Treponema pallidum antibody detection card, kit and detection method
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A technology for the detection of Treponema pallidum and antibodies, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and difficult clinical acceptance, and achieve accurate results, reduce heterophilic antibody interference, and good specificity
Inactive Publication Date: 2019-06-25
PEOPLES HOSPITAL OF HENAN PROV
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Treponema pallidum examination is suitable for patients with primary and secondary syphilis lesions. Due to the limitation of materials and special microscopes, it takes a long time and is difficult to be accepted clinically.
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Embodiment 1
[0031] A Treponema pallidum antibody detection card, comprising a detection test paper and a plastic shell 1 tightly wrapped around the outer periphery of the test paper, the detection test paper includes a bottom plate 2, on which a sample pad 3, a binding pad 4, and nitrocellulose are sequentially laid from left to right Membrane 5 and absorbent paper 6, the end of sample pad 3 close to binding pad 4, the two ends of binding pad 4, the two ends of nitrocellulose membrane 5, and the end of absorbent paper 6 close to nitrocellulose membrane 5 are all rectangular. The right-angled ends of the pad 3, the bonding pad 4, the nitrocellulose membrane 5 and the absorbent paper 6 are sequentially matched and fixedly connected, and the length of the right-angled ends is 1-2mm, and the detection belt 7 and the For the control belt 8, the plastic shell 1 is provided with a sample inlet 11 and an observation window 12, the sample inlet 11 corresponds to the position of the sample pad 3, th...
Embodiment 2
[0046] A test kit comprising the Treponema pallidum antibody detection card in Example 1, the test kit includes a detection card, an aluminum foil bag, a straw, a drop bottle filled with buffer solution PBS, and an instruction manual.
[0047] A detection method utilizing the Treponema pallidum antibody detection card of embodiment 1, comprising the steps of:
[0048] C1, prepare the test sample, the test sample is one of serum and plasma;
[0049] C2, drop 20 μL of the test sample in C1 on the sample pad 3, then add dropwise 60-70 μL of PBS buffer solution (0.01mol / L, pH7.4), let it stand for 15 minutes, and observe the detection zone 7 and the control zone 8. Color situation;
[0050] C3, if both the detection zone 7 and the control zone 8 are colored, the test sample contains Treponema pallidum antibodies; if the test zone 7 does not develop color and the control zone 8 develops color, then the test sample does not contain Treponema pallidum antibodies; if the The control...
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Abstract
The invention belongs to the field of biological detection technologies and relates to a treponema pallidum antibody detection card, a kit and a detection method. The treponema pallidum antibody detection card comprises test paper and a plastic shell; the test paper comprises a bottom plate; a sample pad, a bonding pad, nitrocellulose membrane and absorbent paper are sequentially arranged on the bottom plate from the left to right of the bottom plate; the nitrocellulose membrane is provided with a detection zone and a control zone from the left to right of the nitrocellulose membrane; a glassfibre membrane is soaked by a sample pad treatment liquid and dried, so that the sample pad can be obtained; a mixed immunogold solution is sprayed onto the glass fibre membrane, so that the bonding pad can be obtained; a treponema pallidum recombinant antigen diluted by a coating solution is sprayed onto the nitrocellulose membrane, so that the detection zone can be formed; and an anti-chicken IgY antibody diluted by the coating solution is sprayed onto the nitrocellulose membrane, so that the control zone can be formed. The detection card of the invention has the advantages of convenience inapplication and accurate results, and provides a basis for the accurate treatment of syphilis in the next step, and is suitable for being popularized and applied on a large scale.
Description
technical field [0001] The invention belongs to the technical field of biological detection, in particular to a treponema pallidum antibody detection card, a kit and a detection method. Background technique [0002] Treponema pallidum (TP) is the main pathogen that causes the sexually transmitted disease syphilis. It was first discovered by Schandim et al. in 1905. It spreads through direct contact of human mucous membranes or damaged skin with infectious lesions of patients, and most of the invasion sites are the genitals. The clinical manifestations of this pathogen are extremely complex, almost invading every organ of the body, causing multiple organ damage. In the early stage, it mainly invades the skin and mucous membranes, and in the late stage, it can invade blood vessels, central nervous system and various organs of the whole body, and can be transmitted to the fetus through the placenta, which is extremely harmful. [0003] Syphilis is prevalent all over the world....
Claims
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