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Cyanideless hemolysin and its use

A technology of hemolysin and cyanide, applied in the field of hemolysin composition, can solve problems such as environmental pollution

Inactive Publication Date: 2004-04-21
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still uses certain toxic or carcinogenic compounds, which still has certain pollution to the environment.

Method used

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  • Cyanideless hemolysin and its use
  • Cyanideless hemolysin and its use
  • Cyanideless hemolysin and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Prepare a solution in the following proportions:

[0077] 1. Sodium lauryl sulfate 5g

[0078] 2. Dodecyltrimethylammonium bromide 25g

[0079] 3. Polyethylene glycol octylphenyl ether 5ml

[0080] 4. Distilled water 11

[0081] Use a G4 sand filter to circulate and filter, and use a suitable hematology analyzer or other particle analysis instruments to monitor the blank count value to meet the blank count requirements.

[0082] Pipette 13ul whole blood and add it to 3.5ml diluent, stir evenly, add 0.5ml hemolysin, and measure it on a BC series hematology analyzer.

[0083] figure 1 It is the ultraviolet-visible absorption spectrum that is scanned immediately after the diluted blood is added with hemolysin. There is an absorption peak at 540nm.

[0084] figure 2 It is the white blood cell histogram obtained by the hemolysin of the present invention on the BC-3000+ hematology analyzer produced by Mindray Company.

[0085] image 3 It is the ultraviolet-visible ...

Embodiment 2

[0087] Prepare a solution in the following proportions:

[0088] 1. Cetyltrimethylammonium chloride 10g

[0089] 2. Sodium lauryl sulfate 1.5g

[0090] 3. Emulsifier OP 0.58g

[0091] 4. Double distilled water 11

[0092] Use a G4 sand filter to circulate and filter, and use a suitable hematology analyzer or other particle analysis instruments to monitor the blank count value to meet the blank count requirements.

[0093] Dilute the blood sample with phosphate buffered saline (PBS) as a diluent, add the hemolysin of the above formula, and measure the absorption ultraviolet-visible absorption spectrum as follows: image 3 As shown, at the same time, the hematology analyzer is used for analysis in the pre-dilution mode, and the obtained histogram is as follows Figure 4 shown.

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Abstract

The present invention provides a reliable method to determine lysin of ferrohemoglobin, and meanwhile white cell can be divided into two subgroups for counted separately. How to use the lisin is also included in the present invention. The lysin with no cyanide includes at least one type of surfactant group dissolve red cell sufficiently and to release magnitude of magnitude ferrohemoglobin, at least one type of positive ion surfactant, at least one type of negative ion surfactant and at least one type of non-ion surfactant.

Description

technical field [0001] The present invention relates to a hemolysin composition for measuring the total hemoglobin concentration in a blood sample (manually or automatically) and for simultaneously performing white blood cell counting or differential counting of white blood cell subgroups and a method of use. Background technique [0002] The determination of total hemoglobin is an important indicator of blood oxygen carrying capacity. So far, more than 300 kinds of hemoglobin have been found in normal people and patients with blood diseases. These abnormalities lead to changes in hemoglobin concentration or changes in the oxygen-carrying capacity of hemoglobin, resulting in diseases such as sickle cell anemia, thalassemia and hemoglobin abnormalities. [0003] Determining the concentration of hemoglobin in blood samples is a very important basis for the diagnosis of related diseases, and it is also very important for monitoring the treatment of hemoglobin diseases and other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/72
Inventor 徐祖越
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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