Extracting and purifying method of high-purity sulforaphane

Abstract

The invention discloses a method for extracting high-purity sulforaphane from broccoli seeds; and the method comprises the following steps of: (1) after crushing dry broccoli seeds, adding water to the dry broccoli seeds to hydrolyze the broccoli seeds, so as to obtain a solid-liquid mixture; (2) adding anhydrous ethanol to the mixture; ultrasonically extracting the mixture; and carrying out suction filtration or double-layer gauze filtering on the mixture; (3) concentrating and evaporating ethanol from a filter liquor in a vacuum state; (4) carrying out chromatography on a material liquor by using a macroporous resin column and eluting the material liquor by using an ethanol solution in a gradient way; (5) collecting an eluting solution containing sulforaphane; carrying out chromatography on the eluting solution by using an alumina column; and concentrating a sulforaphane chromatography solution to form an extract so as to obtain a crude sulforaphane product; (6) adding water to the obtained crude product so as to dissolve the crude product; and extracting the dissolved crude product by using a polar solvent; and (7) collecting an organic layer; and concentrating the organic layer in a vacuum state so as to obtain an oily extract, so that the high-purity sulforaphane is obtained. As the high-purity sulforaphane can be directly extracted from the broccoli seeds and broccoli related materials, the method for extracting the high-purity sulforaphane from the broccoli seeds, disclosed by the invention, has the advantages of simple process, stable product, simple equipment and safety in production and is suitable for enterprises for industrially extracting the sulforaphane.

Description

technical field

[0001] The invention relates to a method for extracting and purifying active ingredients in natural products, in particular to a method for extracting high-purity sulforaphane from broccoli seeds. Background technique

[0002] According to the statistics of the World Health Organization, the number of deaths due to cancer in the world is as high as 7 million every year. But so far, people have not found a way to conquer cancer. Therefore, studying and controlling tumors is a major topic in the field of life sciences today. Epidemiological and pharmacological studies have shown that where the incidence of cancer is low, the proportion of vegetables and fruits in people's diet is relatively large. The reason is that there are one or more active ingredients in vegetables and fruits that prevent or slow down the development of cancer, such as lycopene in tomatoes, soy isoflavones in soybean products, tannic acid in berries, and cruciferous fruits and vegetables...