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A preparation method of molecularly imprinted polymers capable of enriching paclitaxel

A technology of molecular imprinting and paclitaxel, applied in chemical instruments and methods, ion exchange, ion exchange regeneration, etc., can solve the problems of difficult separation and purification, high cost, low content, etc., and achieve rapid enrichment and purification, long service life, The effect of simple process

Active Publication Date: 2016-08-17
NANKAI UNIV
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Problems solved by technology

However, due to its low content in nature, difficulty in obtaining, and high cost, its clinical application is limited; at the same time, due to the existence of structurally similar isomers of paclitaxel, it is extremely difficult to separate and purify; paclitaxel itself is also sensitive to heat and easily hydrolyzed. a series of questions

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  • A preparation method of molecularly imprinted polymers capable of enriching paclitaxel
  • A preparation method of molecularly imprinted polymers capable of enriching paclitaxel

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Embodiment

[0018] A preparation method of a molecularly imprinted polymer capable of enriching paclitaxel, using paclitaxel as a template molecule, using acetone solvent as a porogen, using methacrylic acid as a functional monomer, and using ethylene glycol dimethacrylate as a crosslink Agent, with azobisisobutyronitrile as initiator preparation, the steps are as follows:

[0019] 1) Dissolve 860 mg of paclitaxel and 50.8 μL of acrylamide in 7 mL of acetone solvent, mix and stir for 2 h, then add 570 μL of ethylene glycol dimethacrylate, mix and stir for 30 min, then add 30 mg of azo Diisobutyronitrile, mixed and stirred for 30 min to obtain a mixed solution;

[0020] 2) Blow off the above mixed liquid nitrogen to deoxidize for 4 minutes, seal it, and put it in a water bath at a constant temperature of 65°C for 48 hours to obtain a block polymer;

[0021] 3) Dry the above block polymer and grind it, screen it with a grading sieve and keep the 30-60 mesh polymer particles, then add the p...

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Abstract

The invention provides a preparation method of a molecularly imprinted polymer capable of enriching paclitaxel. The molecularly imprinted polymer is prepared by taking the paclitaxel as a template molecule, an acetone solvent as a pore-foaming agent, methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linking agent and azodiisobutyronitrile as an initiator. The preparation method comprises the following steps: dissolving the paclitaxel and methacrylic acid in the acetone solvent, and then adding the ethylene glycol dimethacrylate and the azodiisobutyronitrile; blowing nitrogen into the mixed solution for deoxidization and then sealing, carrying out constant-temperature water bath to obtain a blocky polymer; and drying, grinding and screening the blocky polymer and then adding the polymer to the mixed liquid of methanol and acetic acid, removing the unreacted materials by shaking, and drying to obtain the target object. The method is simple in process, low in cost and easy to implement; the prepared molecularly imprinted polymer can be applied to separating and enriching the paclitaxel from a taxus chinensis crude extract, and is high in specificity and long in service life; the method is capable of realizing quick enrichment and purification and has an industrial production value.

Description

technical field [0001] The invention relates to molecularly imprinted polymers, in particular to a method for preparing molecularly imprinted polymers that can be enriched with paclitaxel. Background technique [0002] Paclitaxel is a kind of diterpenoid compound, which is an anticancer natural drug with high efficiency, low toxicity, broad spectrum, strong activity and unique mechanism of action. Especially for uterine cancer, ovarian cancer, breast cancer has a special effect, can also be used for the treatment of lung cancer, esophageal cancer, reproductive tissue tumors, endometrial cancer, lymphoma, bladder cancer and so on. However, due to its low content in nature, difficulty in obtaining, and high cost, its clinical application is limited; at the same time, due to the existence of isomers with similar structures in paclitaxel, it is extremely difficult to separate and purify; paclitaxel itself is also sensitive to heat and easily hydrolyzed. And so on a series of qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F220/06C08J9/26B01J20/285B01J20/30B01D15/08C07D305/14
Inventor 刘方闫艳李宁周蓉王一君张可人
Owner NANKAI UNIV
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