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Ocean actinomycete fermentation extract, composite thereof, and application in biofouling resistance

A technology of marine actinomycetes and extracts, applied in the direction of chemicals, applications, biocides for biological control, etc., can solve the problems of affecting the marine environment, high toxicity of chemicals, low efficiency of physical removal methods, etc.

Active Publication Date: 2009-06-03
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of the physical removal method is too low, and the chemicals used in the chemical removal method are too toxic, which will inevitably affect the surrounding marine environment.

Method used

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  • Ocean actinomycete fermentation extract, composite thereof, and application in biofouling resistance
  • Ocean actinomycete fermentation extract, composite thereof, and application in biofouling resistance
  • Ocean actinomycete fermentation extract, composite thereof, and application in biofouling resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Inoculate marine actinomycetes (Salinispora pacifica) SCSIO 00013 into two sterilized 500mL shake flasks. Starch 10~30g, K 2 HPO 4 0.5~1g, KNO 3 0.5~1.0g, MgSO 4 ·7H 2 O 0.5~1.0g, sea salt 1.5%~3%,), temperature 28 ℃ on the shaker, speed 40 rpm, after culturing for 3 days, transfer to 10 seed bottles respectively, after being in Gaoshi No. 1 medium medium, temperature 28°C, speed 40 rpm, after 7 days of cultivation, transfer the actinomycetes from 20 seed bottles to 500mL shake flasks, and the shake flasks are filled with 150mL Gaoshi No. 1 medium, and the culture conditions are temperature 28°C, speed 40 rpm, culture for 10 days, after a large amount of actinomycetes are fermented, use a large centrifuge to separate mycelia and fermentation broth. The fermentation broth was extracted three times with an equal volume of ethyl acetate, and the extracts were combined to obtain extract 1. The mycelium was ultrasonically treated, and then extracted three times with ...

Embodiment 2

[0021] Embodiment 2: Example 1 extract antibacterial and antifouling biological larva attachment activity test

[0022] Antibacterial activity was determined by the agar diffusion method. A sterile paper piece (5 mmi.d.) soaked with 50 μg of the target extract was placed on the agar plate coated with bacteria, and the size unit of the inhibition zone was expressed in mm. The disc soaked with the same volume of DMSO was used as blank control, and the disc added with rifampicin (3-[[(4-methyl-1-piperazinyl)imino]methyl]-rifamycin) was used as positive control. After incubation at room temperature for 24 hours, the size of the inhibition zone was measured. Antibacterial results are shown in Table 1.

[0023] Table 1 Antibacterial activity of the target extract and rifampicin (the size unit of the inhibition zone L, mm; the content of each tablet, 50 μg / disc)

[0024]

[0025] The size of the inhibition zone L in Table 1 is measured by a vernier caliper, and the inhibition ...

Embodiment 3

[0026] Embodiment 3: Anti-fouling biological larva adhesion test of the extract of embodiment 1

[0027] The anti-barnacle larvae attachment activity of the extracts from Example 1 were measured using 24-well plates. 1 mL of culture solution containing 10-15 mature barnacle larvae was added to each plate well, and the extract from Example 1 was dissolved in DMSO, and then diluted with sterilized seawater to different concentrations. Three parallels were performed for each concentration, and sterile seawater was used as a blank control. The culture plate was cultured at room temperature for 24 hours, and the number of attached larvae was counted under a dissecting microscope, and statistical analysis was performed with SPSS program. The statistical results are shown in Table 2:

[0028] Table 2 The attachment rate (%) of the barnacle larvae at different concentrations of the extract of Example 1

[0029]

[0030] It can be seen from Table 2 that compared with the DMSO contr...

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Abstract

The invention relates to an application of ocean actinomycete (Salinispora pacifica) SCSIO 00013 fermentation extract in biofouling resistnace and is characterized in that the ocean actinomycete SCSIO 00013 stain is inoculated to a Gause 1 cultural medium so as to be fermented; the gained mycelium is separated from fermentation solution and is then extracted by organic solvent; coarse extract is gained by concentrating and combining the extract; the coarse extract is suspended on water and extracted by chloroform or ethyl acetate; chromatography of the extract is carried out by a normal-pressure silica gel column; a solution system such as chloroform-acetone and the like is used as eluent so as to carry out gradient elution; thin layer chromatography analysis of fraction is carried out; chloroform-acetone with the volume ratio of 4:1 is used as solvent to extend the system; the fraction with the Rf value Rf ranging from 0.20 to 0.75 is collected and is then combined; subsequently, the pigment is removed, thus gaining target extract. The fermentation extract can be used for halobios fouling resistance; the coating consisting of the fermentation extract and film-forming material contains no heavy metal, has no harm on the environment and can be suitable for the cultivation of marine pearl oyster.

Description

technical field [0001] The present invention relates to a marine actinomycetes fermented extract and its composition and its application in anti-biofouling, in particular to the extract obtained from the fermentation of marine actinomycetes (Salinispora pacifica) SCSIO 00013 and the extract containing the The composition of the extract and the use of the extract in anti-marine biofouling. Background technique [0002] Underwater facilities in marine aquaculture, such as cages, hulls, fishing nets, buoys, drainage pipes, etc., are often susceptible to pollution from marine organisms such as marine bacteria, marine fungi, marine algae, marine protozoa, and molluscs. These fouling organisms will eventually form fouling biological communities on underwater facilities, thereby increasing the energy consumption and weight of underwater facilities, reducing efficiency and speed, and also clogging pipelines, reducing nutrients and oxygen. transport, and even lead to the death of ma...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P9/00C09D5/16
Inventor 张偲罗雄明尹浩漆淑华田新朋
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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