Treponema pallidum antibody detection card, kit and detection method
A technology for the detection of Treponema pallidum and antibodies, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and difficult clinical acceptance, and achieve accurate results, reduce heterophilic antibody interference, and good specificity
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Embodiment 1
[0031] A Treponema pallidum antibody detection card, comprising a detection test paper and a plastic shell 1 tightly wrapped around the outer periphery of the test paper, the detection test paper includes a bottom plate 2, on which a sample pad 3, a binding pad 4, and nitrocellulose are sequentially laid from left to right Membrane 5 and absorbent paper 6, the end of sample pad 3 close to binding pad 4, the two ends of binding pad 4, the two ends of nitrocellulose membrane 5, and the end of absorbent paper 6 close to nitrocellulose membrane 5 are all rectangular. The right-angled ends of the pad 3, the bonding pad 4, the nitrocellulose membrane 5 and the absorbent paper 6 are sequentially matched and fixedly connected, and the length of the right-angled ends is 1-2mm, and the detection belt 7 and the For the control belt 8, the plastic shell 1 is provided with a sample inlet 11 and an observation window 12, the sample inlet 11 corresponds to the position of the sample pad 3, th...
Embodiment 2
[0046] A test kit comprising the Treponema pallidum antibody detection card in Example 1, the test kit includes a detection card, an aluminum foil bag, a straw, a drop bottle filled with buffer solution PBS, and an instruction manual.
[0047] A detection method utilizing the Treponema pallidum antibody detection card of embodiment 1, comprising the steps of:
[0048] C1, prepare the test sample, the test sample is one of serum and plasma;
[0049] C2, drop 20 μL of the test sample in C1 on the sample pad 3, then add dropwise 60-70 μL of PBS buffer solution (0.01mol / L, pH7.4), let it stand for 15 minutes, and observe the detection zone 7 and the control zone 8. Color situation;
[0050] C3, if both the detection zone 7 and the control zone 8 are colored, the test sample contains Treponema pallidum antibodies; if the test zone 7 does not develop color and the control zone 8 develops color, then the test sample does not contain Treponema pallidum antibodies; if the The control...
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