Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies
A chemiluminescence detection, Treponema pallidum technology, applied in measuring devices, scientific instruments, instruments, etc., can solve problems such as danger and limitation, and achieve the effects of good stability, high sensitivity and high sensitivity
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[0037] The present invention uses gold magnetic particles as a carrier to coat one or more of Treponema pallidum-specific recombinant protein antigens TP15, TP17, TP47, and TP44. for luminescence detection.
[0038] The following specific examples are further descriptions of the present invention, which do not limit the protection scope of the present invention.
[0039] A method for detecting Treponema pallidum antibodies in serum by magnetic enzyme immunochemiluminescence, comprising:
[0040] (1) Coated
[0041] (1.1) Pretreatment: take 100 μL of 5 mg / mL gold magnetic particles, wash them twice with 200 μL of Tris-HCl coupling buffer solution with pH=7.4 and a concentration of 0.02M to balance the pH of the magnetic particles.
[0042] (1.2) Coupling: Dissolve the three Treponema pallidum-specific recombinant protein antigens TP15, TP17, and TP47 in 200 μL of pH=7.4 and a concentration of 0.02M Tris-HCl coupling buffer, mix well and add pretreated gold In the magnetic pa...
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