Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting treponema pallidum antibody as well as detection method and application thereof

A Treponema pallidum and kit technology, which is applied in biological tests, measuring devices, material testing products, etc., can solve problems such as low sensitivity and poor specificity, and achieve the effect of improving detection sensitivity

Active Publication Date: 2015-06-10
SHENZHEN NEW INDS BIOMEDICAL ENG
View PDF12 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, TP detection in conventional techniques often has the problems of low sensitivity and poor specificity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting treponema pallidum antibody as well as detection method and application thereof
  • Kit for detecting treponema pallidum antibody as well as detection method and application thereof
  • Kit for detecting treponema pallidum antibody as well as detection method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The source of the Syphilis recombinant antigen CMIA-TP-Ag8 in Example 1: Fapon Company.

[0062] The source of the Syphilis recombinant antigen CMIA-TP-Ag2 in Example 1: Fapon Company.

Embodiment 4

[0063] The source of the Syphilis recombinant antigen Tpp15 in Example 4: Fapon Company.

[0064] The source of the Syphilis recombinant antigen Tpp17 in Example 4: Fapon Company.

[0065] The source of the Syphilis recombinant antigen Tpp47 in Example 4: Fapon Company.

[0066] Staphylococcus aureus protein A (SPA) in Example 4, source: Yawei Company.

[0067] Goat anti-FITC polyclonal antibody, source: purchased from Beijing Baihao Biotechnology Co., Ltd.

[0068] Magnetic microspheres, source: produced by Shenzhen New Industry Biomedical Co., Ltd.

[0069] FITC: purchased from Shanghai Ji Ning Industrial Co., Ltd.

[0070] ABEI is produced by Shenzhen New Industry Biomedical Engineering Co., Ltd.

[0071] Biotin, streptavidin: all purchased from Roche.

[0072] Calibrator, source: Fapon Company.

[0073] Example 1

[0074] A test kit for detecting Treponema pallidum antibodies, comprising the following components:

[0075] 1) Marker system: ABEI-labeled Treponema pa...

Embodiment 2

[0113] A test kit for detecting Treponema pallidum antibody, which is basically the same as the test kit in Example 1, except that:

[0114] 4) The specific additive only contains buffer components, without binding agents and auxiliary components.

[0115] A method for detecting Treponema pallidum antibody is basically the same as the detection method in Example 1, except that the detection kit of this embodiment is used for detection.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a kit for detecting a treponema pallidum antibody as well as a detection method and an application thereof, and belongs to the technical field of in vitro diagnosis and detection. The kit consists of the following components: (1) a marker system: including a treponema pallidum recombinant antigen 1 in direct or indirect connection to a marking tracer, or staphylococcus aureus A protein in direct or indirect connection to the marking tracker; (2) a bridged compound system: including a treponema pallidum recombinant antigen 2 in direct or indirect connection to a first bridged compound; and (3) a magnetic microsphere system: comprising a magnetic microsphere in direct or indirect connection to a second bridged compound, wherein the first bridged compound is combined with the second bridged compound. The kit and the detection method disclosed by the invention can promote the full implementation of conjugation reaction between antigen and antibody; and detection sensitivity can be improved.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis and detection, in particular to a kit for detecting Treponema pallidum antibody, a detection method and application thereof. Background technique [0002] Syphilis is a chronic, systemic sexually transmitted disease caused by Treponema pallidum. Also known as Treponemia pallidum (TP), it was discovered and reported by French scientists Schaudinn and Hoffmanu in 1905. In taxonomy, it belongs to Spirochaetales, Treponemataceae, and Genus Treponema. The bacteria are slender, with 6 to 12 helices evenly arranged, 5 to 20 μm long, with an average length of 6 to 10 μm, and a transverse diameter of 0.15 μm or so. The movement is slow and regular. The dyes commonly used in the laboratory are not easy to color, and dark field microscopy can be used Or observe the bacteria with a phase contrast microscope. It is mainly transmitted through sexual channels, and clinically it can be manifested as...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/571G01N33/543
CPCG01N33/54393G01N33/571
Inventor 饶微袁锦云刘晓森方中刚罗凯李婷华
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com