10 results about "Hemeprotein" patented technology

To provide a food composition that does not contain animal-derived substances and that can be used as a food additive or flavoring to impart spice, umami, or meat seasoning to a food composition by utilizing fungi, yeast, and extracts of Ogataea pholymorpha. SOLUTION: The Ogataea polymorphic fungus yeast, extract, and biomass extract of the Ogataea polymorphic fungus of the present invention contain 0.03 to 3.1% w / w heme. They also contain 0.1 to 25% w / w amino acids. They also contain 0.1 to 2% w / w histidine. They are dissolved or formed by hydrolysis. The Ogataea polymorphic fungus of the present invention further contains proteins, amino acids, carbohydrates, and vitamins. They also contain glutathione. The present invention also relates to the production of recombinant heme proteins in methylotrophic yeast such as Ogataea polymorphic fungus. The Ogataea polymorphic fungus extract contains 0.007% or more 5' ribonucleotides. The 5' ribonucleotides are extracted from 5' GMP.

This invention discloses a heme protein mutant and its application in the preparation of 5-hydroxy-L-tryptophan, belonging to the fields of enzyme engineering and biocatalysis. This invention uses heme protein A2 as the starting protein and provides a method for direct catalysis with the participation of hydrogen peroxide. L A mutant of heme protein with β-tryptophan hydroxylation. The mutant constructed in this invention can be used in a solution containing 3 mmol / L... L In a reaction system containing 5-hydroxy-L-tryptophan, 15 mmol / L hydrogen peroxide, and 20 μmol / L purification enzyme, a 5-hydroxy-L-tryptophan conversion rate of 24.95% was achieved, compared to the single-mutant A2. M1 The percentage of 5-hydroxy-L-tryptophan was increased by approximately 8.63 times from 2.89%; and the area ratio of 5-hydroxy-L-tryptophan in the main monohydroxylation product peak was increased from approximately 49.8% to 97.1%, while the non-target product peak was reduced.

A method to make Co- or Ni-substituted protoporphyrin IX by incubating an E. coli strain in a rich medium containing iron ions and added Co2+ or Ni2+ ions, for a time and under conditions wherein the E. coli strain produces Co- or Ni-substituted protoporphyrin IX. The E. coli host may be transformed to contain and express a gene construct encoding a mutant EcHemH including a L13R mutation.

A cell-free method for synthesizing a hemoprotein or peptide chain. The method comprises: adding a DNA or RNA template encoding a heme-binding protein or peptide chain to a cell-free in-vitro synthesis system so as to synthesize the heme-free form of the heme-binding protein in vitro; and incubating the heme with a binding system containing the heme-binding protein or peptide chain for a predetermined period of time so as to produce a hemoprotein or peptide chain bound to the heme. The method does not require modifications to a strain and fermentation broth, involves simple production, and has universal applicability.

The invention provides a method for cell-free synthesis of heme protein or peptide chain, which is characterized by comprising the following steps: adding a DNA (Deoxyribose Nucleic Acid) or RNA (Ribonucleic Acid) template for coding the heme binding protein or peptide chain into a cell-free in-vitro synthesis system for in-vitro synthesis of heme-free heme binding protein; the heme is incubated with a binding system containing the heme binding protein or peptide chain for a predetermined time to produce a heme protein or peptide chain to which the heme is bound. According to the present invention, the expression of the heme protein can be completed only by providing any DNA or RNA template of the target protein of which the code can be combined with the heme, adding the DNA or RNA template to the cell-free system, and incubating with the heme, the change of the bacterial strain and the fermentation broth is not required, the production is simple, and the method has general applicability.

An object of the present invention is to provide a novel preservative solution for a heme protein and a method for stabilizing a heme protein, which are effective against denaturation or degradation of a heme protein, and the present invention specifically relates to a preservative solution for a heme protein comprising a disulfonic acid or a salt thereof, and a method for stabilizing a heme protein, which involves bringing a disulfonic acid or a salt thereof into coexistence in a sample comprising a heme protein.

A novel meat-like plant protein composition that can be used as fake meat (meat substitute) is developed. Provided is a plant protein composition, wherein the plant protein composition is prepared by hydrolyzing a first plant protein to prepare a peptide, crosslinking the peptide to obtain a crosslinked peptide, and mixing the crosslinked peptide with a heme protein, sugar, and a second plant protein. In addition, it is preferable that the plant protein composition is prepared by mixing and heating the crosslinked peptide and sugar in advance, and then mixing the resulting mixture with a second plant protein. Further, it is preferable that a protein hydrolysate is further mixed in the plant protein composition.

The present disclosure relates to genetically modified bacteria, wherein said genetically modified bacteria is designed to express or overexpress a heme-containing protein. Said protein is optionally expressed or overexpressed in combination with one or more enzymes that catalyze the heme synthesis pathway. Said genetically modified organism allows scalable production of heme-containing protein with the advantage that the expressed protein remains in soluble form without forming inclusion bodies.

The application artificially designs and creates a functional module SyKerMb with high efficient degradation of poultry feather waste and synthesis of new heme protein by using synthetic biology method. The application constructs a recombinant vector of the functional module, and assembles and expresses the same in a chassis microbial cell Escherichia coli. Experiments prove that the engineered strain integrated with the conversion functional module has the biological synthesis capacity of degrading animal waste feather and efficiently synthesizing artificial heme protein, and can be used in artificial protein preparation and future artificial food and other related industrial production.

The present disclosure provides methods of producing heme proteins in transgenic plants, plant tissues, or plant cells, as well as describing the expression of these heme proteins in seeds. Also, the present disclosure provides transgenic plants expressing the heme proteins, myoglobin and hemoglobin, by introducing and integrating the recombinant DNA constructs into the host genetic material of the subject plants. The specific combination of regulatory elements disclosed herein allows for high heme protein expression level in seeds.