Determination method of balsum pear saponin

A kind of balsam pear saponin and determination method technology, applied in the field of determination of balsam pear saponin, can solve the problems of difficulty in determination of balsam pear saponin, low purity of balsam pear saponin, inaccurate method for content of balsam pear saponin and the like

Inactive Publication Date: 2006-01-25
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the above method, the method of using ginsenoside as a reference substance to measure the content of balsam pear saponins in bitter melon is not accurate. precise
Using the β-sitosterol standard as a control, the method of calculating the content of bitter melon saponins is even more inaccurate, because from the existing research, there are many types of bitter melon saponins in bitter melon, not just β-sitosterol-3-glucose A mixture of glucoside and 5,25-stigmastadienol-3-glucoside at a ratio of 1:1
The best results are obtained with Momordica saponin as the reference substance, but there is no report on the purification and preparation of the reference substance of Momordica saponin, and the purity of the available Momordica saponin is too low to be used as the reference substance, so the determination of Momordica saponin is more difficult

Method used

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  • Determination method of balsum pear saponin

Examples

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Embodiment 1

[0029] A method for assaying bitter melon saponins, comprising the following steps:

[0030] (1) Preparation of Momordica saponin reference substance

[0031] ① Extraction of Crude Momordica Saponins

[0032] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:30 by weight, then add cellulase at 4 mg / ml, act at 45°C for 2 hours, then treat with 750W microwave for 45 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.06Mpa, and concentrate under reduced pressure to the original 1 / 7 of the volume to obtain the crude extract of Momordica saponin; add 1 times the volume of ether to the crude extract of Momordica saponin, extract twice to remove fat-soluble impurities, and take the lower aqueous phase; then use 0.5 times the vol...

Embodiment 2

[0052] A method for assaying bitter melon saponins, comprising the following steps:

[0053] (1) Preparation of Momordica saponin reference substance

[0054] ① Extraction of Crude Momordica Saponins

[0055] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:15 by weight, then add cellulase at 6 mg / ml, act at 60°C for 1 hour, then treat with 5000W microwave for 10 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.05Mpa, and concentrate under reduced pressure to the original 1 / 5 of the volume to obtain the crude extract of Momordica saponin; add 0.5 times the volume of petroleum ether to the crude extract of Momordica saponin, extract once, remove fat-soluble impurities, and take the lower aqueous phase; then use an equal...

Embodiment 3

[0070] A method for assaying bitter melon saponins, comprising the following steps:

[0071] (1) Preparation of Momordica saponin reference substance

[0072] ① Extraction of Crude Momordica Saponins

[0073] Add distilled water to the bitter melon seed powder dried and crushed through a 60-mesh sieve at a ratio of 1:40 by weight, then add cellulase at 2 mg / ml, act at 30°C for 3 hours, then treat with 500W microwave for 60 minutes, and filter. Centrifuge, collect the supernatant, pass through a 0.2 μm hollow fiber membrane, take the permeate, pass through a hollow fiber membrane with a molecular weight cut-off of 6000, take the permeate in a rotary evaporator, 60°C, 0.07Mpa, and concentrate under reduced pressure to the original 1 / 10 of the volume to obtain the crude extract of Momordica saponin; add 2 times the volume of petroleum ether to the crude extract of Momordica saponin, extract 3 times, remove fat-soluble impurities, and take the lower aqueous phase; then use 2 time...

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Abstract

The invention discloses a balsam pear saponin determining method, including the following steps: 1. preparing a balsam pear saponin control sample: (1) extracting coarse product of balsam pear saponin, i.e. adding cellulose enzyme into balsam pear seed powder for microwave processing, and filtering and collecting supernatant liquor, passing the supernatant liquor through hollow fiber film, taking penetrating fluid to pass it through the other hollow fiber film for holding back molecular weight-6000 substances, taking penetrating fluid again for concentrating, and obtaining balsam pear saponin coarse extractant for purifying and drying, so as to obtain the coarse product; (2) pre-purifying for preparing front balsam pear saponin by HPCL separation; (3) preparing by HPLC separation; (4) re-crystallizing the product; 2. determining the saponin content of the sample: adopting light-splitting photometric method to measure light absorptivity in 468 nm wavelength, calculating to obtain the content. The determining method provides standard control sample, accurate and reliable.

Description

technical field [0001] The invention relates to a method for determining compounds, in particular to a method for determining bitter melon saponins. Background technique [0002] Bitter melon saponin is one of the main active components of bitter gourd. There have been a lot of reports on the determination of bitter gourd saponin in bitter gourd and its products. At present, the determination of Momordica saponins at home and abroad mainly uses ginsenosides or Momordica saponins as reference substances, and is determined by spectrophotometry. [0003] Chen Xun et al. (Chen Xun, Yu Haining, Tang Desong, etc., Research on Rapid Separation and Purification of Momordica saponins, Food Science, 2004, 25 (2): 114-117) extracted bitter melon with ethanol, and used ginsenoside Rg1 as a reference substance. Measure the absorbance at 548nm to determine the total amount of bitter melon saponins. Sun Lei (Sun Lei, the technology research of extracting bitter melon saponin with ethanol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/34B01D15/08
Inventor 刘金福张平平连喜军臧凤艳
Owner TIANJIN AGRICULTURE COLLEGE
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