Solid-phase PCR kit for gene detection of hypertension medication

A technology for gene detection and hypertension is applied in the field of solid-phase PCR kits for gene detection of hypertension drugs, which can solve the problems of low detection efficiency and inconvenient operation, and achieve the effects of high sensitivity, simple operation and complete detection sites.

Inactive Publication Date: 2017-12-05
银川长缨基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a solid-phase PCR kit for the detection of hypertension drug genes in order to overcome the defects of low detection efficiency and inconvenient operation in the prior art

Method used

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  • Solid-phase PCR kit for gene detection of hypertension medication
  • Solid-phase PCR kit for gene detection of hypertension medication

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The solid-phase PCR kit for drug gene detection for hypertension consists of a PCR reaction mixture and 8-strip tubes or a 96-well PCR reaction plate, in which the PCR reaction mixture is 5×PCR Buffer (Tris-HCl pH8.5 100mM, KCl 500nM , MgCl2 15nM), dN(U)TPs(1:1:1:2), Mg2+, 5 pairs of primers and hot start DNA polymerase.

[0077] The kit is to seal a layer of paraffin wax mixture on the surface of the above PCR reaction mixture, and the paraffin wax mixture is formed by mixing solid paraffin and liquid paraffin at a ratio of 1:8 (W / V).

[0078] The reaction system (25ul) of the kit is: PCR reaction solution 23ul, template 2ul.

Embodiment 2

[0080] Kit operation and result determination

[0081] (1), extraction of genomic DNA

[0082] Use the blood / cell / tissue genome extraction kit (Tiangen Biochemical Technology, product number: DP304) to extract DNA according to the kit instructions;

[0083] (2), preparation of reaction system

[0084] Take 23ul of Hypertension Medication Solid-Phase PCR Reaction Kit 8-strip tube or 96-well PCR reaction plate, add 2ul of extracted DNA;

[0085] (3), PCR amplification

[0086] Put the PCR tube into an ordinary PCR machine for PCR amplification;

[0087] (4), 1.0% agarose gel electrophoresis analysis PCR product;

[0088] (5) Interpretation of results

[0089] The results of this kit are interpreted according to the following criteria

[0090] Table 2

[0091] detection target

Embodiment 3

[0093] Interpretation of sequencing results and medication guidance

[0094] Using the DNA extracted from one case of whole blood as a template, after amplifying with the above kit, the following sequencing results are obtained, and the corresponding medication instructions are given as follows according to the sequencing results

[0095] detection target

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Abstract

The invention discloses a solid-phase PCR kit for gene detection of hypertension medication. The solid-phase PCR kit comprises hot-start DNA polymerase, uracil-N-glycosylase, a PCR reaction buffer solution, dNTPs and a primer. The invention further discloses a detection method adopting the solid-phase PCR kit for gene detection of hypertension medication. The detection method comprises the following specific steps: collecting a sample, extracting nucleic acid, wherein the concentration is not lower than 10ng / ul, and the purity is 1.8-2.0; by taking the nucleic acid sample as a template, carrying out solid-phase PCR amplification reaction by adopting the prepared solid-phase PCR kit for gene detection of hypertension medication, wherein the 25ul PCR reaction system comprises 23ul the solid-phase PCR kit for gene detection of hypertension medication and 2ul the nucleic acid template; carrying out electrophoresis analysis on the PCR product by adopting 1.0% agarose gel; and carrying out result judgment. The solid-phase PCR kit has the advantages that the operation is convenient, the detection efficiency is high, etc.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a solid-phase PCR kit for gene detection of hypertension medication. Background technique [0002] Most of the drugs are metabolized by the liver and then excreted by the kidneys. If the drugs are used improperly, the toxicity will easily accumulate in the body and cause harm to the body. Children are different from adults in that their organ functions are not yet fully developed, and the liver detoxification and kidney excretion functions Weaker, so the tolerance to drugs is poor, it is easy to cause adverse reactions and even cause serious harm to health than the disease itself. [0003] A large number of medical studies have shown that most of the individual differences in drug responses are caused by genetic factors. Since drugs undergo biotransformation processes of absorption, distribution, metabolism and excretion in the body, the entire process is mainly controlled by genes....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q1/686C12Q2600/106C12Q2600/156C12Q2521/101C12Q2521/531
Inventor 韦玉军李航刘文干刘伟顾鹏
Owner 银川长缨基因科技有限公司
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