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Four-in-one combined detection test paper box and preparation process

A combined detection and preparation technology, applied in the field of medical testing, can solve the problems of high price, cumbersome operation, long reaction time in the measurement process, etc., and achieve the effect of less sample demand.

Inactive Publication Date: 2017-01-04
HANGZHOU BIOTEST BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above-mentioned determination process takes a long time to react, the reaction conditions are relatively limited, the operation is cumbersome, and instrument-assisted measurement is required, the price is expensive, and one test can only complete the detection of one antigen or antibody

Method used

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  • Four-in-one combined detection test paper box and preparation process
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  • Four-in-one combined detection test paper box and preparation process

Examples

Experimental program
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Effect test

preparation example Construction

[0049] (2) The preparation process of the four-in-one joint detection test kit

[0050] The preparation process of the four-in-one combined detection test kit includes the following steps:

[0051] (1) Stick the nitrocellulose membrane on the plastic card, then use the protein stabilizer to mark the antibody / antigen combination for detection, prepare a solution, and spray the solution evenly on the glued nitrocellulose membrane through the machine After drying, the processed sheet containing the quality control area and the detection area is obtained for use;

[0052] (2) Link the antibody / antigen and colloidal gold / latex particles together, and prepare a solution, process the solution onto the carrier by a machine, and finally dry it to obtain the processed marking pad for use;

[0053] (3) process the glass fiber with the sample pad treatment solution, after drying, obtain the processed sample pad for subsequent use;

[0054] (4) Configure sample diluent;

[0055] (5) Ass...

specific Embodiment 1

[0113] 1. Preparation process: Prepare the four-in-one joint detection test paper box according to the above preparation process. The length of the test paper strip is 60mm, the width is 3mm, and the distance between the quality control line and the detection line and the bottom edge is C / T1 / T2 / T3 / T4: 38.5mm / 35.5mm / 32.5mm / 29.5mm / 26.5mm, the components are as follows:

[0114] (1) The main raw materials of each component of the marking pad are as follows:

[0115]

[0116] (2) The main raw materials of each component of the detection line are as follows:

[0117] 1) HIV antibody detection line component content:

[0118]

[0119] 2) Content of the components of the hepatitis B surface antigen detection line

[0120]

[0121]

[0122] 3) HCV antibody detection line component content

[0123]

[0124] 4) Component content of Treponema pallidum antibody detection line

[0125]

[0126] (3) The main raw materials of each component of the quality control line ...

specific Embodiment 2

[0162] 1. Preparation process: Prepare the four-in-one combined detection test kit according to the above preparation process, and the detection line combination is T1: HIV 1 / 2 antibody detection line (HIV), T2 hepatitis B surface antigen detection line (HBsAg) , T3 hepatitis C antibody detection line (HCV), T4 Treponema pallidum antibody detection line (SYP), the length of the test strip is 65mm, the width is 5mm, and the distance between the quality control line and the detection line and the bottom edge is C / T1 / T2 / T3 / T4: ​​38.5mm / 35.5mm / 32.5mm / 29.5mm / 26.5mm, the components are as follows:

[0163] (1) The main raw materials of each component of the marking pad are as follows:

[0164]

[0165]

[0166] (2) The main raw materials of each component of the detection line are as follows:

[0167] 1) HIV antibody detection line component content:

[0168]

[0169] 2) Content of the components of the hepatitis B surface antigen detection line

[0170]

[0171] 3) ...

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Abstract

The invention relates to a four-in-one combined detection test paper box. A coating film of a test paper strip is provided with a quality control region and a detection region in sequence from top to bottom; the quality control region and the detection region are provided with a quality control line and a detection line respectively; and the detection line is composed of an AIDS (Acquired Immune Deficiency Syndrome) virus 1 / 2 type antibody detection line, a hepatitis B surface antigen detection line, a hepatitis C antibody detection line and a treponema pallidum antibody detection line, which are arrayed in sequence from top to bottom. The invention further relates to a preparation process of the test paper box. The preparation process comprises the steps of preparing a label pad, preparing the detection line, preparing the quality control line, preparing a sample pad and preparing a sample diluted solution. According to the product provided by the invention, four infectious diseases can be simultaneously detected on one test paper strip in one step; and the required amount on samples is less and the detection is rapid and accurate.

Description

technical field [0001] The invention belongs to the field of medical testing, and relates to a four-in-one combined detection test paper box for HIV 1 / 2 (HIV1 / 2) antibody, Treponema pallidum antibody, hepatitis C antibody and hepatitis B surface antigen and a preparation process. Background technique [0002] In 1971, Engvall and Perlmann published an article on the use of ELISA (enzyme linked immunosorbent assay, enzyme-linked immunosorbent assay) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology for antigen localization developed in 1966 into the detection of trace substances in liquid specimens. The basic principle of the determination method is: 1. Make the antigen or antibody bind to the surface of a certain solid phase carrier and maintain its immunological activity; 2. Make the antigen or antibody link with a certain enzyme to form an enzyme-labeled antigen or antibody. The labeled antigen or antibody not only retains its imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/576G01N33/571G01N33/569
CPCG01N33/558G01N33/531G01N33/56988G01N33/571G01N33/5767
Inventor 叶春生张振兴侯鲁娜王桢煜郑志明周英建戴铖
Owner HANGZHOU BIOTEST BIOTECH CO LTD
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