Preparation method of molecular imprinting polymer with specific recognition capability to tetracycline family

A recognition ability and molecular imprinting technology, applied in chemical instruments and methods, other chemical processes, etc., to achieve high sensitivity, good reproducibility, and simple operation

Inactive Publication Date: 2010-10-13
HUBEI INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecularly imprinted polymers that only specifically recognize tetracyclines are difficult to meet the needs of practical work. It is urgent to develop a molecularly imprinted polymer that can specifically recognize tetracyclines, and realize specific recognition of 10 tetracycline antibiotics.

Method used

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  • Preparation method of molecular imprinting polymer with specific recognition capability to tetracycline family
  • Preparation method of molecular imprinting polymer with specific recognition capability to tetracycline family
  • Preparation method of molecular imprinting polymer with specific recognition capability to tetracycline family

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Dissolve 1 mmol aureomycin and 1 mmol oxytetracycline as template molecules with 8 mmol functional monomer methacrylic acid in 2 mL containing 40 mmol cross-linking agent N, N'-methylenebisacrylamide and 0.08 mmol initiator azobisiso Prepare a mixed solution in acetonitrile of nitrile; ultrasonically degas the mixed solution for 6 minutes, pass nitrogen gas for 5 minutes to remove oxygen molecules, then seal the mixed solution, and polymerize for 18 hours under stirring at 50°C to form a white powdery imprinted polymer. After the polymerization is completed, use 100 mL of a mixed solution of acetic acid and methanol (wherein the volume percentage concentration of acetic acid is 10%), and use Soxhlet extraction to extract the above-mentioned white powdery imprinted polymer to remove template molecules, and then use methanol Soxhlet extraction method was used to extract and remove acetic acid molecules. After the extraction, the polymer was dried in a vacuum drying oven t...

Embodiment 2

[0028]Dissolve 1mmol aureomycin and 1mmol oxytetracycline as template molecules with 2mmol functional monomer methacrylic acid to 2mL containing 2mmol N,N'-methylenebisacrylamide and 0.02mmol initiator azobisisobutyronitrile Prepare a mixed solution in acetonitrile; the mixed solution was ultrasonically degassed for 5 minutes, nitrogen gas was passed for 10 minutes to remove oxygen molecules, and then the mixed solution was sealed and polymerized for 12 hours under stirring at 45°C to produce a white powdery imprinted polymer. After the polymerization is completed, use 100 mL of a mixed solution of acetic acid and methanol (wherein the volume percentage concentration of acetic acid is 10%), and use Soxhlet extraction to extract the above-mentioned white powdery imprinted polymer to remove template molecules, and then use methanol Extraction removes acetic acid molecules. After the extraction, the polymer was dried in a vacuum drying oven to a constant weight, and finally a mol...

Embodiment 3

[0030] Dissolve 1mmol aureomycin and 1mmol oxytetracycline as a template molecule with 20mmol functional monomer methacrylic acid in 20mL containing 200mmol N,N′-methylenebisacrylamide and 2mmol initiator azobisisobutyronitrile Prepare a mixed solution in acetonitrile; ultrasonically degas the mixed solution for 10 minutes, pass nitrogen gas for 10 minutes to remove oxygen molecules, then seal the mixed solution, and polymerize for 24 hours under stirring at 60°C to generate a white powdery imprinted polymer. After the polymerization is completed, use 100 mL of a mixed solution of acetic acid and methanol (wherein the volume percentage concentration of acetic acid is 20%), and use Soxhlet extraction to extract the above-mentioned white powdery imprinted polymer to remove template molecules, and then use methanol Extraction removes acetic acid molecules. After the extraction, the polymer was dried in a vacuum drying oven to a constant weight, and finally a molecularly imprinted...

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Abstract

The invention discloses a preparation method of a molecular imprinting polymer with specific recognition capability to a tetracycline family. The method comprises the following steps of: dissolving chlortetracycline, oxytetracycline chlortetracycline, functional monomers, a cross-linking agent and an initiating agent in acetonitrile by a certain proportions, mixing, then sealing and carrying out polymerization; carrying out extraction on generated polymer by firstly using a mixture solution of methanol and acetic acid to remove template molecules and then using methanol to extract and remove the acetic acid, and finally carrying out vacuum drying. The method is simple in operation; and the prepared molecular imprinting polymer has specific recognition capability to the tetracycline family, can carry out enriched purification on tetracycline antibiotic residues in a sample with speediness, sensitivity, accuracy and high efficiency and has the advantages of high recovery ratio, good reproducibility and high sensitivity.

Description

technical field [0001] The invention relates to a preparation method of a molecularly imprinted polymer with specific recognition ability for tetracyclines, which belongs to the field of new materials. Background technique [0002] Tetracyclines are a class of basic broad-spectrum antibiotics produced by Streptomyces or semi-synthesized. They can be used to prevent poultry and livestock diseases and promote the growth of young poultry and livestock, so they are widely used as veterinary drugs. However, unreasonable use of this type of drug, such as excessive dosage, long duration of drug use, drug abuse, and early slaughter without observing the drug withdrawal period, will cause this type of drug and its metabolites to remain in the muscles, eggs, and milk of animals. , In organ tissues. After humans ingest food containing tetracycline antibiotic residues for a long time, the drug will continue to accumulate in the body. When the concentration reaches a certain amount, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/38C08F226/06C08F220/06C08J9/26B01J20/285B01J20/30
Inventor 王鹏荆涛胡小钟
Owner HUBEI INSPECTION & QUARANTINE TECH CENT
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