Preparation method of molecularly imprinted polymer capable of enriching hispidin

A technology of molecular imprinting and polymer, applied in chemical instruments and methods, other chemical processes, etc., can solve the problem of limited natural sources of hard hairin, and achieve the effect of rapid enrichment and purification, easy implementation, and simple process

Active Publication Date: 2014-11-05
NANKAI UNIV
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Problems solved by technology

However, the natural sources of hardwood reported so far are limited, mainly from the mushrooms of the genus Acupunculus and Acupunculus, such as Phellinus igniarius, Inonotus obliquus, Phellinus obliquus, Phaeoporus obliquus, etc.

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  • Preparation method of molecularly imprinted polymer capable of enriching hispidin

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Embodiment

[0022] A method for preparing a molecularly imprinted polymer that can be enriched for cleillin, the steps are as follows:

[0023] 1) Dissolve 61.5mg of stearin and 88.85mg of acrylamide in a mixed solvent of 10mL of tetrahydrofuran and 2mL of isooctane, mix well and stir for 2h;

[0024] 2) Add 1647μL ethylene glycol dimethacrylate, mix well and stir for 10min;

[0025] 3) Add 30 mg of azobisisobutyronitrile, mix well and stir for 10 minutes to obtain a mixed solution;

[0026] 4) Deoxidize the above mixture by blowing nitrogen for 4 minutes, seal it and place it in a constant temperature water bath for 72 hours at 60°C to obtain a block polymer;

[0027] 5) Grind the above-mentioned massive polymer with a mortar, screen with a grading sieve and retain the polymer particles of 60-100 mesh, and then extract the polymer particles with 250mL Soxhlet extract to remove unreacted substances, The Soxhlet extract is a mixture of methanol and acetic acid with a volume ratio of 9:1,...

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Abstract

The invention relates to a preparation method of a molecularly imprinted polymer capable of enriching hispidin, which comprises the following steps: by using hispidin as a template molecule, a tetrahydrofuran-isooctane mixed solvent as a pore-forming agent, acrylamide as a functional monomer, ethylene glycol dimethyl acrylate as a crosslinking agent and azodiisobutyronitrile as an initiator, evenly mixing, carrying nitrogen-blowing deoxidization, carrying out thermostatic water bath reaction, carrying out Soxhlet extraction, and drying to obtain the molecularly imprinted polymer. The preparation method has the advantages of simple technique and low cost, and is easy to implement; and the prepared molecularly imprinted polymer can be used for separating and extracting hispidin from natural products, has the advantages of high specificity and long service life, and especially can implement quick enrichment and purification for some natural products with very low hispidin content.

Description

technical field [0001] The invention relates to a molecularly imprinted polymer, in particular to a preparation method of a molecularly imprinted polymer that can be enriched in sclerosin. Background technique [0002] Hispidin is one of the active ingredients of various medicinal fungi, and has biological activities such as anti-oxidation, anti-tumor cell proliferation, inhibition of HIV-1 replication, and anti-senile dementia. Studies have found that sclerosin has a strong inhibitory effect on protein kinase C, which may be one of the reasons why it induces tumor cell apoptosis. However, the natural sources of hardwood reported so far are limited, mainly from the mushrooms of the genus Acupunculus and Acupunculus, such as Phellinus igniarius, Inonotus obliquus, Phellinus obliquus, Pore ​​bacteria (Phaeoporus obliquus) and so on. Therefore, it is of great practical significance to further develop natural resources containing sclerosin. [0003] Molecular imprinting techn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F220/56C08F2/00C08J9/28C08J9/26B01J20/26B01J20/30
Inventor 刘方李宁王筱滢赵蕊丽张业尼周蓉
Owner NANKAI UNIV
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