Method for cultivating pearls in test tube

A cultivation method and test-tube technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of low degree of application and industrialization, inability to increase added value, and restrict the sustainable and healthy development of the pearl industry. quality effect

Inactive Publication Date: 2014-07-23
JIANGXI WANNIAN FENGZHU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although my country has an annual pearl output of 1200-1500 tons, the output of high-value-added high-quality pearls, that is, raw pearls that can be processed into jewelry, is not high, and there are nearly 900 tons of low-value-added inferior pearls every year. Technical limitations, low application and industrialization levels, and inability to increase added value have severely restricted the sustainable and healthy development of the domestic pearl industry.

Method used

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  • Method for cultivating pearls in test tube

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Effect test

Embodiment 1

[0020] Such as figure 1 As shown, a method for cultivating test-tube pearls: first, select 1-year-old chrysalis juveniles, cut off the marginal membrane of their mantle, peel off the outer skin of the marginal membrane by tearing the film, wash and disinfect, and cut into 0.5 mm-sized cell fragments; then, put the cell fragments into a culture flask, add trypsin digestion solution with a concentration of 0.3 parts (parts by weight) and a pH value of 7.2, and place in a constant temperature incubator at a temperature of 25°C Digest for 15 minutes, filter with 200-mesh silk net to remove excess tissue pieces, add a small amount of inactivated and sterilized body fluid of chrysalis to make epithelial cell fluid; secondly, use RPMI1640 basic medium with a total volume of 45 parts (by weight) , 30 parts of total volume (parts by weight), calf serum of 15 parts (parts by weight) of total volume, and an appropriate amount of penicillin and streptomycin are prepared into a complete m...

Embodiment 2

[0022] A method for cultivating test-tube pearls: first, select 1-year-old chrysalis juveniles, cut off the marginal membrane of their mantle, peel off the outer skin of the marginal membrane by tearing the membrane, wash and disinfect, and cut into cells with a size of 1 mm. Small pieces; then, put the small pieces of cells into culture flasks, add trypsin digestion solution with a concentration of 0.3 parts (parts by weight) and a pH value of 7.2, and digest them in a constant temperature incubator at 25°C for 20min. 200 mesh tulle filter to remove excess tissue pieces, add a small amount of inactivated and disinfected body fluid of the chrysalis to make epithelial cell fluid; secondly, use 50 parts (by weight) of RPMI1640 basic medium, 40 parts (by weight) ), 20 parts (by weight) of calf serum and appropriate amount of penicillin and streptomycin to prepare a complete culture medium, so that the concentration of penicillin and streptomycin is 100IU / ml; again, the prepared P...

Embodiment 3

[0024] A method for cultivating test-tube pearls: first, select 1-year-old chrysalis juveniles, cut off the marginal membrane of their mantle, peel off the outer skin of the marginal membrane by tearing the membrane, wash and disinfect, and cut into 0.5mm-sized pearls. Small pieces of cells; then, put the small pieces of cells into culture flasks, add trypsin digestion solution with a concentration of 0.3 parts (parts by weight) and a pH value of 7.2, and digest them in a constant temperature incubator at 25°C for 15min. Use 200 mesh silk screen to filter and remove excess tissue pieces, add a small amount of inactivated and sterilized mussel body fluid to make epithelial cell fluid; secondly, use 55 parts (by weight) of RPMI1640 basic medium, 50 parts (by weight) Parts) of the body fluid of the chrysalis, 25 parts (by weight) of calf serum and an appropriate amount of penicillin and streptomycin to prepare a complete medium, so that the concentrations of penicillin and strepto...

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Abstract

The invention discloses a method for cultivating pearls in a test tube. The method includes the first step of getting down the exocuticle of a margin membrane of the pallium of a baby clam in a membrane stripping mode, the second step of digesting the exocuticle to form epithelium cell sap, the third step of preparing a culture medium, the fourth step of carrying out cell culture to enable cells to be attached to the nucleus to form a complete pearl cultivation sac, and the fifth step of implanting the pearl cultivation sac into the body of a pearl cultivation clam for pearl cultivation. According to the method, pallium cells are cultivated in the test tube, the cells are evenly attached to the pearl nucleus to form the complete pearl cultivation sac, and then the pearl cultivation sac is implanted into the body of the pearl cultivation clam for pearl cultivation; the probability that in the prior art, the small pallium can not grow evenly to form the complete pearl cultivation sac due to operation errors or other reasons, so that nacre secreted by the pearl cultivation sac is not uniform, and bad-quality pearls such as dirty pearls, tailed pearls and pearls with burnt heads are produced is reduced, and the quality of the cultured pearls is effectively improved.

Description

technical field [0001] The invention relates to the field of freshwater pearl cultivation, in particular to a method for cultivating test tube pearls. Background technique [0002] At present, the output of my country's artificially cultured freshwater pearls has ranked first in the world, from more than 1,000 tons of pearls produced in 2000 (accounting for 95 parts (weight parts) of the world's total pearl production) to more than 1,200 tons of pearls produced in 2005 (accounting for about 1,000 tons of the world's total). 90 parts (by weight) of the output), its output reached a peak in 2008, about 1500t, and is currently maintained at 1200-1500t. Although the output of pearls has increased, the quality of pearls has not improved significantly. Of the freshwater pearls produced in my country, less than 20% are high-quality and high-grade pearls. A large number of low-quality pearls are flooding the market, leading to a drop in pearl prices, the pearl market continues to b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K61/56
CPCY02A40/81
Inventor 丁文军曹士广齐玉平陈莉钟瑞王美红
Owner JIANGXI WANNIAN FENGZHU IND
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