 mg / ml streptomycin, 500 IU / ml penicillin, 100 IU / ml gentamicin and 2 μg / ml nystatin. Culture medium for tissue cell sheet culture: 500 μL medium (10% fetal bovine serum, 900mg / L D-galactose, 300mg / L L-glutamine, 550mg / L sodium pyruvate).
[0032] Select the 1-3-year-old triangular sail mussel as the mother-of-pearl mussel. Before the bead breeding operation, rinse the triangular sail mussels and store them temporarily in a relatively clear culture pond ...
example 2
[0035] Example 2: Cultivation of 3D Baroque pearls by using the visceral mass of the pleated crested mussel
[0036] First cut the mantle tissue cell sheet of the crested crested mussel, put the cut tissue cell sheet into a petri dish, add the same amount of culture medium, and then put it into the incubator to cultivate the implanted cell sheet for pearl cultivation, and set it aside. The cultivation temperature of the incubator is 25°C-28°C, and the humidity is 80%-100%. The disinfectant for tissue cell sheet disinfection was: 1 mg / ml streptomycin, 500 IU / ml penicillin, 100 IU / ml gentamycin and 2 μg / ml nystatin. Culture medium for tissue cell sheet culture: 500 μL medium (10% fetal bovine serum, 900mg / L D-galactose, 300mg / L L-glutamine, 550mg / L sodium pyruvate).
[0037] Select the 1-3 age crested crested clam as the mother-of-pearl mussel. Before the bead-raising operation, rinse the pleated crown clam and store it temporarily in a relatively clear culture pond or river w...
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