Electrochemical biosensor electrode for detection of hydrogen peroxide and preparation method thereof

A biosensor, hydrogen peroxide technology, applied in scientific instruments, material analysis by electromagnetic means, instruments, etc., to achieve the effect of rapid detection, simple method, and accelerated electron transfer

Inactive Publication Date: 2013-07-17
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of helical carbon nanofiber...

Method used

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  • Electrochemical biosensor electrode for detection of hydrogen peroxide and preparation method thereof
  • Electrochemical biosensor electrode for detection of hydrogen peroxide and preparation method thereof
  • Electrochemical biosensor electrode for detection of hydrogen peroxide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Preparation of Enzyme Biosensor

[0028] Weigh 51mg of NiO powder and put it into a porcelain cup, put it into a quartz glass tube, then react for 1 hour at 375°C in an atmosphere of hydrogen, and finally react for 0.5 hour in an acetylene atmosphere at 415°C to obtain 0.364g of helix Carbon nanofibers, the product can be used after cooling to room temperature.

[0029] Weigh 20 mg of helical carbon nanofibers and disperse them into 10 mL of 0.25% by mass PDDA (containing 0.5 M NaCl) aqueous solution, obtain a uniform black suspension after ultrasonication for 10 min, and wash with distilled water until the pH is neutral . Next, take 1mL of the helical carbon nanofiber solution obtained above, disperse 10mL of gold colloid with a particle size of about 10-30nm in it, and sonicate, and then add 1mL of gold colloid in sequence, and sonicate for 10min each time gold colloid is added, until A total of 15 mL of gold colloid was added, and finally, the supernatan...

Embodiment 2

[0032] Example 2: Preparation of Enzyme Biosensor

[0033] In Example 1, "20 mg of helical carbon nanofibers are dispersed into 10 mL of PDDA with a concentration of 0.25% by mass" is changed to "20-30 mg of helical carbon nanofibers are dispersed into 5-30 mL of PDDA with a concentration of 0.25% by mass", and prepared The other conditions are the same as in Example 1, and the performance of the obtained sensor is similar to Example 1, and can be used for the quantitative detection of hydrogen peroxide.

Embodiment 3

[0034] Example 3: Preparation of Enzyme Biosensor

[0035] In Example 1, "PDDA with a concentration of 0.25% by mass" was changed to PDDA with a concentration of 0.05 to 0.5% by mass", and the other conditions of preparation were the same as in Example 1, and the performance of the obtained sensor was similar to that of Example 1. One, it can be used for the quantitative detection of hydrogen peroxide.

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Abstract

The invention provides an enzyme biosensor for detection of hydrogen peroxide and a preparation method thereof. The biosensor employs a classic three-electrode system, wherein a reference electrode is a saturated calomel electrode, a counter electrode is a platinum electrode and a working electrode is a glassy carbon electrode. The biosensor provided by the invention promotes electron transfer between hemoglobin and the electrodes mainly by using a composite material of a helical carbon nanofiber and a colloidal gold nanoparticle as a carrier of hemoglobin, so sensitivity of detection of hydrogen peroxide is effectively improved and a satisfactory result is achieved.

Description

technical field [0001] The invention belongs to the field of hydrogen peroxide detection, in particular to a sensor for detecting hydrogen peroxide and a preparation method thereof. technical background [0002] With the continuous promotion of the application of hydrogen peroxide in the fields of electronics, light industry, food, environmental protection, medicine, etc. Cleaning, etc., but at the same time, hydrogen peroxide may also cause various diseases and bring hidden dangers to people's life and health. Therefore, the rapid detection of hydrogen peroxide is particularly important. There are many detection technologies related to hydrogen peroxide, among which, the advantages of using electrochemical method to detect hydrogen peroxide are simple, fast and high sensitivity. [0003] In recent years, enzyme biosensors have attracted the attention of many researchers. Since the catalytic active centers of most enzymes are deeply buried inside the enzyme protein, and th...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 崔荣静韩志达张根华翟牧牧
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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