Spittle rapid detection strip for treponema pallidum antibody

A technology for Treponema pallidum and detection test paper, which is applied in the directions of biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitability for on-site testing and high testing cost, and is suitable for on-site testing and self-testing, with high sensitivity and fast response. Effect

Inactive Publication Date: 2010-03-03
杭州艾力康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention provides a test strip for rapid detection of treponema pallidum antibody saliva, which can use saliva as a sample, and solves the problem that the existing test strip is not suitable for on-site detection and the detection cost is high

Method used

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  • Spittle rapid detection strip for treponema pallidum antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of colloidal gold pads

[0025] Prepare a colloidal gold solution with a diameter of 15nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.0, add 0.55 mg of mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to Treponemal pallidum antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin ( BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0026] Coating of nitrocellulose membrane

[0027] Dissolve the human recombinant Treponema pallidum antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can specif...

Embodiment 2

[0032] Preparation of colloidal gold pads

[0033] Prepare a colloidal gold solution with a diameter of 35nm by the chloroauric acid-trisodium citrate reduction method. After the preparation is completed, take 100ml of the colloidal gold solution in a beaker and use 0.1M K 2 CO 3 Adjust the pH to 8.2, add 1.0 mg of mouse anti-human IgG antibody (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifically bind to the Treponemal pallidum antibody, stir at room temperature for 1 hour, and add 5% bovine serum albumin ( BSA) closed for 1 hour, centrifuged at 12000rpm, 4°C for 30 minutes, discarded the supernatant, redissolved with colloidal gold solution to 100ml, spread 40cm with 1ml solution 2 Spread the solution evenly on the glass fiber membrane and dry at 37°C for 30 minutes to make a colloidal gold pad.

[0034] Coating of nitrocellulose membrane

[0035] Dissolve human recombinant Treponema pallidum antigen (Shanghai Lingchao Biotechnology Co., Ltd.) that can specifi...

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Abstract

The invention discloses a spittle rapid detection strip for a treponema pallidum antibody, comprising a baseboard, to which a sample pad, a colloidal gold pad, a nitrocellulose membrane and a sample-drawing pad are sequentially adhered. The invention is characterized in that a mouse-anti-human IgG antibody which is marked by the colloidal gold and can be integrated with the specificity of the treponema pallidum antibody is attached to the colloidal gold pad; and the nitrocellulose membrane is coated with a detection line formed by the treponema pallidum antibody and a quality control line formed by a goat-anti-mouse antiantibody which can be integrated with the specificity of the mouse-anti-human IgG antibody. The spittle rapid detection strip provided by the invention adopts the colloidalgold marking technology to detect the treponema pallidum antibody in spittle so as to determine whether the person is infected with syphilis, and has the advantages that operation is simple; reactionis quick; sensitivity is high; specificity is strong; and the spittle rapid detection strip can be applied to both on-site detection and self-detection, and is economical and practical and the like.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a test strip for rapid detection of treponema pallidum antibody saliva. Background technique [0002] Syphilis is a chronic, systemic sexually transmitted disease (VD, STD) caused by Treponema pallidum (Treponema pallidum). The vast majority are transmitted through sexual channels, clinically manifested as primary syphilis, secondary syphilis, tertiary syphilis and latent syphilis. It is a disease listed as Class B prevention and management in the "Law of the People's Republic of China on the Prevention and Control of Infectious Diseases". [0003] Treponema pallidum (Tre-ponema palidum) was discovered and reported by French scientists Schaudinn and Hoffmanu in 1905. Because it is transparent and not easy to color, it is also called pallid spirochete. Treponema pallidum is an anaerobic bacterium, which can survive and reproduce in the body for a long time. As long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N33/558G01N33/532G01N33/52
Inventor 郑隆泗李洪江
Owner 杭州艾力康医药科技有限公司
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