Determination method of balsum pear saponin
A kind of balsam pear saponin and determination method technology, applied in the field of determination of balsam pear saponin, can solve the problems of difficulty in determination of balsam pear saponin, low purity of balsam pear saponin, inaccurate method for content of balsam pear saponin and the like
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Embodiment 1
[0029] A method for assaying bitter melon saponins, comprising the following steps:
[0030] (1) Preparation of Momordica saponin reference substance
[0031] ① Extraction of Crude Momordica Saponins
[0032] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:30 by weight, then add cellulase at 4 mg / ml, act at 45°C for 2 hours, then treat with 750W microwave for 45 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.06Mpa, and concentrate under reduced pressure to the original 1 / 7 of the volume to obtain the crude extract of Momordica saponin; add 1 times the volume of ether to the crude extract of Momordica saponin, extract twice to remove fat-soluble impurities, and take the lower aqueous phase; then use 0.5 times the vol...
Embodiment 2
[0052] A method for assaying bitter melon saponins, comprising the following steps:
[0053] (1) Preparation of Momordica saponin reference substance
[0054] ① Extraction of Crude Momordica Saponins
[0055] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:15 by weight, then add cellulase at 6 mg / ml, act at 60°C for 1 hour, then treat with 5000W microwave for 10 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.05Mpa, and concentrate under reduced pressure to the original 1 / 5 of the volume to obtain the crude extract of Momordica saponin; add 0.5 times the volume of petroleum ether to the crude extract of Momordica saponin, extract once, remove fat-soluble impurities, and take the lower aqueous phase; then use an equal...
Embodiment 3
[0070] A method for assaying bitter melon saponins, comprising the following steps:
[0071] (1) Preparation of Momordica saponin reference substance
[0072] ① Extraction of Crude Momordica Saponins
[0073] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:40 by weight, then add cellulase at 2 mg / ml, act at 30°C for 3 hours, then treat with 500W microwave for 60 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.07Mpa, and concentrate under reduced pressure to the original 1 / 10 of the volume to obtain the crude extract of Momordica saponin; add 2 times the volume of petroleum ether to the crude extract of Momordica saponin, extract 3 times, remove fat-soluble impurities, and take the lower aqueous phase; then use 2 time...
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