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Kit for detecting Treponema pallidum antibody, detection method and application thereof

A Treponema pallidum and kit technology, which is applied in biological tests, measuring devices, material testing products, etc., can solve problems such as low sensitivity and poor specificity, and achieve the effect of improving detection sensitivity

Active Publication Date: 2016-08-31
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, TP detection in conventional techniques often has the problems of low sensitivity and poor specificity

Method used

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  • Kit for detecting Treponema pallidum antibody, detection method and application thereof
  • Kit for detecting Treponema pallidum antibody, detection method and application thereof
  • Kit for detecting Treponema pallidum antibody, detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Syphilis recombinant antigen CMIA-TP-Ag8 in Example 1, source: Fapon Company.

[0062] Syphilis recombinant antigen CMIA-TP-Ag2 in Example 1, source: Fapon Company.

Embodiment 4

[0063] Syphilis recombinant antigen Tpp15 in Example 4, source: Fapon Company.

[0064] Syphilis recombinant antigen Tpp17 in Example 4, source: Fapon Company.

[0065] Syphilis recombinant antigen Tpp47 in Example 4, source: Fapon Company.

[0066] Staphylococcus aureus protein A (SPA) in Example 4, source: Yawei Company.

[0067] Goat anti-FITC polyclonal antibody, source: purchased from Beijing Baihao Biotechnology Co., Ltd.

[0068] Magnetic microspheres, source: produced by Shenzhen New Industry Biomedical Co., Ltd.

[0069] FITC: purchased from Shanghai Jining Industrial Co., Ltd.

[0070] ABEI is produced by Shenzhen New Industry Biomedical Engineering Co., Ltd.

[0071] Biotin, Streptavidin: Both were purchased from Roche.

[0072] Calibrator, source: Fapon Corporation.

[0073] Example 1

[0074] A kit for detecting Treponema pallidum antibodies, including the following components:

[0075] 1) Marker system: ABEI-labeled Treponema pallidum (Syphilis) recombina...

Embodiment 2

[0113] A test kit for detecting Treponema pallidum antibodies is basically the same as the test kit in Example 1, except that:

[0114] 4) The specific additives only contain buffer components, without binding agents and auxiliary components.

[0115] A method for detecting Treponema pallidum antibodies is basically the same as the detection method in Embodiment 1, except that the detection kit of this embodiment is used for detection.

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Abstract

The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis and detection, in particular to a kit for detecting Treponema pallidum antibodies, a detection method and application thereof. Background technique [0002] Syphilis is a chronic, systemic sexually transmitted disease caused by Treponema pallidum. Also known as Treponemia pallidum (TP), it was discovered and reported by French scientists Schaudinn and Hoffmanu in 1905. In the taxonomy, it belongs to the order Spirochaetales, Treponemataceae, Genus Treponema. The cells are slender, with 6 to 12 evenly arranged spirals, 5 to 20 μm in length, 6 to 10 μm in average length, and 0.15 μm in transverse diameter. The movement is slow and regular, and the dyes commonly used in the laboratory are not easy to be colored. Dark field microscopes can be used. Or phase contrast microscope to observe the bacteria. Mainly through sexual transmission, clinically can be manifested as primary syphilis, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/571G01N33/543
CPCG01N33/54393G01N33/571
Inventor 饶微袁锦云刘晓森方中刚罗凯李婷华
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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