Fluorescent nanoprobes, and preparation method and applications thereof

Abstract

The invention discloses fluorescent nanospheres, a preparation method thereof, and applications of the fluorescent nanospheres in the field of chemical sensing. The fluorescent nanospheres, which are fluorescent nanoprobes and are uniform in particle size distribution, are obtained via catalytic auto-polymerization of 3-aminophenylboronic acid in an aqueous solution at the presence of a catalyst, and via ultrafiltration processing. The fluorescent nanoprobes can be combined with adenosine monophosphate-modified oxidized grapheme so as to form a fluorescence resonance energy transfer system; when cis-dyhydroxy biological molecules are added into the fluorescence resonance energy transfer system, because of competition on boron affinity interaction, the fluorescent nanospheres and the adenosine monophosphate modified oxidized grapheme are separated, and combination of the fluorescent nanospheres with the cis-dyhydroxy biological molecules is realized, fluorescence of the fluorescent nanospheres is recovered, and in a certain concentration range, fluorescence intensity and the concentration of the cis-dyhydroxy biological molecules are in a linear relationship. Fluorescence properties of the fluorescent nanoprobes are stable, and are not easily influenced by environmental factors. The preparation method is simple; identification selectivity and capacity of resisting disturbance of the fluorescent nanoprobes are excellent.

Description

technical field [0001] The invention relates to the fields of functionalized materials and fluorescent nanometer probes, as well as molecular recognition and chemical sensing. Background technique [0002] Fluorescent probes are a class of biomolecular sensors with the advantages of high sensitivity and fast response time, and are one of the most widely used fluorescence spectroscopy techniques. So far, thousands of fluorescent probes have been reported in the literature. It can be roughly divided into two categories: endogenous fluorescent probes and exogenous fluorescent probes: endogenous fluorescent probes are mainly amino acids, proteins and nucleic acids with fluorescent properties. Such fluorescent probes usually contain Amino acids with aromatic ring structures such as tryptophan, tyrosine, and phenylalanine, or bases with fluorescent properties; exogenous fluorescent probes mainly refer to those with strong fluorescent active groups labeled or derivatized by fluore...

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Problems solved by technology

[0006] In order to overcome the shortcomings of existing fluorescent probes such as cumbersome preparation and poor fluorescence stability, the purpose of the present invention is to provide a simple preparation, good selectivity, stable fluorescence properties, and less susceptible to environmental factors (such as fluorescence quenchers, enhancers and pH, etc. ) Affected fluorescent nanoprobes and their preparation methods and their applications in chemical sensing

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