Fluorescent nanoprobes, and preparation method and applications thereof

A fluorescent nanoprobe and nanosphere technology, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of cumbersome preparation of fluorescent probes, poor fluorescence stability, etc. Simple method, wide linear range effect

Active Publication Date: 2014-07-23
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to overcome the shortcomings of existing fluorescent probes such as cumbersome preparation and poor fluorescence stability, the purpose of the present invention is to provide a simple preparation, good selectivity, stabl

Method used

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  • Fluorescent nanoprobes, and preparation method and applications thereof
  • Fluorescent nanoprobes, and preparation method and applications thereof
  • Fluorescent nanoprobes, and preparation method and applications thereof

Examples

Experimental program
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Example Embodiment

[0028] Example 1: Preparation of poly-m-aminophenylboronic acid nanospheres

[0029] The reaction route is as figure 1 Shown. First, prepare a 0.1M phosphate buffer (pH 10.5) containing 1-10 mg / mL meta-aminophenylboronic acid to obtain a colorless and transparent mixed solution; then add 30% hydrogen peroxide to the mixed solution, in which hydrogen peroxide is added The amount is 1.5 times the volume of the mixed solution, and the reaction is stirred at 25°C to 30°C for about 5 hours to obtain a bright yellow solution. The bright yellow solution was ultrafiltered with an ultrafiltration tube with a molecular weight cut-off of 50,000 Da, and the filtrate was transferred to an ultrafiltration tube with a molecular weight cut-off of 3000 Da to continue ultrafiltration until the volume was less than 200 microliters, and the fraction with a molecular weight of 3000 Da to 50000 Da was collected. After freeze-drying, the poly-m-aminophenyl borate compound nanosphere powder is obtained...

Example Embodiment

[0030] Example 2: Characterization of particle size, ultraviolet absorption and fluorescence properties of poly-m-aminophenylboronic acid nanospheres

[0031] (1) Dynamic light scattering characterizes particle size distribution

[0032] The poly-m-aminophenylboronic acid nanospheres prepared in Example 1 were dissolved in ultrapure water to prepare a solution with a concentration of 5 mg / mL. After 1 hour of ultrasound, the particle size distribution was characterized by a dynamic light scattering instrument. The results are as follows image 3 A. It can be seen from the figure that the prepared poly-m-aminophenylboronic acid nanospheres have a uniform particle size distribution, with a particle size of about 5-10 nm. The result is consistent with the TEM characterization.

[0033] (2) Ultraviolet absorption spectrum and fluorescence spectrum

[0034] The poly-m-aminophenylboronic acid nanospheres prepared in Example 1 were dissolved in ultrapure water to prepare a solution with a con...

Example Embodiment

[0035] Example 3: Investigation on the fluorescence stability of poly-m-aminophenylboronic acid nanospheres and fluorescent substituted boric acid

[0036] Take appropriate amount of m-aminophenylboronic acid, p-vinylphenylboronic acid, pyridine boronic acid, pyrimidine boronic acid and poly-m-aminophenylboronic acid nanospheres and dissolve them in 0.1M phosphate buffer (pH10.5), respectively, to prepare the concentration to 0.25 The mg / mL solution, each solution was divided into 8 parts, and the different concentrations of fructose, glucose, lysozyme, aniline, adenosine, sodium chloride and hydrogen peroxide were investigated respectively; and the influence of pH on the fluorescence intensity of each solution. The result is Figure 4 . It can be seen from the figure that, compared with fluorescent substituted boric acid, poly-m-aminophenylboronic acid nanospheres have good fluorescence stability and are not easily affected by various conditions.

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Abstract

The invention discloses fluorescent nanospheres, a preparation method thereof, and applications of the fluorescent nanospheres in the field of chemical sensing. The fluorescent nanospheres, which are fluorescent nanoprobes and are uniform in particle size distribution, are obtained via catalytic auto-polymerization of 3-aminophenylboronic acid in an aqueous solution at the presence of a catalyst, and via ultrafiltration processing. The fluorescent nanoprobes can be combined with adenosine monophosphate-modified oxidized grapheme so as to form a fluorescence resonance energy transfer system; when cis-dyhydroxy biological molecules are added into the fluorescence resonance energy transfer system, because of competition on boron affinity interaction, the fluorescent nanospheres and the adenosine monophosphate modified oxidized grapheme are separated, and combination of the fluorescent nanospheres with the cis-dyhydroxy biological molecules is realized, fluorescence of the fluorescent nanospheres is recovered, and in a certain concentration range, fluorescence intensity and the concentration of the cis-dyhydroxy biological molecules are in a linear relationship. Fluorescence properties of the fluorescent nanoprobes are stable, and are not easily influenced by environmental factors. The preparation method is simple; identification selectivity and capacity of resisting disturbance of the fluorescent nanoprobes are excellent.

Description

technical field [0001] The invention relates to the fields of functionalized materials and fluorescent nanometer probes, as well as molecular recognition and chemical sensing. Background technique [0002] Fluorescent probes are a class of biomolecular sensors with the advantages of high sensitivity and fast response time, and are one of the most widely used fluorescence spectroscopy techniques. So far, thousands of fluorescent probes have been reported in the literature. It can be roughly divided into two categories: endogenous fluorescent probes and exogenous fluorescent probes: endogenous fluorescent probes are mainly amino acids, proteins and nucleic acids with fluorescent properties. Such fluorescent probes usually contain Amino acids with aromatic ring structures such as tryptophan, tyrosine, and phenylalanine, or bases with fluorescent properties; exogenous fluorescent probes mainly refer to those with strong fluorescent active groups labeled or derivatized by fluore...

Claims

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Application Information

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IPC IPC(8): C09K11/06C08G79/08G01N21/64
Inventor 刘震王双寿
Owner NANJING UNIV
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