4 results about "Hemeprotein" patented technology

This invention discloses a heme protein mutant and its application in the preparation of 5-hydroxy-L-tryptophan, belonging to the fields of enzyme engineering and biocatalysis. This invention uses heme protein A2 as the starting protein and provides a method for direct catalysis with the participation of hydrogen peroxide. L A mutant of heme protein with β-tryptophan hydroxylation. The mutant constructed in this invention can be used in a solution containing 3 mmol / L... L In a reaction system containing 5-hydroxy-L-tryptophan, 15 mmol / L hydrogen peroxide, and 20 μmol / L purification enzyme, a 5-hydroxy-L-tryptophan conversion rate of 24.95% was achieved, compared to the single-mutant A2. M1 The percentage of 5-hydroxy-L-tryptophan was increased by approximately 8.63 times from 2.89%; and the area ratio of 5-hydroxy-L-tryptophan in the main monohydroxylation product peak was increased from approximately 49.8% to 97.1%, while the non-target product peak was reduced.

A novel meat-like plant protein composition that can be used as fake meat (meat substitute) is developed. Provided is a plant protein composition, wherein the plant protein composition is prepared by hydrolyzing a first plant protein to prepare a peptide, crosslinking the peptide to obtain a crosslinked peptide, and mixing the crosslinked peptide with a heme protein, sugar, and a second plant protein. In addition, it is preferable that the plant protein composition is prepared by mixing and heating the crosslinked peptide and sugar in advance, and then mixing the resulting mixture with a second plant protein. Further, it is preferable that a protein hydrolysate is further mixed in the plant protein composition.

The present disclosure relates to genetically modified bacteria, wherein said genetically modified bacteria is designed to express or overexpress a heme-containing protein. Said protein is optionally expressed or overexpressed in combination with one or more enzymes that catalyze the heme synthesis pathway. Said genetically modified organism allows scalable production of heme-containing protein with the advantage that the expressed protein remains in soluble form without forming inclusion bodies.

The present disclosure provides methods of producing heme proteins in transgenic plants, plant tissues, or plant cells, as well as describing the expression of these heme proteins in seeds. Also, the present disclosure provides transgenic plants expressing the heme proteins, myoglobin and hemoglobin, by introducing and integrating the recombinant DNA constructs into the host genetic material of the subject plants. The specific combination of regulatory elements disclosed herein allows for high heme protein expression level in seeds.