Method for high-sensitivity detection of aflatoxin and application of aflatoxin

A technology for sensitive detection of aflatoxins, applied in the field of highly sensitive detection of aflatoxins, can solve problems such as fluorescence instability, and achieve the effects of good fluorescence stability, improved detection sensitivity, and high standard addition recovery.

Active Publication Date: 2016-06-08
云南健牛环境监测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also problems of unstable fluorescence after pre-column derivatization and additional post-column derivatization equipment

Method used

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  • Method for high-sensitivity detection of aflatoxin and application of aflatoxin
  • Method for high-sensitivity detection of aflatoxin and application of aflatoxin
  • Method for high-sensitivity detection of aflatoxin and application of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 utilizes the present invention to detect the content of aflatoxin in peanut, and the steps are:

[0026] 1. Take an appropriate amount of aflatoxin reference substance stock solution, dilute it with acetonitrile to obtain aflatoxin B1 and G1 reference substance solutions with concentrations of 0.5, 10, 50, 100, 150, and 200 ng / mL respectively, and add 20% trifluoroacetic acid And after irradiating for 5min under the ultraviolet light, pass through 0.45m organic filter membrane, C18 chromatographic column (4.6mm * 250mm, 5.0 μ m), mobile phase methanol-water (45: 55; v / v), flow velocity 1.0mL / min, The injection volume was 50 μL, and the column temperature was 30°C. The detector is a fluorescence detector with an excitation wavelength of 360nm and an emission wavelength of 440nm; Agilent1200 high performance liquid chromatography (quaternary pump, autosampler, equipped with a fluorescence detector). With the concentration as the abscissa and the peak area as...

Embodiment 2

[0032] Embodiment 2 Utilizes the present invention to detect the content of aflatoxin in corn, and the steps are:

[0033] Sample determination: Accurately weigh 3g of crushed and homogenized corn sample, accurate to 0.0001g, add 30mL of acetonitrile aqueous solution with a volume fraction of 75%, shake vigorously for 1min, ultrasonically extract in a water bath at 50±2°C for 25min, and centrifuge at 4000r / min for 8min , take out the upper layer solution, repeat the extraction 3 times, and combine the supernatant; the derivation conditions and chromatographic conditions are the same as that of Example 1, step 1, and the sample injection detection is carried out, and the content of aflatoxin B1 is 5.41 μg / kg, and the content of aflatoxin G1 is 5.41 μg / kg. The content is 1.49μg / kg.

Embodiment 3

[0034] Embodiment 3 utilizes the present invention to detect the content of aflatoxin in wolfberry, and the steps are:

[0035] Sample determination: Accurately weigh 5g of crushed and homogenized Lycium barbarum sample, accurate to 0.0001g, add 50mL of acetonitrile aqueous solution with a volume fraction of 80%, shake vigorously for 1min, ultrasonically extract in a water bath at 50±2°C for 25min, and centrifuge at 5000r / min for 5min , take out the upper layer solution, repeat the extraction 2 times, and combine the supernatants; the derivation conditions and chromatographic conditions are the same as those in Example 1, step 1, and the sampling detection is carried out, and the content of aflatoxin B1 is 5.41 μg / kg, and the content of aflatoxin G1 The content is 1.49μg / kg.

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Abstract

The invention discloses a novel method for rapidly detecting aflatoxin in food. The method comprises main steps as follows: an existing precolumn derivatization technology and post-column derivatization technology for aflatoxin are combined, precolumn derivatization is performed under the irradiation of ultraviolet light, a derivative reaction is performed completely, produced fluorescence is strong, the fluorescence stability of a derived product is good, and meanwhile, the detection sensitivity is also improved. Compared with methods for measuring aflatoxin in existing standards, the method has the characteristics that the detection sensitivity is high, post-column light derivatization equipment is not required, the reproducibility is good, the detection is accurate and the like.

Description

technical field [0001] The invention relates to a method for highly sensitive detection of aflatoxin and its application. It belongs to the technical field of detection. Background technique [0002] Aflatoxin (AFT) is a class of secondary metabolites containing a dihydrofuran ring structure produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. It is the most toxic type of mycotoxin found so far. There are more than 20 kinds of aflatoxins, and the more common ones in food include aflatoxin B1, B2, G1, G2 and M1, etc., and the order of their toxicity is AFTB1>AFTM1>AFTG1>AFTB2>AFTG2. The harm of aflatoxins to animals and humans is mainly liver damage, which can cause hepatitis, cirrhosis, liver necrosis, liver cancer and other diseases, and is one of the main factors that induce malignant tumors such as primary hepatocellular carcinoma. Aflatoxins are commonly found in corn, grains, peanuts, and other tree nuts. [0003] During the detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N21/64
CPCG01N21/64G01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 焦扬杨亚玲赵娇
Owner 云南健牛环境监测有限公司
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