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Preparation method and application of nano fiber molecularly-imprinted polymer of tetracycline antibiotics

A nanofiber and tetracycline-based technology is applied in the field of nanofiber molecularly imprinted polymer preparation, and can solve problems such as the preparation method and application of nanofiber molecularly imprinted polymers that endanger human health, no tetracycline antibiotics are disclosed, and tetracycline drug residues. , to achieve the effect of improving the detection specificity

Inactive Publication Date: 2015-05-06
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tetracycline Antibiotics are spectrum-type antibacterial drugs, which are often used to prevent and treat livestock and poultry diseases. However, due to the irrational use of drugs, there are often residues of tetracyclines in animal source foods, which endanger human health.
At present, there are no relevant research reports on the preparation method and application of nanofiber molecularly imprinted polymers of tetracycline antibiotics at home and abroad.

Method used

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  • Preparation method and application of nano fiber molecularly-imprinted polymer of tetracycline antibiotics
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  • Preparation method and application of nano fiber molecularly-imprinted polymer of tetracycline antibiotics

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Experimental program
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Effect test

specific Embodiment 1

[0038] A nanofiber molecule is used as a support carrier, and its surface is silanized, doxycycline is used as a template molecule, acetonitrile / methanol is used as a porogen, methacrylic acid (MAA) is used as a functional monomer, trimethylolpropane trimethylolpropane Using methacrylate (TRIM) as a crosslinking agent and azobisisobutyronitrile (AIBN) as an initiator to synthesize nanocellulose molecularly imprinted polymers, using this polymer as an adsorbent, solid-phase extraction-liquid phase Chromatography combined technology to detect residues of tetracycline antibiotics in food.

specific Embodiment 2

[0039] The specific preparation method of the nanocellulose molecularly imprinted polymer of tetracycline antibiotics in the above-mentioned embodiment 1, the experimental materials are as follows:

[0040] Doxycycline (Aladdin Reagent Co., Ltd.); tetracycline (Aladdin Reagent Co., Ltd.); oxytetracycline (Aladdin Reagent Co., Ltd.); Reagent Co., Ltd.); anhydrous lithium chloride (Shanghai Titan Technology Co., Ltd.); N, N-dimethylacetamide (DMAC, Aladdin Reagent Co., Ltd.); 3-mercaptopropyltrimethoxysilane (MPTS, Sinopharm Chemical Reagent Co., Ltd.); methacrylic acid (MAA, Sinopharm Chemical Reagent Co., Ltd.); trimethylolpropane trimethacrylate (TRIM, Maya Reagent Co., Ltd.); azobisisobutyronitrile (AIBN , Tianjin Guangfu Fine Chemical Research Institute); tetraethyl orthosilicate (TEOS, Shanghai Titan Technology Co., Ltd.); sodium dihydrogen phosphate (NaH 2 PO 4 2H 2 O, Yongda Chemical Reagent Co., Ltd.); disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O, Yongda Chemic...

specific Embodiment 3

[0054] The adsorption test, calibration and detection limit of nanofiber molecularly imprinted polymers, the specific steps are as follows:

[0055] (1) HPLC conditions: Chromatographic column: ZORBAX Eclipse XDB-C18, 4.6×150mm, 5μm, Agilent; mobile phase: 0.01mol / L sodium dihydrogen phosphate (pH 2.8): acetonitrile=79:21; detection wavelength : 355nm; flow rate: 0.8mL / min; injection volume: 20μL; column temperature: 30℃, such as Figure 4 It is the liquid chromatogram of the standard sample of tetracycline antibiotics. Under the above-mentioned liquid chromatography experimental conditions, the three tetracycline antibiotics can be analyzed within 15 minutes, and the peaks of tetracycline and oxytetracycline can emerge within the first 7 minutes. The element peaked before 12min;

[0056](2) To make a standard curve: use methanol as a solvent, prepare tetracycline and oxytetracycline at a concentration of 2.5 μg / mL, 5.0 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL, and 100 μg / mL at a ...

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Abstract

The invention discloses a preparation method and application of a nano fiber molecularly-imprinted polymer. The preparation method is characterized by comprising the following steps: carrying out silanization treatment and denaturation on nano fiber, and synthesizing the nano cellulose molecularly-imprinted polymer by using doxycycline as template molecules, acetonitrile / methanol as a pore-forming agent, methacrylic acid as a functional monomer, trimethylolpropane trimethyl acrylate as a crosslinking agent and azodiisobutyronitrile as an initiator. The application method comprises the following steps: characterizing the synthesized polymer material, filling in a solid-phase extraction column, and determining the concentration of tetracycline antibiotics in the sample to be detected according to the standard curve in combination with high-performance liquid chromatography. The application method has the advantages of high sensitivity, high detection speed, low detection limit, high efficiency and low price.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a preparation method and application of a nanofiber molecularly imprinted polymer for detecting residues of tetracycline antibiotics in food. Background technique [0002] Tetracycline Antibiotics are spectrum-type antibacterial drugs, which are often used to prevent and treat diseases in livestock and poultry. However, due to the irrational use of drugs, there are often residues of tetracyclines in animal source foods, which endanger human health. Commonly used tetracycline antibiotics mainly include Tetracycline (Tetracycline, TC), Chlortetracycline (CTC), Oxytetracycline (Oxytetracycline, OTC), etc. Some antibiotics can also be metabolized into epitetracyclines in animals. , not only lead to a decrease in efficacy, but also an increase in its toxic and side effects. The U.S. Food and Drug Administration stipulates that the maximum residues of the above three tetracycline ...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/06C08J9/26B01J20/285G01N1/34
Inventor 潘道东王雅群吴振孙杨赢曾小群曹锦轩
Owner NINGBO UNIV
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