Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies

A chemiluminescence detection, Treponema pallidum technology, applied in measuring devices, scientific instruments, instruments, etc., can solve problems such as danger and limitation, and achieve the effects of good stability, high sensitivity and high sensitivity

Inactive Publication Date: 2010-05-26
XIAN GOLDMAG NANOBIOTECH
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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for detecting Treponema pallidum antibodies by magnetic enzyme-immunochemiluminescence, which s

Method used

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  • Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies
  • Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies
  • Method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies

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Embodiment Construction

[0037] The present invention uses gold magnetic particles as a carrier to coat one or more of Treponema pallidum-specific recombinant protein antigens TP15, TP17, TP47, and TP44. for luminescence detection.

[0038] The following specific examples are further descriptions of the present invention, which do not limit the protection scope of the present invention.

[0039] A method for detecting Treponema pallidum antibodies in serum by magnetic enzyme immunochemiluminescence, comprising:

[0040] (1) Coated

[0041] (1.1) Pretreatment: take 100 μL of 5 mg / mL gold magnetic particles, wash them twice with 200 μL of Tris-HCl coupling buffer solution with pH=7.4 and a concentration of 0.02M to balance the pH of the magnetic particles.

[0042] (1.2) Coupling: Dissolve the three Treponema pallidum-specific recombinant protein antigens TP15, TP17, and TP47 in 200 μL of pH=7.4 and a concentration of 0.02M Tris-HCl coupling buffer, mix well and add pretreated gold In the magnetic pa...

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Abstract

The invention belongs to the technical field of immunoassay and diagnosis and relates to a method for magnetic antibody immunoassay chemiluminescence detection of treponema pallidum antibodies. The method comprises the following steps of: using GoldMag particles as carriers, coating one or more antigens of specific recombinant proteins of treponema pallidum antibodies, TP15, TP17, TP47 and TP44.5, then adding a sample to be detected and enzyme labeled antigens, and finally adding luminescence substrates for luminescence detection. The GoldMag particle surface has a larger coupling capacity to the antigen and has characteristics of high specificity and no pollution of the enzyme linked immunoassay as well as high sensitivity of chemiluminescence, and therefore, the method has the advantages of high detection sensitivity, good specificity, wide linear range, no radioactive pollution and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis and diagnosis, and relates to a method for detecting Treponema pallidum antibodies by magnetoenzyme-immunochemiluminescence, in particular to a method for detecting Treponema pallidum antibodies using gold magnetic particles as carriers. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum (TP) infection. Treponema pallidum can invade all organs of the body, causing multiple organ lesions, dysfunction, tissue destruction and even death. Syphilis is a disease unique to humans, and patients with dominant and recessive syphilis are the source of infection. The main mode of transmission of syphilis is sexual intercourse, and very few can be infected through blood transfusion or contact with items contaminated with Treponema pallidum. Mother suffering from syphilis can be passed to the fetus. In recent years, the incidence of syphilis in my co...

Claims

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Application Information

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IPC IPC(8): G01N33/571
Inventor 崔亚丽杨璐耿婷婷陈超
Owner XIAN GOLDMAG NANOBIOTECH
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