Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin

A technology of mycotoxins and Enterococcus faecalis, applied in the direction of application, bacteria, and microbial-based methods, can solve the problem of high addition rate and achieve the effect of strong adsorption capacity and rapid adsorption

Active Publication Date: 2010-08-04
山东宝来利来生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic adsorbent hydrated sodium calcium aluminosilicate (HSCAS) can adsorb aflatoxin, and has a high affinity and adsorption capacity with aflatoxin in aqueous solution. The advantage of clay-type adsorbents (such as bentonite, zeolite, HSCAS) is the cost Inexpensive, unfortunately these products must be included at higher rates in the diet to be effective

Method used

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  • Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin
  • Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin
  • Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening, cultivation and detection of mycotoxin adsorption of Enterococcus faecalis SRG CCTCC M 2010046 capable of adsorbing mycotoxins:

[0029] 1. Main instrument reagents

[0030] Reagents: MRS medium, benzene, hexonitrile, sodium hypochlorite solution, AFB1 detection kit, hydrated aluminosilicate;

[0031] Instruments and main consumables: 10mL centrifuge tube, large, medium and small tips, microplate reader (Thermo).

[0032] 2. Test strains

[0033] ①Original strains in the laboratory: LP, 7340, RDH, FLQ, ZYRLQ, SRG, RS1XL.

[0034] ② Strains isolated from chicken intestines and cow manure: Aseptically take 5g of chicken intestine contents / fresh cow manure, add it to a conical flask with glass beads filled with 50mL sterilized normal saline, mix well and then add 2% CaCO 3 The MRS medium plate was streaked and separated, cultured at 37°C for 24 hours, and the Ca 2+ Continue to streak and purify the colonies in the plate, then observe the morphology...

Embodiment 2

[0077] The research of embodiment 2 Enterococcus faecalis SRG characteristics

[0078] 1 Materials and methods

[0079] 1.1 Test material

[0080] Enterococcus faecalis: preserved in the China Center for Type Culture Collection, the preservation number is CCTCC M 2010046; Escherichia coli O 1 , Chicken Staphylococcus aureus-C56011: all purchased from the Central Prison Institute;

[0081] Test equipment: electronic balance, water bath, microwave oven, binocular microscope, dryer, high-speed refrigerated centrifuge, horizontal centrifuge, vertical purification bench, etc.

[0082] 1.2 Test method

[0083] 1.2.1 Gram staining method

[0084] Dip the diluted solution on the MRS agar plate to streak and inoculate, adopt the aerobic method, pyrogallic acid method and candle jar method respectively, and incubate at a constant temperature in a 37°C incubator for 24 hours. Pick 5 typical colonies for each method to make Gram Staining, microscopic examination, and pure culture.

...

Embodiment 3

[0125] Embodiment 3 prepares biological mildew remover

[0126] Proceed as follows:

[0127] (1) Fermentation:

[0128] (i) Strain: Enterococcus faecalis SRG CCTCC M 2010046 was selected;

[0129] (ii) Slant culture: inoculate the freeze-dried powder strains on a solid slant medium, and culture at 37°C for 24 hours;

[0130] (iii) Primary seed culture: Put the cultured slant into 80mL seed liquid culture medium with an inoculation loop under sterile conditions, and culture it statically for 12 hours at 37°C to obtain the primary seed liquid ;

[0131] (iv) Expanded cultivation: with an inoculum amount of 5% (v / v), the primary seed liquid was placed in 800 mL of seed liquid medium, and cultured at 37° C. for 8 hours to obtain a secondary seed liquid;

[0132] (v) Fermentation tank culture: with the inoculum amount of 5% (v / v), the secondary seed solution was placed in the liquid fermentation medium, and cultured statically for 16 hours at 37°C;

[0133] (vi) Collect fermen...

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Abstract

The invention discloses enterococcus faecalis capable of absorbing mycotoxin. The strain is named enterococcus faecalis SRG and is collected in China Center for Type Culture Collection on 2nd, March, 2010, and the collection number of the strain is CCTCC M 2010046. The enterococcus faecalis can absorb mycotoxin, has stronger adsorption capability and can be independently used as the mould-removing agent of a feed or can be compounded with hydrated aluminosilicate to be used as the mould-removing agent of the feed. The enterococcus faecalis has the basic biological health-care function while removing moulds. The enterococcus faecalis is compounded with the hydrated aluminosilicate with stronger mold adsorption capacity, and thereby, the mould-removing effect is achieved by physical adsorption, and the effects of mould proofing, mould removing and health care can be achieved by the bioactivity of the enterococcus faecalis.

Description

technical field [0001] The invention relates to a strain of enterococcus faecalis capable of adsorbing mycotoxins and its application in adsorbing mycotoxins. Background technique [0002] Feed raw materials or compound feeds are easily contaminated with various molds during production, processing, storage, and transportation. Under suitable conditions, molds multiply and cause mildew. At present, the proportion of mycotoxins in the world's feed grains is as high as 25%. In addition to causing significant economic losses to the animal husbandry industry, some mycotoxins are also carcinogenic or teratogenic, and can be transmitted to humans through the milk of edible meat. In China, the feed and feed raw materials were sampled to investigate the contamination of mycotoxins, and it was found that six kinds of mycotoxins (aflatoxin, T-2 toxin, vomitoxin, zearalenone, ochratoxin, smoke Aspergillus toxin) is ubiquitous in the inspected feed and feed ingredients. The detection r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/00C12R1/01
Inventor 单宝龙谷巍张建梅
Owner 山东宝来利来生物工程股份有限公司
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