Visible urine component quality control product composition and preparation method thereof

A composition and control product technology, applied in the field of urine formed component quality control product composition and its preparation, can solve the problems of few components and can not be used for morphological observation, etc., to achieve easy identification, maintain natural attributes, and good stability period Effect

Active Publication Date: 2011-09-14
上海市临床检验中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the technical problems to be solved by the present invention is to overcome the deficiency that the monitoring components of the current urine formed component quality control product are too

Method used

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  • Visible urine component quality control product composition and preparation method thereof
  • Visible urine component quality control product composition and preparation method thereof
  • Visible urine component quality control product composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Quality Control Products of Urine Formed Components

[0031] 1. Choose a plastic bottle with a volume of about 2 liters, a cap, and no particles, and sterilize it by high-pressure steam for use.

[0032] 2. Preparation of 10% normal saline formaldehyde solution

[0033] Formaldehyde 100mL plus normal saline 900mL and mix well.

[0034] 3. Preparation of PH7.0 Phosphate Buffer

[0035] Na 2 HPO 4 2H 2 O 108.61g+NaH 2 PO4·2H 2 O 60.86g was dissolved, and the volume was adjusted to 1L.

[0036] 4. Preparation of PH7.0 neutral formaldehyde solution

[0037] Add 20g of formaldehyde to the above 3 and mix well.

[0038] 5. Add 10% normal saline formaldehyde solution to urine at a ratio of 1:20 into an autoclaved plastic bottle, and keep urine for 12 hours.

[0039] 6. Collect urine and transfer it according to biosafety requirements.

[0040] 7. After fully mixing the urine, pour it into the prepared Buchner funnel with filter membrane for suction fi...

Embodiment 2

[0046] Repeatability Monitoring of Quality Control Products of Urine Formed Components

[0047] 1. Properly dilute the urine formed component quality control substance prepared in Example 1 with neutral phosphate buffered saline.

[0048] 2. The low concentration of red blood cells is 10-20 cells / μL, the medium concentration is 20-50 cells / μL, and the high concentration is >100 cells / μL;

[0049] The low concentration of white blood cells is 10-20 / μL, the medium concentration is 20-50 / μL, and the high concentration is >100 / μL;

[0050] The low concentration of epithelial cells is 20 cells / μL;

[0051] The low concentration of epithelial cells is 20 cells / μL;

[0052] The low concentration of the cast is 2-3 / μL, the medium concentration is 3-5 / μL, and the high concentration is >5 / μL.

[0053] 3. The measurement was repeated 12 times on the Sysmex UF500 Urine Formed Components Analyzer in Japan, and the respective mean, standard deviation (s) and imprecision (cv%) were calculat...

Embodiment 3

[0058] Urine Formed Component Quality Control Stability Monitoring

[0059] 1. After fully mixing the urine formed component quality control product prepared according to Example 1, divide it into 2 mL tubes.

[0060] 2. Monitor the aliquoted urine formed component quality control product once a week on the Japan Sysmex UF500 urine formed component analyzer. A total of 8 weeks were measured.

[0061] 3. For the time-dependent changes of different parameters of the urine organic quality control products we made, see the regression fitting curve analysis results, see Table 2.

[0062] The results showed no significant change in urine formed components over 8 weeks.

[0063] Table 2 Analysis results of SPSS13.0 regression fitting curve of different parameter monitoring values

[0064]

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Abstract

The invention relates to a visible urine component quality control product composition and a preparation method thereof. The visible urine component quality control product composition provided by the invention at least comprises the components such as red blood cells, white blood cells, epithelial cells and a urinary cylinder. Natural attributes of urine are kept, and the composition has imprecision. The visible urine component quality control product composition has good stable stage, is suitable for visible component analyzers of different detection principles, and is suitable for quality control of visible urine component microscopic examination.

Description

technical field [0001] The invention relates to the field of quality control of clinical humoral examination, in particular to a composition of quality control product of urine formed components and a preparation method thereof. Background technique [0002] Urinalysis is widely used as a routine test item in clinic because of its convenient sampling and no pain for patients. Through the physical and chemical examination of urine, the general properties and chemical components of urine can be observed, and the urine pathology can be identified by observing the formed elements in urine such as red blood cells, white blood cells, epithelial cells, casts, crystals, phagocytes, tumor cells, bacteria, and sperm Element. Urine formed component tests often provide clinicians with essential information about a patient's disease status. [0003] In order to reduce the load of inspectors for microscopic examination of urine formed components, improve the inspection process, reduce e...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N15/00G01N21/00C12Q1/68
Inventor 王青胡晓波季慧峰李泳宋颖李栋成许蕾
Owner 上海市临床检验中心
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