Fluorescent probe, enhanced ratio fluorescence sensor, method and application

A fluorescent probe and ratiometric fluorescence technology, which is applied in the field of fluorescence detection, can solve the problems of no fluorescence fluorescence performance, achieve accuracy and anti-interference ability, facilitate visual detection, and eliminate system errors.

Active Publication Date: 2021-09-24
NORTHWEST A & F UNIV
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The zeolite imidazolate framework ZIF-8 is a common metal-organic framework. Due to its porosity, large surface area, and abundant active sites, it has been widely used in the fields of adsorption, photocatalysis, and chemical sensing. However, ZIF-8 itself has no fluorescence or Poor fluorescence performance, mainly used as a carrier for fluorescent probes in sensors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent probe, enhanced ratio fluorescence sensor, method and application
  • Fluorescent probe, enhanced ratio fluorescence sensor, method and application
  • Fluorescent probe, enhanced ratio fluorescence sensor, method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Following the above-mentioned technical scheme, the present embodiment provides a kind of fluorescent probe, which is used to detect tetracycline antibiotics. Prepared by reaction in aqueous solution of hydrate;

[0049] The prepared fluorescent probe has a red fluorescent emission peak at a wavelength of 604nm, and produces a green fluorescent emission peak at a wavelength of 512nm for tetracycline antibiotics, and the particle size of the fluorescent probe is 73-83nm. Fluorescent probes interact with tetracycline antibiotics to generate new emission peaks.

[0050] It specifically comprises the following steps: respectively dissolving zinc nitrate and 2-methylimidazole in methanol, then mixing them, stirring continuously for 1 hour, washing the precipitate with methanol for 3 times, and then performing vacuum freeze-drying to obtain ZIF-8, wherein The molar ratio of zinc ion and 2-methylimidazole is 1:70; the Ru(bpy) 3 2+ Dissolve in ultrapure water, and adjust the...

Embodiment 2

[0061] This embodiment provides an enhanced ratiometric fluorescence sensor and its preparation method. The enhanced ratiometric fluorescence sensor includes the fluorescent probe described in Example 1, comprising the following steps:

[0062] Step 1: The methanol solution dissolved in zinc nitrate and the methanol solution dissolved in 2-methylimidazole are mixed and stirred for reaction to obtain a precipitate, which is the zeolite imidazolate skeleton ZIF-8;

[0063] Step 2: dissolving terpyridine ruthenium chloride hexahydrate in water, and adjusting the pH of the resulting solution with sodium hydroxide; adjusting the pH is to facilitate complexing with ZIF-8;

[0064] The concentration of the solution obtained by dissolving terpyridine ruthenium chloride hexahydrate in water is (3-6) mg / mL, the initial pH of the solution is 8-8.3, and the pH of the solution is adjusted to 12 by using sodium hydroxide.

[0065] Step 3: Precipitate the zeolite imidazolate skeleton ZIF-8 o...

Embodiment 3

[0071] This example verifies the feasibility and specificity of the enhanced ratiometric fluorescent sensor prepared in Example 2 for detecting tetracycline antibiotics.

[0072] Taking tetracycline (TET) as the representative of tetracycline antibiotics, the feasibility test of enhanced ratiometric fluorescent sensor for detection of tetracycline antibiotics is carried out. The specific experimental operations are as follows:

[0073] Put 100 μL ZIF-8 solution in a 2 mL centrifuge tube, add 1 μL tetracycline standard solution with a concentration of 10 mM, dilute to 1 mL with ultrapure water, mix and react for 3 minutes, as control group 1;

[0074] Put 100 μL of the Ru@ZIF-8 solution in Example 2 into a 2 mL centrifuge tube, and dilute it to 1 mL with ultrapure water, as control group 2;

[0075] Put 100 μL of the Ru@ZIF-8 solution in Example 2 into a 2 mL centrifuge tube, add 1 μL of tetracycline standard solution with a concentration of 10 mM, dilute to 1 mL with ultrapure...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a fluorescent probe, an enhanced ratio fluorescence sensor, a method and application. The fluorescent probe is used for detecting tetracycline antibiotics, and is prepared by dispersing a zeolite imidazate skeleton ZIF-8 into an aqueous solution of terpyridyl ruthenium chloride hexahydrate and reacting, wherein the fluorescent probe has an emission peak of red fluorescence at the wavelength of 604 nm, the fluorescent probe generates an emission peak of green fluorescence at the wavelength of 512 nm for the tetracycline antibiotics, and the particle size of the fluorescent probe is 73-83 nm. Herein, the enhanced ratio fluorescence sensor is used for detecting tetracycline antibiotics, has very important significance and application value for monitoring tetracycline antibiotic residues in drinking water and food, so that the fluorescent probe has the advantages of sensitivity, accuracy, rapidness, simplicity in operation and convenience in visualization.

Description

technical field [0001] The invention belongs to the field of fluorescence detection, and in particular relates to a fluorescent probe, an enhanced ratiometric fluorescent sensor, a method and an application. Background technique [0002] Tetracycline antibiotics (TCs) are a class of broad-spectrum antibiotics, mainly including tetracycline (TET), chlortetracycline (CTE), oxytetracycline (OXY) and doxycycline (DOX). Due to its broad-spectrum antibacterial effect, easy availability and low cost, it has been widely used in the prevention and control of microbial infections. However, excessive use or long-term low-dose use will lead to tetracycline residues in animal-derived foods, which in turn will cause Human direct poisoning, liver damage, allergic reaction, etc., will also lead to environmental pollution, and the generation of drug-resistant bacteria. Therefore, it is necessary to monitor the residues of tetracyclines in food. [0003] Traditional detection methods includ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06G01N21/64B82Y20/00B82Y30/00
CPCC09K11/025C09K11/02C09K11/06G01N21/6428G01N21/643B82Y20/00B82Y30/00C09K2211/185Y02A50/30
Inventor 王丽杨铠溶贾珮侯晋杰
Owner NORTHWEST A & F UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap