Personal care composition containing leghemoglobin

a technology of leghemoglobin and composition, which is applied in the field of personal care compositions, can solve the problems of lanolin, a by-product derived from sheep wool shearing, suffering, and more likely to have a detrimental effect on human health, and bacteria, however, will not convert nitrogen into ammonia when used

Inactive Publication Date: 2011-11-24
GRUBER JAMES V
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity of the personal care industry to these issues is so great that even the sale of lanolin, a by-product derived from the shearing of sheep wool, has suffered because it is considered to be an animal-derived product.
Human-derived products can have even greater concerns than animal-derived constituents because pathogens borne in the human-derived cosmetic ingredients are even more likely to have a detrimental effect on human health.
Fortunately, while plants and animals are incapable of chemically converting nitrogen into useful nitrogen-containing components, certain bacteria, especially those of the blue-green Cyanobacteria Kingdom, such as, for example, Rhizobium, have evolved a mechanism to convert nitrogen into more useful ammonia.
Interestingly, Rhizobium bacteria, however, will not convert nitrogen into ammonia when they exist alone.
The bacteria are generally unable to keep the oxygen away that is detrimental to the overall nitrogen fixation reaction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0036]Two kilograms of freshly pulled soybean (Glycine max) plants including the roots were washed with a gentle stream of deionized water to remove residual soil. The nitrogen fixation nodules, which were readily apparent on well-infected roots, were easily removed by hand and were collected. To approximately 100 grams of isolated nodules was added 200 mls of deionized water and the entire mixture was macerated in a Waring blender at high shear. The resulting dark heterogeneous lysate was filtered through cheesecloth to remove large pieces of undissolved material. The resulting hazy, dark solution was further purified by filtration through fine filter paper and by carbon and sterile filtration that removed the organisms responsible for a strong earthy odor in the extract.

[0037]The level of leghemoglobin in this particular extract was found to be approximately 0.3 mg / ml as determined by the following analysis method. To a 10 ml sample of the extract was added 1 ml of 0.1M sodium dit...

example 2

[0038]Two kilograms of roots containing active nitrogen fixation nodules were obtained from Lotus plant (Lotus japonicus) grown hydroponically in vermiculite inoculated with bacteria from the Genus Brachyorhizobium. Approximately 100 grams of the nitrogen fixation nodules were cleaned and removed and the resulting nodules were extracted by macerating the nodules in a Waring blender with 200 mls of 80% aqueous ethanol. The resulting heterogeneous mixture was filtered as described above. The ethanol was removed via low tempertuare vacuum evaporation and replaced with a comparable amount of deionized water providing a root nodule extract containing approximately 0.6 mg / ml of leghemoglobin as analyzed using the method described in Example 1.

example 3

[0039]Samples of root nodules obtained from Alfalfa (Medicago sativa) were treated in a similar fashion as described above in Example 1. HPLC analysis of these extracts for the presence of the cytokinins zeatin (a pure sample of zeatin was obtained from Serva (Islandia, N.Y.) provided concentrations comparable to those reported in the literature, in particular, Hashizume, T. et al., Agric. Biol. Chem 1985; 49: 3481-3484. The extract was analyzed using the method described in Example 1 and was found to comprise approximately 0.5 mg / ml of leghemoglobin.

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Abstract

A personal care composition comprising leghemoglobin and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. Preferably, the leghemoglobin is a nitrogen fixation root nodule extract providing a leghemoglobin concentration in the composition of between 0.0001% and about 10% based upon the total weight of the composition. Also disclosed is a method for preparing the personal care composition.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This is a divisional application of U.S. application Ser. No. 10 / 366,231, filed Feb. 13, 2003, which claims the benefit of U.S. Provisional Application No. 60 / 357,544, filed Feb. 15, 2002, the disclosures of which are incorporated by reference herein in their entirety.FIELD OF THE INVENTION[0002]This invention relates generally to personal care compositions, and more specifically to such compositions containing leghemoglobin. The leghemoglobin is suitably extracted from nitrogen fixation root nodules. The leghemoglobin provides advantageous properties to the personal care composition during use by facilitating the control of reactive oxygen and nitric oxide free radicals on the skin.BACKGROUND OF THE INVENTION[0003]Personal care compositions encompass a wide variety of applications, including soaps, shampoos, skin care medicaments, cosmetics, as well as therapeutic and homeopathic skin care formulations. The use of plant-derived raw mater...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K8/64A61K8/66A61Q19/00A61K8/02A61K8/00A61K8/06A61K8/33A61K8/49A61K8/96A61K8/97A61Q1/00A61Q1/04A61Q1/06A61Q17/00A61Q19/04
CPCA61K8/068A61K8/645A61Q19/04A61Q17/00A61Q19/00A61K8/97A61K8/9767A61K8/9728A61K8/9789A61P43/00A61P7/00A61P7/02A61K38/16A61K36/00
Inventor GRUBER, JAMES V.
Owner GRUBER JAMES V
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