High-sensitivity-ratio fluorescent probe based on composite system of cadmium telluride quantum dots and europium ions and preparation method and application of high-sensitivity-ratio fluorescent probe

A technology of cadmium telluride quantum dots and ratio fluorescent probes, applied in the fields of chemistry and nanomaterials science, can solve problems such as non-reproducibility, and achieve the effects of good selectivity, simple raw materials, and good anti-interference.

Active Publication Date: 2019-05-24
中科合肥智慧农业谷有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these tetracycline fluorescent probes are all single-emission fluorescence sensors, and the measurement only depends on the change of the emission intensity of europium ions. The fluorescence intensity is greatly affected by environmental conditions, instrument efficiency and excitation factors, and this change is often not significant. high repeatability

Method used

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  • High-sensitivity-ratio fluorescent probe based on composite system of cadmium telluride quantum dots and europium ions and preparation method and application of high-sensitivity-ratio fluorescent probe
  • High-sensitivity-ratio fluorescent probe based on composite system of cadmium telluride quantum dots and europium ions and preparation method and application of high-sensitivity-ratio fluorescent probe
  • High-sensitivity-ratio fluorescent probe based on composite system of cadmium telluride quantum dots and europium ions and preparation method and application of high-sensitivity-ratio fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Preparation of cadmium telluride quantum dot solution

[0040] Combine 0.0319g tellurium powder and 0.05g NaBH 4 Mix it in 2mL ultrapure water, and continue to introduce nitrogen to maintain an oxygen-free environment. The solution was stirred in an ice bath and reacted for 10 hours. The mixed solution gradually changed from black to white, and NaHTe was formed in the upper layer. Dissolve 0.2284g of cadmium chloride and 220μL of mercaptopropionic acid in 120mL of ultrapure water, adjust the pH to 9 with 1M NaOH solution, then bubbling with nitrogen for 30 minutes to remove oxygen in the solution. Dilute sulfuric acid was injected into NaHTe solution under anaerobic environment, and bubbling continuously to make the generated H 2 Te is transferred to the cadmium chloride solution to generate the precursor of CdTe quantum dots. Heat to 105°C and reflux for 30 minutes, remove the heat source, and cool to room temperature. The prepared cadmium telluride quantum dot aque...

Embodiment 2

[0049] 1. Preparation of cadmium telluride quantum dot solution

[0050] The preparation process of this step is the same as in Example 1.

[0051] 2. Preparation of Europium ion solution

[0052] The preparation process of this step is the same as in Example 1.

[0053] 3. Preparation of tetracycline ultrapure water test solution

[0054] The preparation process of this step is the same as in Example 1.

[0055] 4. Preparation of dual emission ratio fluorescent probe

[0056] Add 200 μL of cadmium telluride quantum dot solution, 32 μL of 10 mM europium nitrate solution, 200 μL of 100 mM Tris-HCl buffer solution at pH 8, tetracycline solution and ultrapure water to the cuvette, and the final total volume is 2mL mixed test system (the concentration of tetracycline in this test system varies from 0 to 80 μM), mix well and then stand and incubate for 5 min to make it fully react.

[0057] 5. Drawing of calibration curve for quantitative test

[0058] Use excitation light with a wavelength of ...

Embodiment 3

[0062] 1. Preparation of cadmium telluride quantum dot solution

[0063] The preparation process of this step is the same as in Example 1.

[0064] 2. Preparation of Europium ion solution

[0065] The preparation process of this step is the same as in Example 1.

[0066] 3. Preparation of interference substance to be tested

[0067] Preferably, the inorganic ions (K + , Ca 2+ , Na + , Mg 2+ , Fe 2+ ,Zn 2+ , SO 4 2- , Cl - , CO 3 2- ), amino acids (Gly, His, Lys, Arg, Glu, Cys), antibiotics (chlortetracycline, oxytetracycline, doxycycline, amoxicillin, carbenicillin sodium, gentamicin, kanamycin , Ciprofloxacin, chloramphenicol, sparfloxacin), glutathione, ascorbic acid, glucose and other interference substances to be tested, the concentration is 1M.

[0068] 4. Preparation of dual emission ratio fluorescent probe

[0069] Add 200 μL of cadmium telluride quantum dot solution, 32 μL of 10 mM europium nitrate solution, 200 μL of 100 mM Tris-HCl buffer solution at pH 8, and ultrapure water t...

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Abstract

The invention discloses a high-sensitivity-ratio fluorescent probe based on a composite system of cadmium telluride quantum dots and europium ions and a preparation method and application of high-sensitivity-ratio fluorescent probe. The high-sensitivity-ratio fluorescent probe is the composite system chelated by carboxyl functionalized cadmium telluride quantum dots coated by thiohydracrylic acidand europium ions. The high-sensitivity-ratio fluorescent probe and the preparation method and application thereof have the advantages that the quantum dots and the europium ions which can excited bya single-wavelength light source to emit light are utilized to design and develop a double-emission fluorescent sensor, tetracycline antibiotics in water can be fast detected in real time, the sensoris simple in raw materials, and the preparation method is easy to popularize; the high-sensitivity-ratio fluorescent chemical sensor is high in sensitivity, simple to operate, good in selectivity andcapable of achieving visual detection and quantitative detection.

Description

Technical field [0001] The invention relates to a ratio fluorescent probe for detecting the residues of tetracycline antibiotics, in particular to a high sensitivity ratio fluorescent probe based on a composite system of cadmium telluride quantum dots and europium ions, and a preparation method and application thereof. The field of chemistry and nanomaterials science. Background technique [0002] Tetracycline antibiotics are a kind of broad-spectrum antibiotics with good oral absorption, low price and low toxicity. In recent years, they have been widely used in animal husbandry and fishery to promote the rapid growth and weight gain of animals in the form of pharmaceutical additives. However, the abuse of tetracycline often results in excessive residues in animals and flows into humans along the food chain, resulting in the production of drug-resistant bacteria, and causing adverse consequences such as allergic reactions, gastrointestinal disorders and liver toxicity. At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C01B19/00B82Y30/00
Inventor 蒋长龙杨亮王振洋张淑东刘变化李年刘翠
Owner 中科合肥智慧农业谷有限责任公司
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