Treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and preparation method thereof

Abstract

The invention relates to a treponema pallidum IgG antibody biotin avidin enzyme-linked immune detection kit and a preparation method thereof, which relates to the syphilis detection. The kit comprises a concentrated washing liquid bottle, a negative and positive control bottle, a biotin mark antihuman IgG specific fragment gamma chain monoclonal antibody bottle, a horseradish peroxidase mark avidin bottle, a TMB coloured solution bottle A, a coloured solution bottle B, a reaction stopping solution bottle, a micropore plate coated with recombinant antigen. The treponema pallidum has infectious standard strain Nichol strain and treponema pallidum non-infectious standard strain Reiter strain, by using a proteomics method, the treponema pallidum proteomics can be separated through dimensional electrophoresis, protein with strong immunogenicity can be identified by different patient or infectious animal serum, and then an antigen marker can be searched. The antigen marker is used for syphilis confirmation test, so that diagnosis sensitivity and specificity can be increased, a window stage is shortened. The treponema pallidum antibody can be sensitively detected and enables quantitative measurement, and has the advantages of cheap price, simple operation and accurate result.

Description

technical field [0001] The invention relates to the detection of syphilis, in particular to a Treponema pallidum IgG antibody biotin avidin ELISA kit and a preparation method thereof. Background technique [0002] Syphilis is a sexually transmitted disease caused by Treponema pallidum. In recent years, the incidence rate in China has remained high. The prevention and control of syphilis has become one of the main tasks of public health services in my country. [0003] The laboratory diagnosis methods of syphilis are mainly as follows ([1] Lin Lirong, Yang Bo, Pan Xitao, etc. Selection of serological detection methods for syphilis in patients with potential blood-borne transmission. Chinese Journal of Hospital Infection. 2010, 20(10): 1491-1494): [0004] (1) Etiological detection: In the early stage of Treponema pallidum infection, when the syphilis antibody has not yet been produced or the content is low, dark field microscopy to search for Treponema pallidum has become th...

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Problems solved by technology

[0004] (1) Etiological detection: In the early stage of Treponema pallidum infection, when the syphilis antibody has not yet been produced or the content is low, dark field microscopy to search for Treponema pallidum has become the earliest laboratory diagnostic method, but it is easily affected by the sampling technique, sampling site, and Treponema pallidum in the sample. Affected by many factors such as content, topical medication and delivery time, the sensitivity is low

[0005] (2) Antibody detection test: Treponema pallidum cannot be cultured in vitro, and the diagnosis mainly depends on laboratory examination. The sensitivity and specificity of the existing serological detection are not high, and any detection method has defects. high

[0006] The antigens used for syphilis serological testing are mainly recombinant antigens TPN15, TPN17, TPN37, TPN44.5, and TPN47 ([2] Lin, L.R., Z.G.Fu, et al. "Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TPN17and TPN47."Diagnostic microbiology and infectious disease.2010.68(3):193-200.), most of these antigens are derived from the outer membrane of Treponema pallidum, but it cannot be distinguished whether it is caused by pathogenic Treponema

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