Sea no-nucleus pearl incubation method

A technology of non-nuclear pearls and seawater, applied in biochemical equipment and methods, tissue culture, climate change adaptation, etc., can solve the problems of large damage to pearl oysters, low success rate, and weak attachment of pearl sacs, and achieve operational Simple, less chance of contamination, high survival rate and beading rate

Active Publication Date: 2009-07-29
广西中医学院 +1
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AI Technical Summary

Problems solved by technology

At the same time, the connective tissue cells in the mantle tissue are wrapped on the surface of the outer epithelial cells, and finally form a pearl sac with a relatively complete struct

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Weigh the papain according to the proportion, dissolve it in three-distilled water to prepare a papain solution with a mass content of 0.5%, mix well, filter and sterilize with a plate and frame filter, and set aside at 4°C. Take out the mantle epithelial tissue of 1-2 years old healthy Pinctada martensii according to the conventional method, remove the crony part, cut it into as small pieces as possible after purification treatment; put it in papain solution for digestion, the temperature is 20℃-25℃ ℃.

[0033] (2) Quickly extract oyster tissue fluid and oyster serum from the above-mentioned oyster body that provides the mantle epithelial tissue (take blood from the oyster heart or a large blood vessel, centrifuge, and get the supernatant to be the oyster serum); make a ratio For: shellfish tissue fluid: shellfish serum: sterilized filtered seawater = 1~1.5: 0.5~1:8 culture solution, add 1.5ml of 100IU / ml penicillin and 1.5ml of 0.1g / L streptomycin to every 100ml c...

Embodiment 2

[0038] (1) Weigh trypsin and dissolve it in 50ml of three-distilled water to prepare a 0.3% trypsin solution, mix well, filter and sterilize with a microporous membrane, and reserve at 4°C; take out 1-2-year-old healthy black The mantle epithelial tissue of Pinctada oyster oyster, remove the crony part, cut into as small pieces as possible after purification; digest in trypsin solution, the temperature is 20°C-25°C.

[0039] (2) Shellfish serum was prepared from the same species of pinnacle oyster or from other seawater shellfish and stored in a -20°C refrigerator. Melt at 4°C before use, use as soon as possible, and do not need to be inactivated in a water bath before use. Quickly extract shellfish tissue fluid and shellfish serum melted at 4°C from the above-mentioned pinnacle shells that provide the mantle epithelial tissue; the ratio is: shellfish tissue fluid: shellfish serum: sterile filtered seawater=1~1.5:0.5~1:8 When in use, add 2ml of 100IU / ml penicillin and 2ml of ...

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PUM

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Abstract

The invention discloses a method for artificially culturing seawater anucleate pearl. The method comprises the following steps: taking out the mantle epithelium of a healthy nacre, removing the skirt band part, digesting the mantle epithelium in protease solution, then adding the culture solution with shell blood serum to stop the digestion, culturing the culture solution containing shell tissue fluid and a property amount of penicillin and streptomycin in a sterile environment for 5 to 30 hours, then injecting the cell suspension into a pearl breeding shell body, and putting the pearl breeding shell body in the seawater for suspended culture or into a culture pond box for feeding after a recreation period. The method has little damage to the pearl breeding shell, a high survival rate and a high pearl formation rate. The cultured pearl is suitable for medicinal use. The method has convenient operation and low cost.

Description

technical field [0001] The invention relates to a pearl cultivation technology, in particular to a seawater seedless pearl cultivation technology for medicinal use. Background technique [0002] Natural pearl resources are scarce. In the natural environment, only more than 10 grams of pearls can be collected from every 10,000 mother oysters, and the particles are small and poor in shape. In addition, collecting pearls is a very dangerous and difficult job. With the rise of the Pearl River Delta, the pearl fishing industry has also disappeared, so the current sales on the market are all cultured pearls. [0003] Like natural pearls, cultured pearls are divided into seawater pearls and freshwater pearls. Artificial seawater cultured pearls are mainly concentrated in coastal areas such as Hepu, Lianjiang and Hainan Island in Guangxi and Guangdong. Hepu pearls are rich in nutrients and various life-source substances, and have been the first choice for traditional Chinese medici...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12N5/06
CPCY02A40/81
Inventor 林江林湧邓家刚王乃平王勤甘霖单华林海林其溪杨辉戴铭李中华刘强陈明伟杨继峰
Owner 广西中医学院
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