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Pearl nucleus having mantellum cell hydrogel layer at surface and preparation method therefor

A technology of mantle cells and hydrogel layer, which is applied in the field of pearl core and its production, can solve the problems of cell damage and shedding, single cell type, and spherical pearl core is easy to roll, so as to improve compatibility, improve economic benefits, Pearly uniform effect

Inactive Publication Date: 2016-01-27
肖义军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following problems: (1) Normal epidermal cells can only adhere to the wall and grow into a single layer under artificial culture conditions, the amount of cells is too small, and the cell type is single
(3) The spherical pearl nucleus is easy to roll, and it is easy to cause cell damage and fall off due to collisions, etc. It is difficult to ensure that the cells are not damaged during transportation, nucleus implantation, etc.
Therefore, it has not been applied in production practice so far.

Method used

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  • Pearl nucleus having mantellum cell hydrogel layer at surface and preparation method therefor
  • Pearl nucleus having mantellum cell hydrogel layer at surface and preparation method therefor
  • Pearl nucleus having mantellum cell hydrogel layer at surface and preparation method therefor

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Effect test

Embodiment 1

[0026] 1. The polished common pearl nucleus is autoclaved, soaked in 0.01wt% polylysine solution for 30 minutes, taken out, and dried under aseptic conditions.

[0027] 2. Preparation of pearl core hydrogel layer mold: adopt secondary molding method. Mold Ⅰ is composed of two hollow hemispheres with the same structure, and when used, the two hemispheres are closed to form a complete sphere. On the inner surface of each hemisphere, there are 3 strip-shaped protrusions (hereinafter referred to as ribs) arranged in the direction of the north and south poles (the location of the glue injection hole is the south pole, and the opposite side is the north pole). Two of the ribs are located on the edges of the hemisphere ( Hereinafter referred to as the edge edge 3), and the other edge is located in the middle of the hemisphere (hereinafter referred to as the middle edge 2) ( figure 1 , figure 2 ), the length of the ribs is 1 / 2 of the circumference of the sphere, so the ends of the ...

Embodiment 2

[0031] 1. The polished pearl nucleus is sterilized by high pressure, put into 0.01wt% polylysine solution and shaken for 30 minutes, then taken out and dried under aseptic conditions.

[0032] 2. Preparation of pearl core hydrogel layer-by-layer mold: adopt one-shot molding method. The mold consists of two hemispheres ( Figure 5 , Figure 6 ), there are 3 thin columns 7 perpendicular to the plane of the hemisphere on the inner surface of each hemisphere, which are at a position 120° apart from each other. Make the thickness of the hydrogel layer uniform and perpendicular to the plane of the hemisphere so that the hydrogel layer will not be damaged during demoulding. The length of the thin column is about 1.2 mm. After the two hemispheres are closed, there is a small hole 6 on the spherical surface for perfusing the hydrogel solution. When the poured hydrogel cell suspension cross-links and coagulates into a gel, it is released from the mold. Since the polymer material in ...

Embodiment 3

[0035] Embodiment 3: Collagen of shellfish is used as the raw material of polymer hydrogel.

[0036] 1. Preparation of Hydrangea Collagen:

[0037] ⑴Remove the viscera from the beaded clams, wash them, dry them, cut them into small pieces, and freeze-dry them;

[0038] (2) Take the freeze-dried mussel meat and add 0.1M NaOH, the ratio is 1:30 (w / v), mash it with a tissue masher, and mash it at 4°C for 24 hours;

[0039] (3) Refrigerate and centrifuge the clam tissue treated with NaOH at 4°C (12000r / min, 30 minutes), take the precipitate, wash it thoroughly with ultrapure water until the supernatant is neutral or slightly alkaline, and centrifuge to get the precipitate;

[0040] (4) Soak the precipitate in 10vol% n-butanol for 24 hours, with a volume ratio of 1:30 (v / v);

[0041] (5) Freeze and centrifuge the clam tissue treated with n-butanol at 4°C, take the precipitate, and freeze-dry;

[0042] ⑹ Wash the material fully with deionized water, then soak in 0.5M acetic acid ...

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Abstract

The invention discloses a pearl nucleus having a mantellum cell hydrogel layer at the surface and a preparation method therefor, and belongs to the field of production of artificial cultured pearls. The preparation method comprises separating mantellum cells of a pearl mussel into individual free cells through enzymolysis, premixing the individual free cells in a high-molecular aqueous solution, and making the high-molecular aqueous solution gel on the surface of a pearl nucleus to form hydrogel containing mantellum cells and being almost uniform in thickness. By adopting the preparation method, the mantellum cells can be almost uniformly distributed at the periphery of the pearl nucleus, the problem that mantellum cuticle cells are not uniform in distribution on the periphery of a pearl nucleus by adopting a traditional method of implantation of small particle of mantellum cells can be solved.

Description

technical field [0001] The present invention relates to a pearl nucleus and a production method thereof, in particular to a pearl whose surface is covered with a hydrogel layer containing shellfish animal mantle epidermal cells and formed by cross-linking of biodegradable polymer materials Nuclei and methods for their preparation. Background technique [0002] At present, the artificial culture method of nucleated pearls usually adopts the small pieces of mantle cells taken from donor mussels and the pearl nuclei prepared from shells to be implanted into the nuclei of pearl mussels, and then put into the breeding site to cultivate and cultivate pearls. After a period of breeding, the mussels are killed to get the pearls. In the pearl-growing mussel, the epidermal cells freed from the mantle cells will gradually migrate to the surface of the pearl nucleus, and some other types of cells, such as connective tissue cells, surround the outer skin cells and connect with the conne...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/56Y02A40/81
Inventor 肖宽诚肖义军
Owner 肖义军
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